Decline pattern and risk assessment of cyenopyrafen in different varieties of Asian pear using liquid chromatography and tandem mass spectrometry.

The dissipation pattern of a commercial cyenopyrafen formulation sprayed at the recommended dose on Asian pears (two different species) grown at two different sites was investigated using liquid chromatography-ultraviolet detection. Samples collected randomly over 14 days were extracted using acetone, partitioned using n-hexane/dichloromethane (8/2, v/v), and purified using a Florisil solidphase extraction cartridge. The residues in field-incurred samples were confirmed via liquid chromatography-tandem mass spectrometry. The method was validated in terms of excellent linearity in the solvent (R 2=1); moreover, satisfactory recoveries (89.0-107.3%) were obtained at three fortification levels with a relative standard deviation (RSD)≤5.0% and the limits of detection and quantification of 0.0033 and 0.01 mg/kg, respectively. Although the residual levels at both sites were lower than the maximum residue limit (MRL=1 mg/kg), the dissipation at Site 2 was faster than that at Site 1. Consequently, the half-life (t1/2) in Site 2 (5.2 d) was shorter than that in Site 1 (9.8 d). Risk assessment at zero days showed acceptable daily intakes (%) of 27.25% and 24.52% at Sites 1 and 2, respectively, indicating that these fruit species are safe for consumption.