Anselm G M Jünemann1, Bernhard Michalke2, Marianna Lucio3, Anwar Chaudhri4, Ursula Schlötzer-Schrehardt5, Robert Rejdak6, Marek Rękas7, Bettina Hohberger8. 1. Department of Ophthalmology, University of Rostock, Doberaner Straße 140, 18057 Rostock, Germany. Electronic address: anselm.juenemann@med.uni-rostock.de. 2. Helmholtz Zentrum München - German Research Center for Environmental Health, Research Unit Analytical BioGeoChemistry, Ingolstaedter Landstr. 1, 85764 Neuherberg, Germany. Electronic address: bernhard.michalke@helmholtz-muenchen.de. 3. Helmholtz Zentrum München - German Research Center for Environmental Health, Research Unit Analytical BioGeoChemistry, Ingolstaedter Landstr. 1, 85764 Neuherberg, Germany. Electronic address: marianna.lucio@helmholtz-muenchen.de. 4. Institute of Surface Science and Corrosion, Department of Material Science, Friedrich-Alexander-University of Erlangen-Nürnberg, Martensstraße 7, 91058 Erlangen, Germany. 5. Schwabachanlage 6, 91054 Erlangen, Germany. Electronic address: Ursula.Schloetzer-Schrehardt@uk-erlangen.de. 6. Medical University in Lublin, Aleje Racławickie 1, 20-059 Lublin, Poland. Electronic address: robertrejdak@yahoo.com. 7. Department of Ophthalmology, Military Institute of Medicine, Szaserów 128, 00-001 Warszawa, Poland. Electronic address: rekaspl@gmail.com. 8. Schwabachanlage 6, 91054 Erlangen, Germany. Electronic address: Bettina.hohberger@uk-erlangen.de.
Abstract
PURPOSE: Selenium supplementation was seen to be linked to glaucoma disease in a previous study (Lillico A. JE, Reid M et al. (2002) Selenium Supplementation and Risk of Glaucoma in the NPC trial University of Arizona, Tucson, AZ., Arizona Cancer Center). As aqueous humor levels of selenium seemed to be associated with primary open-angle glaucoma (POAG), the aim of this study was to analyze concentrations of selenium in aqueous humor samples of patients with POAG and pseudoexfoliation glaucoma (PEXG) in comparison to normal samples. PATIENTS AND METHODS: Thirty-eight aqueous humor samples from patients undergoing cataract surgery were collected: Eleven patients with PEXG (age 65.8 ± 10.69, female 6, male 5), 12 patients with POAG (age 65.3 ± 10.50, female 7, male 5) and 15 patients without glaucoma (age 70.9 ± 12.83, female 10, male 5, controls). Aqueous humor levels of selenium were measured by Flow-Injection-Inductively-Coupled-Plasma-DRC-Mass-Spectrometry (ICP-DRC-MS). RESULTS: Maximum likelihood estimation of the least squares means (LS-means) and the relative 95% confidence limits of selenium aqueous humor levels were 6.90 ± 1.03 μg/L (control), 6.74 ± 1.14 μg/L (POAG) and 8.25 ± 1.18 μg/L (PEXG). The data were modeled using a generalized linear model (GLM) analysis, where selenium was set as dependent variable. The model was corrected for group differences in age and gender. The data show no differences among all the calculated differences between the least square means (LS means), taking in consideration the simultaneous 95% confidence limit and the multiple comparison tests with Tukey-Cramer adjustment. The evaluation of the model disclosed that POAG and PEXG patients had no significantly different aqueous humor selenium concentrations compared to controls and to each other. However, the quantile regression analysis of selenium aqueous humor levels showed differences in quantiles for open-angle glaucoma patients considering age and gender. CONCLUSION: As no significant difference in aqueous humor concentration of selenium was detected between open-angle glaucoma and controls, however, quantile analysis showed differences in quantiles levels for different age ranges in open-angle glaucoma patients, the trace element selenium seemed to be linked to glaucoma disease, yet not in a major role.
PURPOSE:Selenium supplementation was seen to be linked to glaucoma disease in a previous study (Lillico A. JE, Reid M et al. (2002) Selenium Supplementation and Risk of Glaucoma in the NPC trial University of Arizona, Tucson, AZ., Arizona Cancer Center). As aqueous humor levels of selenium seemed to be associated with primary open-angle glaucoma (POAG), the aim of this study was to analyze concentrations of selenium in aqueous humor samples of patients with POAG and pseudoexfoliation glaucoma (PEXG) in comparison to normal samples. PATIENTS AND METHODS: Thirty-eight aqueous humor samples from patients undergoing cataract surgery were collected: Eleven patients with PEXG (age 65.8 ± 10.69, female 6, male 5), 12 patients with POAG (age 65.3 ± 10.50, female 7, male 5) and 15 patients without glaucoma (age 70.9 ± 12.83, female 10, male 5, controls). Aqueous humor levels of selenium were measured by Flow-Injection-Inductively-Coupled-Plasma-DRC-Mass-Spectrometry (ICP-DRC-MS). RESULTS: Maximum likelihood estimation of the least squares means (LS-means) and the relative 95% confidence limits of selenium aqueous humor levels were 6.90 ± 1.03 μg/L (control), 6.74 ± 1.14 μg/L (POAG) and 8.25 ± 1.18 μg/L (PEXG). The data were modeled using a generalized linear model (GLM) analysis, where selenium was set as dependent variable. The model was corrected for group differences in age and gender. The data show no differences among all the calculated differences between the least square means (LS means), taking in consideration the simultaneous 95% confidence limit and the multiple comparison tests with Tukey-Cramer adjustment. The evaluation of the model disclosed that POAG and PEXG patients had no significantly different aqueous humor selenium concentrations compared to controls and to each other. However, the quantile regression analysis of selenium aqueous humor levels showed differences in quantiles for open-angle glaucomapatients considering age and gender. CONCLUSION: As no significant difference in aqueous humor concentration of selenium was detected between open-angle glaucoma and controls, however, quantile analysis showed differences in quantiles levels for different age ranges in open-angle glaucomapatients, the trace element selenium seemed to be linked to glaucoma disease, yet not in a major role.