Sadrettin Pence1, Ibrahim Ikizceli2, Emin Ozbek3, Necip Ozan Tiryakioglu1,4, Hilal Eren1,5,6, Emre Can Polat7, Halime Hanım Pence8. 1. Department of Molecular Medicine, Institute for Experimental Medical Research, Istanbul University, Istanbul, Turkey. 2. Department of Emergency Medicine, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey. 3. Department of Urology, Okmeydani Training and Research Hospital, Halic University, Istanbul, Turkey. 4. Department of Molecular Biology and Genetics, Halic University, Istanbul, Turkey. 5. Department of Histology and Embryology, Faculty of Medicine, Medipol University, Istanbul, Turkey. 6. REMER, Regenerative and Restorative Medicine Research Center, Medipol University, Istanbul, Turkey. 7. Department of Urology, Balikligol State Hospital, Sanliurfa, Istanbul, Turkey. 8. Zeytinburnu District Directorate of Health, Medipol University, Istanbul, Turkey.
Abstract
INTRODUCTION: Lower bone mineral density (BMD) and reduced Oxalobacter formigenes colonization are common findings in urolithiasis patients. But none of the studies conducted investigated the relationship between decreased bone mineral density and reduced Oxalobacter colonization. Here we evaluated the relation between BMD and O. formigenes colonization in urolithiasis patients. MATERIALS AND METHODS: 50 stone formers (48.9 ± 11.9 years) and 50 control (47.2 ± 13.4 years) adult male subjects were included in the study. Alterations in O. formigenes colonization were determined as absolute O. formigenes count from fecal samples by real time polymerase chain reaction using species specific primers. BMD was evaluated from t- and z- scores calculated by using dual energy absorptiometry in the total femoral neck and lumbar spine (L2-L4). RESULTS: Low BMD was observed in 18 (36%) urinary stone forming patients and in 7 (14%) control subjects in the lumbar area (p < 0.05). The mean O. formigenes count in stone formers and control subjects were 19,257 (5,791 ± 1,117.93) and 143,850 (2,815,725 ± 3,946,044.7) (p < 0.05) respectively. We observed a correlation between decreased lumbar BMD and O. formigenes colonization and testosterone levels in stone formers. Our results indicated that diminished O. formigenes colonization in the gut of urinary stone forming subjects was associated with reduced BMD.
INTRODUCTION: Lower bone mineral density (BMD) and reduced Oxalobacter formigenes colonization are common findings in urolithiasis patients. But none of the studies conducted investigated the relationship between decreased bone mineral density and reduced Oxalobacter colonization. Here we evaluated the relation between BMD and O. formigenes colonization in urolithiasis patients. MATERIALS AND METHODS: 50 stone formers (48.9 ± 11.9 years) and 50 control (47.2 ± 13.4 years) adult male subjects were included in the study. Alterations in O. formigenes colonization were determined as absolute O. formigenes count from fecal samples by real time polymerase chain reaction using species specific primers. BMD was evaluated from t- and z- scores calculated by using dual energy absorptiometry in the total femoral neck and lumbar spine (L2-L4). RESULTS: Low BMD was observed in 18 (36%) urinary stone forming patients and in 7 (14%) control subjects in the lumbar area (p < 0.05). The mean O. formigenes count in stone formers and control subjects were 19,257 (5,791 ± 1,117.93) and 143,850 (2,815,725 ± 3,946,044.7) (p < 0.05) respectively. We observed a correlation between decreased lumbar BMD and O. formigenes colonization and testosterone levels in stone formers. Our results indicated that diminished O. formigenes colonization in the gut of urinary stone forming subjects was associated with reduced BMD.
Entities:
Keywords:
Bone mineral density; Oxalate degradation; Oxalobacter formigenes; Urolithiasis
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