| Literature DB >> 30247470 |
Allyson L Huttinger1, Debra G Wheeler1, Surya Gnyawali2, David Dornbos1, Juliana M Layzer3, Nicholas Venetos1, Spencer Talentino1, Nicholas J Musgrave1, Cheyenne Jones1, Camille Bratton1, Matthew E Joseph4, Chandan Sen2, Bruce A Sullenger3, Shahid M Nimjee5.
Abstract
Occlusive arterial thrombosis leading to cerebral ischemic stroke and myocardial infarction contributes to ~13 million deaths every year globally. Here, we have translated a vascular injury model from a small animal into a large animal (canine), with slight modifications that can be used for pre-clinical screening of prophylactic and thrombolytic agents. In addition to the surgical methods, the modified protocol describes the step-by-step methods to assess carotid artery canalization by angiography, detailed instructions to process both the brain and carotid artery for histological analysis to verify carotid canalization and cerebral hemorrhage, and specific parameters to complete an assessment of downstream thromboembolic events by utilizing magnetic resonance imaging (MRI). In addition, specific procedural changes from the previously well-established small animal model necessary to translate into a large animal (canine) vascular injury are discussed.Entities:
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Year: 2018 PMID： 30247470 PMCID： PMC6235140 DOI： 10.3791/57981
Source DB: PubMed Journal: J Vis Exp ISSN： 1940-087X Impact factor: 1.355