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Literature DB >> 3025244

Two enzyme immunoassays for the detection of antibody to rodent coronaviruses.

Abstract

Two enzyme immunoassays for detection of antibody to rodent coronaviruses were compared. Mouse hepatitis virus (MHV), strain JHM, antigen was in the form of formalin-fixed, infected 17 C1 1 cells. This antigen detected antibody to the homologous strain of MHV as well as to two heterologous MHV strains and a serologically related rat coronavirus, sialodacryodenititis virus. Antibody titers in assays using horseradish peroxidase (HRP)-conjugated or ureiase-conjugated anti-mouse IgG were substantially higher than in an indirect immunofluorescence assay. The ureiase assay was somewhat more sensitive than the HRP assay. MHV-JHM antigen was stable under a variety of storage conditions for at least two months.

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Year: 1986 PMID： 3025244 PMCID： PMC7119637 DOI： 10.1016/0166-0934(86)90035-2

Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014

7 in total

1. Establishment of a clone of mouse liver cells from a single isolated cell.

Authors: G L HOBBS; K K SANFORD; V J EVANS; W R EARLE
Journal: J Natl Cancer Inst Date: 1957-05 Impact factor: 13.506

2. Enzyme-linked immunosorbent assay for detection of antibodies to murine hepatitis virus.

Authors: R L Peters; M J Collins; A J O'Beirne; P A Howton; S L Hourihan; S F Thomas
Journal: J Clin Microbiol Date: 1979-10 Impact factor: 5.948

3. Enhanced growth of a murine coronavirus in transformed mouse cells.

Authors: L S Sturman; K K Takemoto
Journal: Infect Immun Date: 1972-10 Impact factor: 3.441

4. Characterization of the virus of sialodacryoadenitis of rats: a member of the coronavirus group.

Authors: P N Bhatt; D H Percy; A M Jonas
Journal: J Infect Dis Date: 1972-08 Impact factor: 5.226

5. An immunofluorescence test for detection of serum antibody to rodent coronaviruses.

Authors: A L Smith
Journal: Lab Anim Sci Date: 1983-04

6. Replication of rat coronavirus in a rat cell line, LBC. Brief report.

Authors: N Hirano; K Ono; Y Sada; A Inoue; T Murakami; H Takamaru
Journal: Arch Virol Date: 1985 Impact factor: 2.574

7. Peritoneal macrophage alterations caused by naturally occurring mouse hepatitis virus.

Authors: G A Boorman; M I Luster; J H Dean; M L Campbell; L A Lauer; F A Talley; R E Wilson; M J Collins
Journal: Am J Pathol Date: 1982-01 Impact factor: 4.307

7 in total

10 in total

6. A comparison of the sensitivity and specificity of sialodacryoadenitis virus, Parker's rat coronavirus, and mouse hepatitis virus-infected cells as a source of antigen for the detection of antibody to rat coronaviruses.

Authors: D H Percy; K L Williams; F X Paturzo
Journal: Arch Virol Date: 1991 Impact factor: 2.574

Two enzyme immunoassays for the detection of antibody to rodent coronaviruses.

1. Establishment of a clone of mouse liver cells from a single isolated cell.

2. Enzyme-linked immunosorbent assay for detection of antibodies to murine hepatitis virus.

3. Enhanced growth of a murine coronavirus in transformed mouse cells.

4. Characterization of the virus of sialodacryoadenitis of rats: a member of the coronavirus group.

5. An immunofluorescence test for detection of serum antibody to rodent coronaviruses.

6. Replication of rat coronavirus in a rat cell line, LBC. Brief report.

7. Peritoneal macrophage alterations caused by naturally occurring mouse hepatitis virus.

1. Virus strain specificity of challenge immunity to coronavirus.

2. Mouse hepatitis virus and host determinants of vertical transmission and maternally-derived passive immunity in mice.

3. Duration of challenge immunity to coronavirus JHM in mice.

4. Responses of mice to murine coronavirus immunization.

5. Passively acquired challenge immunity to enterotropic coronavirus in mice.

6. A comparison of the sensitivity and specificity of sialodacryoadenitis virus, Parker's rat coronavirus, and mouse hepatitis virus-infected cells as a source of antigen for the detection of antibody to rat coronaviruses.

7. Maternally-derived passive immunity to enterotropic mouse hepatitis virus.

8. Response of genetically susceptible and resistant mice to intranasal inoculation with mouse hepatitis virus JHM.

9. An ELISA system for evaluating antiretroviral activity against Rauscher murine leukemia virus.

10. Viremic dissemination of mouse hepatitis virus-JHM following intranasal inoculation of mice.