Van Huy Tran1, Thi Minh Thi Ha2, Phan Tuong Quynh Le3, Trung Nam Phan4, Thi Nhu Hoa Tran5. 1. Center of Gastrointestinal Endoscopy, Hue University Hospital, Hue, Vietnam; Department of Internal Medicine, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam. 2. Department of Medical Genetics, Hue University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen Street, Hue, Vietnam. Electronic address: haminhthi@huemed-univ.edu.vn. 3. Department of Medical Genetics, Hue University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen Street, Hue, Vietnam. 4. Center of Gastrointestinal Endoscopy, Hue University Hospital, Hue, Vietnam. 5. Department of Microbiology, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam.
Abstract
OBJECTIVES: The prevalence of clarithromycin (CLR)-resistant Helicobacter pylori is increasing worldwide, including in Vietnam. The aims of this study were to determine point mutations in the 23S rRNA domain V of clinical H. pylori strains in central Vietnam, to estimate the prevalence of phenotypic CLR resistance and to assess the association between 23S rRNA domain V genotype and CLR-resistant phenotype. METHODS: Sequencing of the 23S rRNA domain V of H. pylori strains from gastric biopsy specimens was performed for 185 patients with H. pylori-positive chronic gastritis, of which 104 samples were subjected to susceptibility testing to determine CLR resistance. RESULTS: A total of 24 types of point mutation were detected. A2143G and A2142G mutations were observed in 40.5% and 4.3%, respectively. New point mutations were detected (C2041T, C2083T, C2191T, G2220A, G2225A, G2240A, C2273T, T2276C, G2287A, C2399T, A2445G and C2622T). 23S rRNA domain V genotypes were diversified, with combinations of two or more point mutations as well as single point mutations. The rate of phenotypic CLR resistance was 53.8%, increasing from 40.4% in 2012-2014 to 70.2% in 2015-2017 (P=0.0045). A2143G and A2142G accounted for 89.3% of phenotypically CLR-resistant H. pylori isolates. CONCLUSIONS: A diversity of point mutations in the 23S rRNA domain V was observed in clinical H. pylori isolates. The rate of phenotypically CLR-resistant H. pylori is significantly increasing in central Vietnam. Further research is necessary to clarify the role of the combination of 23S rRNA domain V mutations in the molecular mechanism of CLR resistance.
OBJECTIVES: The prevalence of clarithromycin (CLR)-resistant Helicobacter pylori is increasing worldwide, including in Vietnam. The aims of this study were to determine point mutations in the 23S rRNA domain V of clinical H. pylori strains in central Vietnam, to estimate the prevalence of phenotypic CLR resistance and to assess the association between 23S rRNA domain V genotype and CLR-resistant phenotype. METHODS: Sequencing of the 23S rRNA domain V of H. pylori strains from gastric biopsy specimens was performed for 185 patients with H. pylori-positive chronic gastritis, of which 104 samples were subjected to susceptibility testing to determine CLR resistance. RESULTS: A total of 24 types of point mutation were detected. A2143G and A2142G mutations were observed in 40.5% and 4.3%, respectively. New point mutations were detected (C2041T, C2083T, C2191T, G2220A, G2225A, G2240A, C2273T, T2276C, G2287A, C2399T, A2445G and C2622T). 23S rRNA domain V genotypes were diversified, with combinations of two or more point mutations as well as single point mutations. The rate of phenotypic CLR resistance was 53.8%, increasing from 40.4% in 2012-2014 to 70.2% in 2015-2017 (P=0.0045). A2143G and A2142G accounted for 89.3% of phenotypically CLR-resistant H. pylori isolates. CONCLUSIONS: A diversity of point mutations in the 23S rRNA domain V was observed in clinical H. pylori isolates. The rate of phenotypically CLR-resistant H. pylori is significantly increasing in central Vietnam. Further research is necessary to clarify the role of the combination of 23S rRNA domain V mutations in the molecular mechanism of CLR resistance.
Authors: Trung Thien Tran; Anh Tuan Nguyen; Duc Trong Quach; Dao Thi-Hong Pham; Nga Minh Cao; Uyen Thi-Hong Nguyen; An Nguyen-Thanh Dang; Minh Anh Tran; Loc Huu Quach; Khiem Thien Tran; Nhan Quang Le; Viet Van Ung; Minh Ngoc-Quoc Vo; Danh Thanh Nguyen; Kha Dong Ngo; Trung Le Tran; Vy Thuy Nguyen Journal: BMC Microbiol Date: 2022-02-03 Impact factor: 3.605