Jesús Sanchis-Chordà1, Eva M Gómez Del Pulgar2, Joaquín Carrasco-Luna1,3, Alfonso Benítez-Páez2, Yolanda Sanz2, Pilar Codoñer-Franch4,5. 1. Department of Pediatrics, Obstetrics and Gynecology, University of Valencia, Avenida de Blasco Ibañez, no 15, 46010, Valencia, Spain. 2. Microbial Ecology, Nutrition and Health Research Group, Institute of Agrochemistry and Food Technology (IATA), National Research Council (CSIC), C/Médico Agustín Escardino, no 7, Paterna, 46980, Valencia, Spain. 3. Department of Experimental Sciences, Universidad Católica de Valencia, C/Quevedo, no 2, 46001, Valencia, Spain. 4. Department of Pediatrics, Obstetrics and Gynecology, University of Valencia, Avenida de Blasco Ibañez, no 15, 46010, Valencia, Spain. pilar.codoner@uv.es. 5. Department of Pediatrics, Dr. Peset University Hospital, Avenida Gaspar Aguilar, no 90, 46017, Valencia, Spain. pilar.codoner@uv.es.
Abstract
PURPOSE: The relationships between gut microbiota and obesity-related co-morbidities have been increasingly recognized. Low-grade inflammation may be the main factor in the pathogenesis of such disorders. We investigated the effect of the potential probiotic Bifidobacterium pseudocatenulatum CECT 7765 on cardiometabolic risk factors, inflammatory cytokines and gut microbiota composition in obese children with insulin resistance. METHODS: The study included 48 obese children (10-15 years old) with insulin resistance. They received dietary advice and were assigned to take the capsules with or without probiotic (109-10 CFU) daily for 13 weeks. Clinical, biochemical and gut microbiome measurements were made at baseline and at the end of the intervention. RESULTS: There was a significant improvement in body mass index in all children after the intervention, suggesting that weight changes are related to the dietary advice. A significant decrease in circulating high-sensitive C-reactive protein (P = 0.026) and monocyte chemoattractant protein-1 (P = 0.032) and an increase in high-density lipoprotein cholesterol (P = 0.035) and omentin-1 (P = 0.023) in children receiving probiotic supplementation were observed compared to the control group. Regarding gut microbiota, probiotic administration significantly increased the proportion of the Rikenellaceae family members, particularly of the Alistipes genus. CONCLUSIONS: The beneficial effects of the intervention on inflammatory markers and lipid profile suggest that B. pseudocatenulatum CECT 7765 intake together with dietary recommendations can improve inflammatory status in children with obesity and insulin resistance. These effects are parallel to increases in bacterial groups associated with a lean phenotype. The modulation of gut microbiota with probiotic supplementation can be considered an effective tool to ameliorate some obesity-related disorders in children.
RCT Entities:
PURPOSE: The relationships between gut microbiota and obesity-related co-morbidities have been increasingly recognized. Low-grade inflammation may be the main factor in the pathogenesis of such disorders. We investigated the effect of the potential probiotic Bifidobacterium pseudocatenulatum CECT 7765 on cardiometabolic risk factors, inflammatory cytokines and gut microbiota composition in obesechildren with insulin resistance. METHODS: The study included 48 obesechildren (10-15 years old) with insulin resistance. They received dietary advice and were assigned to take the capsules with or without probiotic (109-10 CFU) daily for 13 weeks. Clinical, biochemical and gut microbiome measurements were made at baseline and at the end of the intervention. RESULTS: There was a significant improvement in body mass index in all children after the intervention, suggesting that weight changes are related to the dietary advice. A significant decrease in circulating high-sensitive C-reactive protein (P = 0.026) and monocyte chemoattractant protein-1 (P = 0.032) and an increase in high-density lipoprotein cholesterol (P = 0.035) and omentin-1 (P = 0.023) in children receiving probiotic supplementation were observed compared to the control group. Regarding gut microbiota, probiotic administration significantly increased the proportion of the Rikenellaceae family members, particularly of the Alistipes genus. CONCLUSIONS: The beneficial effects of the intervention on inflammatory markers and lipid profile suggest that B. pseudocatenulatum CECT 7765 intake together with dietary recommendations can improve inflammatory status in children with obesity and insulin resistance. These effects are parallel to increases in bacterial groups associated with a lean phenotype. The modulation of gut microbiota with probiotic supplementation can be considered an effective tool to ameliorate some obesity-related disorders in children.
Entities:
Keywords:
Bifidobacterium pseudocatenulatum; Children obesity; Gut microbiota; Monocyte chemoattractant protein-1; Omentin-1; Probiotics
Authors: Annefleur M Koopen; Eduardo L Almeida; Ilias Attaye; Julia J Witjes; Elena Rampanelli; Soumia Majait; Marleen Kemper; Johannes H M Levels; Alinda W M Schimmel; Hilde Herrema; Torsten P M Scheithauer; Werner Frei; Lars Dragsted; Bolette Hartmann; Jens J Holst; Paul W O'Toole; Albert K Groen; Max Nieuwdorp Journal: Front Microbiol Date: 2021-06-10 Impact factor: 5.640