Literature DB >> 3024536

Detection of transmissible gastroenteritis coronavirus antigens by a sandwich enzyme-linked immunosorbent assay technique.

S Bernard, I Lantier, H Laude, J M Aynaud.   

Abstract

A new sandwich enzyme-linked immunosorbent assay, using monoclonal and polyclonal antibodies, was developed to detect transmissible gastroenteritis virus antigens from cell culture and from intestinal wash or feces obtained from experimentally infected pigs. This technique was shown to be suitable for the detection of virulent field strain unadapted to cell culture. Cross reactions had not been observed with other enteric pathogens, rotavirus, porcine epizootic diarrhea virus, and Escherichia coli.

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Year:  1986        PMID： 3024536

Source DB:  PubMed          Journal:  Am J Vet Res        ISSN： 0002-9645            Impact factor:   1.156


  10 in total

1.  Lactogenic immunity to transmissible gastroenteritis (TGE) of swine induced by the attenuated Nouzilly strain of TGE virus: passive protection of piglets and detection of serum and milk antibody classes by ELISA.

Authors:  S Bernard; J Shirai; I Lantier; E Bottreau; J M Aynaud
Journal:  Vet Immunol Immunopathol       Date:  1990-01       Impact factor: 2.046

2.  Detection of porcine respiratory coronavirus and transmissible gastroenteritis virus by an enzyme-linked immunosorbent assay.

Authors:  E Cornaglia; N Chrétien; S Charara; Y Elazhary
Journal:  Vet Microbiol       Date:  1994-12       Impact factor: 3.293

3.  Detection of transmissible gastroenteritis virus by RT-PCR and differentiation from porcine respiratory coronavirus.

Authors:  D Paton; G Ibata; J Sands; A McGoldrick
Journal:  J Virol Methods       Date:  1997-07       Impact factor: 2.014

4.  Kinetics of the in vitro antibody response to transmissible gastroenteritis (TGE) virus from pig mesenteric lymph node cells, using the ELISASPOT and ELISA tests.

Authors:  P Berthon; S Bernard; H Salmon; R M Binns
Journal:  J Immunol Methods       Date:  1990-08-07       Impact factor: 2.303

5.  Transmissible gastroenteritis coronavirus: surface antigens induced by virulent and attenuated strains.

Authors:  L T To; S Bernard; E Bottreau
Journal:  Res Virol       Date:  1992 Jul-Aug

6.  Humoral immune response to Salmonella abortusovis in sheep: in vitro induction of an antibody synthesis from either sensitized or unprimed lymph node cells.

Authors:  P Berthon; I Gohin; I Lantier; M Olivier
Journal:  Vet Immunol Immunopathol       Date:  1994-06       Impact factor: 2.046

7.  Induction of lactogenic immunity to transmissible gastroenteritis virus of swine using an attenuated coronavirus mutant able to survive in the physicochemical environment of the digestive tract.

Authors:  J M Aynaud; S Bernard; E Bottreau; I Lantier; H Salmon; P Vannier
Journal:  Vet Microbiol       Date:  1991-02-01       Impact factor: 3.293

8.  Étude comparée de trois souches du coronavirus de la gastroentérite transmissible: Conditions de la réplicationvirale et de la synthèse des antigènes structuraux.

Authors:  T D Nguyen; S Bernard; E Bottreau; I Lantier; J M Aynaud
Journal:  Ann Inst Pasteur Virol       Date:  2009-09-23

9.  Development of a novel real-time RT-PCR assay with LUX primer for the detection of swine transmissible gastroenteritis virus.

Authors:  Ru Chen; Weiming Huang; Zhixiong Lin; Zhongfang Zhou; Haiqiong Yu; Daozhong Zhu
Journal:  J Virol Methods       Date:  2004-12-01       Impact factor: 2.014

10.  Pathogenicity and antigen detection of the Nouzilly strain of transmissible gastroenteritis coronavirus, in 1-week-old piglets.

Authors:  M J Cubero; S Bernard; L Leon; P Berthon; A Contreras
Journal:  J Comp Pathol       Date:  1992-01       Impact factor: 1.311
  10 in total

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