Puja Kumari1, Pritam Kumar Panda2, Ealisha Jha3, Nandini Pramanik1, Kumari Nisha1, Khushboo Kumari1, Nikita Soni4, M Anwar Mallick1, Suresh K Verma5. 1. Advance Science & Technology Research Centre, Vinoba Bhave University, Hazaribagh, Jharkhand, 825301, India. 2. Division of Pediatric Hematology & Oncology, University Children's Hospital, University of Freiburg, 79106, Germany. 3. Department of Physics & Physical Oceanography, Memorial University of Newfoundland, St. John's, Newfoundland & Labrador, NL A1C 5S7 Canada. 4. School of Biotechnology & Bioinformatics, D. Y. Patil (deemed to be university), Navi Mumbai, India. 5. School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
Abstract
AIM: To investigate the biocompatibility of green synthesized copper oxide nanoparticles (CuO Np) using floral extract of Calotropis gigantea in room condition. MATERIALS & METHODS: Green synthesized and characterized CuO Np was evaluated for their cellular and molecular biocompatibility by experimentally and computational molecular docking. RESULTS: Synthesized CuO NP was found to have a size 32 ± 09 nm with ζ potential -35 ± 12 mV. LC50 value was found to be 190 μg/ml. In vitro and in silico cytotoxicity analysis with HEK293 cells revealed the cytotoxic effect of CuO Np as consequences of interaction with histidine and arginine amino acid residues of Sod3 and p53 proteins via hydrogen bond of length 3.09 and 3.32 Å leading to oxidative stress ensuing toward apoptosis and cell cycle arrest. CONCLUSION: The outcomes proved the synthesized material as an alternative to the conventional method of synthesizing copper nanoparticles for biomedical and clinical applications.
AIM: To investigate the biocompatibility of green synthesized copper oxide nanoparticles (CuO Np) using floral extract of Calotropis gigantea in room condition. MATERIALS & METHODS: Green synthesized and characterized CuO Np was evaluated for their cellular and molecular biocompatibility by experimentally and computational molecular docking. RESULTS: Synthesized CuO NP was found to have a size 32 ± 09 nm with ζ potential -35 ± 12 mV. LC50 value was found to be 190 μg/ml. In vitro and in silico cytotoxicity analysis with HEK293 cells revealed the cytotoxic effect of CuO Np as consequences of interaction with histidine and arginine amino acid residues of Sod3 and p53 proteins via hydrogen bond of length 3.09 and 3.32 Å leading to oxidative stress ensuing toward apoptosis and cell cycle arrest. CONCLUSION: The outcomes proved the synthesized material as an alternative to the conventional method of synthesizing copper nanoparticles for biomedical and clinical applications.
Entities:
Keywords:
Calotropis gigantea; CuO nanoparticles; HEK293; biocompatibility; human embryonic kidney cells line
Authors: Lina A Al-Ani; Wageeh A Yehye; Farkaad A Kadir; Najihah M Hashim; Mohammed A AlSaadi; Nurhidayatullaili M Julkapli; Vincent K S Hsiao Journal: PLoS One Date: 2019-05-14 Impact factor: 3.240