Literature DB >> 302438

Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA.

L I Grossman, R C Parker, R M Watson, S E Chandler, M Teplitz.   

Abstract

A putative HeLa cell culture line was discovered to be contaminated with mouse cells by examination of agarose gel profiles of restriction endonuclease digests of mitochondrial DNA. The contamination was confirmed by karyotypic analysis, and by observation of the mouse satellite band in an analytical buoyant density centrifugation of total cellular DNA. Restriction endonuclease analysis of mitochondrial DNA is suggested as a useful method for monitoring the species of cells in culture.

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Year:  1977        PMID： 302438      PMCID： PMC343754          DOI： 10.1093/nar/4.5.1267

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.

Authors:  M Thomas; R W Davis
Journal:  J Mol Biol       Date:  1975-01-25       Impact factor: 5.469

2.  A restriction endonuclease cleavage map of mouse mitochondrial DNA.

Authors:  K H Moore; P H Johnson; S E Chandler; L I Grossman
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

3.  The number of mitochondrial deoxyribonucleic acid genomes in mouse L and human HeLa cells. Quantitative isolation of mitochondrial deoxyribonucleic acid.

Authors:  D Bogenhagen; D A Clayton
Journal:  J Biol Chem       Date:  1974-12-25       Impact factor: 5.157

4.  Banded marker chromosomes as indicators of intraspecies cellular contamination.

Authors:  W A Nelson-Rees; R R Flandermeyer; P K Hawthorne
Journal:  Science       Date:  1974-06-07       Impact factor: 47.728

Review 5.  Genetic markers as tracers in cell culture.

Authors:  S M Gartler
Journal:  Natl Cancer Inst Monogr       Date:  1967-09

6.  Homology and structural relationships between the dimeric and monomeric circular forms of mitochondrial DNA from human leukemic leukocytes.

Authors:  D A Clayton; R W Davis; J Vinograd
Journal:  J Mol Biol       Date:  1970-01-28       Impact factor: 5.469

7.  Restriction endonuclease cleavage maps of rat and mouse mitochondrial DNAs.

Authors:  R C Parker; R M Watson
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

8.  Specific cleavage analysis of mammalian mitochondrial DNA.

Authors:  S S Potter; J E Newbold; C A Hutchison; M H Edgell
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205
  8 in total
  1 in total

1.  HeLa cell identification by analysis of ribosomal DNA segment patterns generated by endonuclease restriction.

Authors:  R D Schmickel; J R Waterson; M Knoller; L L Szura; G N Wilson
Journal:  Am J Hum Genet       Date:  1980-11       Impact factor: 11.025
  1 in total

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