Nadeem Anjum1, Arbab Jehangir1, Yu Liu1. 1. Department of Biochemistry, International School, Clinical Laboratory of Over-sea Chinese Hospital, Medical School, Jinan University, 510632, China.
Abstract
OBJECTIVE: To determine the risk of type II diabetes (T2D) with two transcription factor 7-like 2 gene (TCF7L2) variants in the Han nationality population of Guangdong, China. STUDY DESIGN: Case control study. PLACE AND DURATION OF STUDY: International School, Clinical Laboratory of Over-sea Chinese Hospital, Department of Biochemistry, Medical School, Jinan University, China, from July 2014 to June 2016. METHODOLOGY: Two single nucleotide polymorphisms (SNPs) of gene TCF7L2 were genotyped by PCR amplification and sequencing of the specific DNA fragments, rs7903146 and rs12255372, by PCR amplification and sequencing of the specific DNA fragments in 339 T2D patients and 191 control subjects. Odds ratio was determined. RESULTS: The minor allele of the two variants was significantly associated with T2D; the risk to develop T2D for rs7903146 (IVS3C >T) was found to be greater than that for rs12255372 (IVS4G >T), with allelic odds ratio (OR) of 1.39 and 1.27 respectively. Furthermore, T2D risk associated with homozygosity of the minor alleles was found to be greater than that for heterozygosity. CONCLUSION: The two varients of the gene TCF7L2 are important genetic risk factors for the T2D development in the Han ethnic group in China.
OBJECTIVE: To determine the risk of type II diabetes (T2D) with two transcription factor 7-like 2 gene (TCF7L2) variants in the Han nationality population of Guangdong, China. STUDY DESIGN: Case control study. PLACE AND DURATION OF STUDY: International School, Clinical Laboratory of Over-sea Chinese Hospital, Department of Biochemistry, Medical School, Jinan University, China, from July 2014 to June 2016. METHODOLOGY: Two single nucleotide polymorphisms (SNPs) of gene TCF7L2 were genotyped by PCR amplification and sequencing of the specific DNA fragments, rs7903146 and rs12255372, by PCR amplification and sequencing of the specific DNA fragments in 339 T2D patients and 191 control subjects. Odds ratio was determined. RESULTS: The minor allele of the two variants was significantly associated with T2D; the risk to develop T2D for rs7903146 (IVS3C >T) was found to be greater than that for rs12255372 (IVS4G >T), with allelic odds ratio (OR) of 1.39 and 1.27 respectively. Furthermore, T2D risk associated with homozygosity of the minor alleles was found to be greater than that for heterozygosity. CONCLUSION: The two varients of the gene TCF7L2 are important genetic risk factors for the T2D development in the Han ethnic group in China.