Role of AgNPs in the enhancement of seed germination and its effect on plumule and radicle length of Pennisetum glaucum.

The authors have investigated beneficial effects of 1 mM of silver nanoparticles (AgNPs) on agriculturally important plant Pennisetum glaucum (Bajara). The extracellular AgNPs were synthesised using Bacillus subtilis spizizenni and characterised using ultraviolet-visible absorption spectroscopy, Fourier transform infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM). Optical absorption spectrum showed characteristic peak of AgNPs at 423 nm. FT-IR analysis of AgNPs showed peak at 3435 cm-1, which indicates the presence of N-H group (primary, secondary amines and amides) on the surface of AgNPs. TEM studies indicate that synthesised AgNPs have average size of ∼2 nm. Energy dispersive X-ray spectroscopy showed strong signal of Ag at 3 keV. Treatment of 1 mM AgNPs to the bajara seeds was found to be sufficient for excellent germination of seeds within 3 days. There was also significant increase in radicle and plumule length as compared with control bajara seeds according to statistical analysis by one-way analysis of variance, followed by Tukey's test. The percentage of AgNPs detected in root samples was 0.003% (by inductively coupled plasma atomic emission spectroscopy), which is negligible. There is still need to study the bioavailability and the type of interaction of AgNPs with plants, necessary for application in agriculture.