Michał Post1, Wojciech Lubiński2, Jan Lubiński3, Karol Krzystolik4, Piotr Baszuk5, Magdalena Muszyńska6, Wojciech Marciniak7. 1. 2nd Department of Ophthalmology, Pomeranian Medical University, Szczecin, Poland. michalpost.md@gmail.com. 2. 2nd Department of Ophthalmology, Pomeranian Medical University, Szczecin, Poland. lubinski@pro.onet.pl. 3. Department of Genetics and Pathomorphology, Pomeranian Medical University, Szczecin, Poland. lubinski@pum.edu.pl. 4. 1st Department of Ophthalmology, Pomeranian Medical University, Szczecin, Poland. krzystolikk@poczta.onet.pl. 5. Department of Genetics and Pathomorphology, Pomeranian Medical University, Szczecin, Poland. baszukpiotr@gmail.com. 6. Department of Genetics and Pathomorphology, Pomeranian Medical University, Szczecin, Poland. laboratory@read-gene.com. 7. Department of Genetics and Pathomorphology, Pomeranian Medical University, Szczecin, Poland. wojciech.marciniak90@gmail.com.
Abstract
OBJECTIVE: The aim of the study is to analyse correlations between age-related cataract (ARC), serum selenium levels and glutathione peroxidase gene 1 and 4 (GPX-1 and GPX-4). MATERIAL AND METHODS: A total sample of 275 participants were enrolled into the study: group A, 94 subjects elligible for ARC surgery, and group B, 181 volunteers without ocular symptoms, gender-, age-, and smoking- status and volume-matched at 1:2 with subjects in group A. All participants (n=275) were divided according to the Lens Opacities Classification System III (LOCS III) into: 1) study group (subjects with clinically significant cataract; N≥3 or C≥3 or P≥2), 2) control group (controls with clinically non-significant cataract; N<3 and C<3 and P<2). The single nucleotide polymorphisms of GPX-1 and GPX-4 were assessed using Real Time PCR. Serum selenium levels were assayed using Inductively Coupled Plasma Mass Spectrometry. RESULTS: Low selenium levels significantly predicted any age-related cataract (OR 7.969; p<.01), nuclear cataract (OR 12.823; p<.01) and cortical cataract (OR 3.31; p<.01). There was no significant effect of gender, age, SNP GPX-1 and SNP GPX-4 on the prevalence of age-related nuclear, cortical and posterior sub-capsular cataract. Serum selenium levels of 75-85 µg/L were associated with the lowest prevalence of ARC. CONCLUSIONS: Due to a confirmed association between serum selenium levels and age-related cataract, low serum selenium levels may constitute a potential risk factor of age-related cataract.
OBJECTIVE: The aim of the study is to analyse correlations between age-related cataract (ARC), serum selenium levels and glutathione peroxidase gene 1 and 4 (GPX-1 and GPX-4). MATERIAL AND METHODS: A total sample of 275 participants were enrolled into the study: group A, 94 subjects elligible for ARC surgery, and group B, 181 volunteers without ocular symptoms, gender-, age-, and smoking- status and volume-matched at 1:2 with subjects in group A. All participants (n=275) were divided according to the Lens Opacities Classification System III (LOCS III) into: 1) study group (subjects with clinically significant cataract; N≥3 or C≥3 or P≥2), 2) control group (controls with clinically non-significant cataract; N<3 and C<3 and P<2). The single nucleotide polymorphisms of GPX-1 and GPX-4 were assessed using Real Time PCR. Serum selenium levels were assayed using Inductively Coupled Plasma Mass Spectrometry. RESULTS: Low selenium levels significantly predicted any age-related cataract (OR 7.969; p<.01), nuclear cataract (OR 12.823; p<.01) and cortical cataract (OR 3.31; p<.01). There was no significant effect of gender, age, SNP GPX-1 and SNP GPX-4 on the prevalence of age-related nuclear, cortical and posterior sub-capsular cataract. Serum selenium levels of 75-85 µg/L were associated with the lowest prevalence of ARC. CONCLUSIONS: Due to a confirmed association between serum selenium levels and age-related cataract, low serum selenium levels may constitute a potential risk factor of age-related cataract.
Authors: Fotios S Fousekis; Andreas Katsanos; Konstantinos H Katsanos; Dimitrios K Christodoulou Journal: Int Ophthalmol Date: 2020-01-08 Impact factor: 2.031
Authors: Zuzanna Micun; Martyna Falkowska; Maryla Młynarczyk; Jan Kochanowicz; Katarzyna Socha; Joanna Konopińska Journal: Int J Environ Res Public Health Date: 2022-08-20 Impact factor: 4.614