Wenying Song1, Yanli Wang2, Qing Chu1, Congcong Qi3, Yuehua Gao3, Yan Gao1, Lili Xiang1, Xu Zhenzhen1, Yuguang Gao4. 1. Department of Stomatology, Hospital Affiliated to Binzhou Medical University, Binzhou City, Shandong Province, 256603, People's Republic of China. 2. Binzhou People's Hospital of Shandong Province, Shandong Binzhou 2566610, People's Republic of China. 3. Institute of Stomatology, Binzhou Medical University, Yantai, Shandong Province 264003, People's Republic of China. 4. Department of Stomatology, Hospital Affiliated to Binzhou Medical University, Binzhou City, Shandong Province, 256603, People's Republic of China. Electronic address: 865553126@qq.com.
Abstract
OBJECTIVES: In order to understand the specific in vivo function of transforming growth factor-beta1 (TGF-β1), we successfully established aTGF-β1 deficient mouse model using a conditional knockout method. In the present study, we aimed to further understand the potential role of TGF-β1 in enamel formation. DESIGN: Transgenic mice withoutTGF-β1 in epithelial cells were generated. Scanning electron microscopy and micro-computed tomography analysis were used to detect the dental appearance, enamel microstructure and tooth density. Histological analysis was used to examine the residual organic matrix of enamel. Quantitative real-time polymerase chain reaction was used to analyze the expressions of enamel matrix proteins at the mRNA level. RESULTS: The enamel of mandibular molars and incisors inTGF-β1 conditional knockout mice displayed severe attrition and lower density compared with the wild-type littermates. A slender microstructure of enamel rod was observed, and enamel matrix proteins were retained in the enamel space at the maturation stage in conditional knockout mice. Moreover, the expressions of enamel matrix protein-encoding genes, such as amelogenin (Amelx), ameloblastin (Ambn), Enamelin (Enam) and matrix metalloproteinase-20 (Mmp-20), were increased in enamel organs of conditional knockout mice. On the other hand, the expressions of Amelotin (Amtn), kallikrein-related peptidase-4 (Klk4), C4orf26 and WD repeat-containing protein 72 (Wdr72) were dramatically decreased at the transition and maturation stages. CONCLUSIONS: TGF-β1 played an important role in enamel mineralization through decreasing synthesis ofAmelx, Ambn and Enam and increasing synthesis of Klk4, Amtn, Corf26 and Wdr72.
OBJECTIVES: In order to understand the specific in vivo function of transforming growth factor-beta1 (TGF-β1), we successfully established aTGF-β1 deficient mouse model using a conditional knockout method. In the present study, we aimed to further understand the potential role of TGF-β1 in enamel formation. DESIGN:Transgenic mice withoutTGF-β1 in epithelial cells were generated. Scanning electron microscopy and micro-computed tomography analysis were used to detect the dental appearance, enamel microstructure and tooth density. Histological analysis was used to examine the residual organic matrix of enamel. Quantitative real-time polymerase chain reaction was used to analyze the expressions of enamel matrix proteins at the mRNA level. RESULTS: The enamel of mandibular molars and incisors inTGF-β1 conditional knockout mice displayed severe attrition and lower density compared with the wild-type littermates. A slender microstructure of enamel rod was observed, and enamel matrix proteins were retained in the enamel space at the maturation stage in conditional knockout mice. Moreover, the expressions of enamel matrix protein-encoding genes, such as amelogenin (Amelx), ameloblastin (Ambn), Enamelin (Enam) and matrix metalloproteinase-20 (Mmp-20), were increased in enamel organs of conditional knockout mice. On the other hand, the expressions of Amelotin (Amtn), kallikrein-related peptidase-4 (Klk4), C4orf26 and WD repeat-containing protein 72 (Wdr72) were dramatically decreased at the transition and maturation stages. CONCLUSIONS: TGF-β1 played an important role in enamel mineralization through decreasing synthesis ofAmelx, Ambn and Enam and increasing synthesis of Klk4, Amtn, Corf26 and Wdr72.
Authors: Jennifer Tsi Gerber; Katheleen Miranda Dos Santos; Bruna Karas Brum; Maria Fernanda Pivetta Petinati; Michelle Nascimento Meger; Delson João da Costa; Mohammed Elsalanty; Erika Calvano Küchler; Rafaela Scariot Journal: Clin Oral Investig Date: 2021-03-02 Impact factor: 3.573
Authors: Tilman Jobst-Schwan; Verena Klämbt; Maureen Tarsio; John F Heneghan; Amar J Majmundar; Shirlee Shril; Florian Buerger; Isabel Ottlewski; Boris E Shmukler; Rezan Topaloglu; Seema Hashmi; Farkhanda Hafeez; Francesco Emma; Marcella Greco; Guido F Laube; Hanan M Fathy; Martin Pohl; Jutta Gellermann; Danko Milosevic; Michelle A Baum; Shrikant Mane; Richard P Lifton; Patricia M Kane; Seth L Alper; Friedhelm Hildebrandt Journal: Kidney Int Date: 2019-10-22 Impact factor: 10.612