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Literature DB >> 3026483

The cleavage of single-stranded DNA by the isoschizomeric restriction endonuclease HhaI and CfoI.

Abstract

The cleavage of single-stranded (ss) M13mp8(+) DNA by the isoschizomeric restriction endonucleases HhaI and CfoI has been investigated. The two enzymes differ considerably in their ability to cleave ssDNA. HhaI cleaves ssDNA about two orders of magnitude faster than does CfoI, although both enzymes show the same activity when assayed on double-stranded DNA. From the cleavage of oligonucleotides and of M13mp8(+) DNA fragments it is concluded that cleavage of ssDNA occurs via transiently formed double-stranded hairpin structures. A rough correlation exists between the stability of the secondary structures and the cleavage efficiency.

Entities: Chemical

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Year: 1987 PMID： 3026483 DOI： 10.1016/0167-4781(87)90025-x

Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002

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Cited

4 in total

4. Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α₁β₂γ_2L GABA_A receptor and spontaneous neuronal network function in vitro.

Authors: Anke M Tukker; Lianne M S Bouwman; Regina G D M van Kleef; Hester S Hendriks; Juliette Legler; Remco H S Westerink
Journal: Sci Rep Date: 2020-03-24 Impact factor: 4.379

4 in total

The cleavage of single-stranded DNA by the isoschizomeric restriction endonuclease HhaI and CfoI.

1. Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120.

2. Targeted DNA methylation of neurodegenerative disease genes via homology directed repair.

3. Binary control of enzymatic cleavage of DNA origami by structural antideterminants.

4. Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α₁β₂γ_2L GABA_A receptor and spontaneous neuronal network function in vitro.

The cleavage of single-stranded DNA by the isoschizomeric restriction endonuclease HhaI and CfoI.

1. Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120.

2. Targeted DNA methylation of neurodegenerative disease genes via homology directed repair.

3. Binary control of enzymatic cleavage of DNA origami by structural antideterminants.

4. Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro.

4. Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α₁β₂γ_2L GABA_A receptor and spontaneous neuronal network function in vitro.