A novel fluorescent immunochromatographic strip combined with pocket fluorescence observation instrument for rapid detection of PRV.

Pseudorabies virus (PRV) is an acute and thermal infectious disease in domestic animals. Pigs are a main source of PRV infection, which causes high mortality rates for newborn infected piglets and high miscarriage rates for infected adults. Therefore, early control of PRV is necessary to avoid significant economic loss. We have developed a novel fluorescent immunochromatographic strip (F-ICS) for rapid, sensitive, and specific detection of PRV with a limit of detection (LOD) of 0.13 ng mL-1 and a detection linear range (DLR) between 0.13 and 2.13 ng mL-1. The detection limit was about 10 times lower than the colloidal gold strip. In tests of clinical samples, the F-ICS was largely consistent with PCR results, indicating its practical clinical application. In addition, for easy observation of the F-ICS signal by eye, we present a matching 3D-printed pocket fluorescence observation instrument (PFOI) that allows for use of the F-ICS in the field as easily as conventional colloidal gold strips. Graphical Abstract ᅟ.