Presence of complement-dependent cytotoxic activity against clonally-derived rat islet tumour cells in sera from type 1 (insulin-dependent) diabetic patients and control subjects.

Heat-inactivated sera from newly diagnosed Type 1 (insulin-dependent) diabetic patients and control subjects were tested for the presence of antibodies to islet cell surface antigens by means of a sensitive immunofluorescent, microcytotoxicity assay using two clones of a rat islet cell tumour as antigens. Complement-dependent cytotoxicity was found in 74% of diabetic patient sera and 87% of control sera, and there were no significant differences in titres between diabetic patients and control subjects. A minority of the sera from both patients and controls were cytotoxic for only one of the two clones, suggesting the presence of multiple antigen-antibody systems. Preadsorptions of the sera with rat liver powder, sheep erythrocytes, and/or protein A-conjugated agarose beads were inconsistently effective in decreasing levels of lytic activity in control sera. It is concluded that more information is required concerning the antigens of rat islet cells and islet cell cytotoxic factors present in normal sera before such cells and assays can be reliably used for the detection of islet cell surface antibodies.