Tobias Ladner1, Sebastian Odenwald1, Kevin Kerls1, Gerald Zieres1, Adeline Boillon2, Julien Bœuf3. 1. Roche Diagnostics Deutschland GmbH, Sandhofer Str. 116, 68305, Mannheim, Germany. 2. F. Hoffmann-La Roche Ltd., Grenzacherstrasse 124, 4070, Basel, Switzerland. 3. Roche Diagnostics Deutschland GmbH, Sandhofer Str. 116, 68305, Mannheim, Germany. julien.boeuf@roche.com.
Abstract
PURPOSE: Biological pharmaceutical unit operations like homogenization or pooling of liquids are often performed in stirred vessels. Bottom-mounted magnetic stirrers are usually the system of choice in drug product manufacturing, because bottom-mounted magnetic stirrers are considered to be gentle mixing systems. Nevertheless, magnetic stirrers can cause shear stress and, thus, lead to protein damage. METHODS: This study uses computational fluid dynamics (CFD), because flow and shear rates cannot easily be measured at the spot of interest. The investigation utilizes CFD models, which were checked for plausibility by comparing experimental results and model outcome. The investigators first modeled macroscopic flow across a range of vessel volume capacities. Subsequently, detailed models focusing on two locations (bearing gap (2 mm - 3.5 mm) and spigot gap (40 μm - 80 μm)) were developed. RESULTS: The macroscopic flow modeling showed that the direction of flow varies based on the vessel volume capacity. The detailed CFD model estimated significant flow through the bearing gap. However, the calculated shear rates in the bearing gap were always lower than the shear rates which occur directly next to the impeller tip. The CFD model calculated significantly higher shear rates in the spigot gap and flow in the lower microliter range. CONCLUSIONS: Shear rates at the impeller tip are typically used as parameter to characterize stirred mixing systems. Although higher shear rates were found in the spigot gap, these higher shear rates can most likely be neglected for most applications due to non-significant flow through the spigot gap.
PURPOSE: Biological pharmaceutical unit operations like homogenization or pooling of liquids are often performed in stirred vessels. Bottom-mounted magnetic stirrers are usually the system of choice in drug product manufacturing, because bottom-mounted magnetic stirrers are considered to be gentle mixing systems. Nevertheless, magnetic stirrers can cause shear stress and, thus, lead to protein damage. METHODS: This study uses computational fluid dynamics (CFD), because flow and shear rates cannot easily be measured at the spot of interest. The investigation utilizes CFD models, which were checked for plausibility by comparing experimental results and model outcome. The investigators first modeled macroscopic flow across a range of vessel volume capacities. Subsequently, detailed models focusing on two locations (bearing gap (2 mm - 3.5 mm) and spigot gap (40 μm - 80 μm)) were developed. RESULTS: The macroscopic flow modeling showed that the direction of flow varies based on the vessel volume capacity. The detailed CFD model estimated significant flow through the bearing gap. However, the calculated shear rates in the bearing gap were always lower than the shear rates which occur directly next to the impeller tip. The CFD model calculated significantly higher shear rates in the spigot gap and flow in the lower microliter range. CONCLUSIONS: Shear rates at the impeller tip are typically used as parameter to characterize stirred mixing systems. Although higher shear rates were found in the spigot gap, these higher shear rates can most likely be neglected for most applications due to non-significant flow through the spigot gap.
Entities:
Keywords:
CFD simulation; drug product; magnetic stirrer; monoclonal antibody; shear stress
Authors: Jared S Bee; Jennifer L Stevenson; Bhavya Mehta; Juraj Svitel; Joey Pollastrini; Robert Platz; Erwin Freund; John F Carpenter; Theodore W Randolph Journal: Biotechnol Bioeng Date: 2009-08-01 Impact factor: 4.530