Gabriel Mulinari-Santos1,2,3,4, Fábio Roberto de Souza Batista1, Franziska Kirchweger2,3, Stefan Tangl2,3, Reinhard Gruber3,4,5, Roberta Okamoto6. 1. Department of Oral Surgery and Integrated Clinic, Araçatuba Dental School, Universidade Estadual Paulista "Júlio de Mesquita Filho", Araçatuba, Brazil. 2. Karl Donath Laboratory for Hard Tissue and Biomaterial Research, Department of Oral Surgery, School of Dentistry, Medical University of Vienna, Vienna, Austria. 3. Austrian Cluster for Tissue Regeneration, Vienna, Austria. 4. Department of Oral Biology, Dental School, Medical University of Vienna, Vienna, Austria. 5. Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland. 6. Department of Basic Science, Araçatuba Dental School, Universidade Estadual Paulista "Júlio de Mesquita Filho", Araçatuba, Brazil.
Abstract
OBJECTIVE: Hypertension is not only associated with cardiovascular diseases but also with alterations in bone quality. Hypertension therefore might be a risk factor for osseointegration. Preclinical studies suggest that losartan, an angiotensin II receptor blocker widely used to treat hypertension, has a beneficial effect in graft consolidation. Here, we determine the effect of hypertension and losartan on osseointegration. METHODS: We used spontaneously hypertensive rats (SHR) and normotensive Wistar albinus rats receiving losartan (30 mg/kg, p.o.) or left untreated. After 1 week, titanium miniscrews were inserted into the tibia. Sixty days after implantation, implant stability was evaluated by removal torque measurement considered the primary endpoint. Microcomputed tomography and histomorphometric analysis were secondary endpoints. RESULTS: Losartan increased the removal torque in the hypertensive SHR group to levels of the Wistar controls. While the cortical parameters of osseointegration remained unchanged, losartan increased medullary bone formation. Microcomputed tomography revealed a higher bone volume per tissue volume and trabecular thickness in the SHR rats treated with losartan. Histomorphometric analysis further showed that losartan significantly increased the thickness of newly formed bone in medullary area in hypertensive SHR rats. Losartan did not significantly alter the parameters of osseointegration in normotensive rats. CONCLUSIONS: The data presented suggest that the angiotensin II receptor antagonist losartan increases the medullary parameters of osseointegration in a tibia model of spontaneously hypertensive rats. Considering the study limitations, understanding the impact of hypertension and the respective drugs on osseointegration requires further research.
OBJECTIVE:Hypertension is not only associated with cardiovascular diseases but also with alterations in bone quality. Hypertension therefore might be a risk factor for osseointegration. Preclinical studies suggest that losartan, an angiotensin II receptor blocker widely used to treat hypertension, has a beneficial effect in graft consolidation. Here, we determine the effect of hypertension and losartan on osseointegration. METHODS: We used spontaneously hypertensiverats (SHR) and normotensive Wistar albinus rats receiving losartan (30 mg/kg, p.o.) or left untreated. After 1 week, titanium miniscrews were inserted into the tibia. Sixty days after implantation, implant stability was evaluated by removal torque measurement considered the primary endpoint. Microcomputed tomography and histomorphometric analysis were secondary endpoints. RESULTS:Losartan increased the removal torque in the hypertensive SHR group to levels of the Wistar controls. While the cortical parameters of osseointegration remained unchanged, losartan increased medullary bone formation. Microcomputed tomography revealed a higher bone volume per tissue volume and trabecular thickness in the SHR rats treated with losartan. Histomorphometric analysis further showed that losartan significantly increased the thickness of newly formed bone in medullary area in hypertensive SHR rats. Losartan did not significantly alter the parameters of osseointegration in normotensive rats. CONCLUSIONS: The data presented suggest that the angiotensin II receptor antagonist losartan increases the medullary parameters of osseointegration in a tibia model of spontaneously hypertensiverats. Considering the study limitations, understanding the impact of hypertension and the respective drugs on osseointegration requires further research.
Authors: Isadora Prado Cano; Thiago José Dionisio; Tânia Mary Cestari; Adriana Maria Calvo; Bella Luna Colombini-Ishikiriama; Flávio Augusto Cardoso Faria; Walter Luiz Siqueira; Carlos Ferreira Santos Journal: PLoS One Date: 2019-05-22 Impact factor: 3.240