Jordan S Taylor1, Jasmine Zeki1, Naohiko Ikegaki2, Liaohai L Chen3, Bill Chiu4. 1. Department of Surgery, Division of Pediatric Surgery, Stanford University School of Medicine, Stanford, CA. 2. Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, IL. 3. Department of Surgery, University of Illinois at Chicago, Chicago, IL. Electronic address: lhchen@uic.edu. 4. Department of Surgery, Division of Pediatric Surgery, Stanford University School of Medicine, Stanford, CA. Electronic address: bhsc@stanford.edu.
Abstract
PURPOSE: Precise excision of neuroblastoma is challenging, especially when tumors adhere to vital structures. Indocyanine green (ICG), an FDA-approved dye with absorption peaking at 800 nm, can absorb the near IR laser energy and release heat in the dyed tissue. We hypothesize that by injecting ICG at tumor sites followed by precise laser application, tumor cell death can be selectively targeted. METHODS: Orthotopic neuroblastoma tumors were created in the adrenal gland of immunocompromised mice. Tumor, liver, kidney, and muscle tissues were chosen for ICG injection. Intervention variables included presence of tumor capsule, continuous vs. pulsed laser treatment and total energy delivered. Control groups included laser or ICG only. Tissues were stained with hematoxylin/eosin. RESULTS: Continuous wave laser generated excessive heat, causing damage in all tissues. When using pulsed laser treatment, liver, kidney, muscle, and intact tumor tissues showed no cell death when treated with laser alone or laser plus ICG. Tumor tissue with the capsule removed, however, showed cell death on histology. CONCLUSIONS: Pulsed laser treatment combined with ICG causes targeted tumor cell death in neuroblastoma tumor without capsule. No cell death was observed when tumor capsule was present, when only laser was used, or when applied over non-tumor tissues.
PURPOSE: Precise excision of neuroblastoma is challenging, especially when tumors adhere to vital structures. Indocyanine green (ICG), an FDA-approved dye with absorption peaking at 800 nm, can absorb the near IR laser energy and release heat in the dyed tissue. We hypothesize that by injecting ICG at tumor sites followed by precise laser application, tumor cell death can be selectively targeted. METHODS: Orthotopic neuroblastoma tumors were created in the adrenal gland of immunocompromised mice. Tumor, liver, kidney, and muscle tissues were chosen for ICG injection. Intervention variables included presence of tumor capsule, continuous vs. pulsed laser treatment and total energy delivered. Control groups included laser or ICG only. Tissues were stained with hematoxylin/eosin. RESULTS: Continuous wave laser generated excessive heat, causing damage in all tissues. When using pulsed laser treatment, liver, kidney, muscle, and intact tumor tissues showed no cell death when treated with laser alone or laser plus ICG. Tumor tissue with the capsule removed, however, showed cell death on histology. CONCLUSIONS: Pulsed laser treatment combined with ICG causes targeted tumor cell death in neuroblastoma tumor without capsule. No cell death was observed when tumor capsule was present, when only laser was used, or when applied over non-tumor tissues.
Authors: Jeannine Coburn; Jamie Harris; Alexander D Zakharov; Jennifer Poirier; Naohiko Ikegaki; Andre Kajdacsy-Balla; Monika Pilichowska; Alexander V Lyubimov; Hiroyuki Shimada; David L Kaplan; Bill Chiu Journal: Int J Cancer Date: 2016-11-03 Impact factor: 7.396
Authors: Michael P La Quaglia; Brian H Kushner; Wendy Su; Glenn Heller; Kim Kramer; Sara Abramson; Nancy Rosen; Suzanne Wolden; Nai-Kong V Cheung Journal: J Pediatr Surg Date: 2004-03 Impact factor: 2.545