Ning Wang1, Xiaona Meng2, Yongyi Liu3, Yong Chen4, Qingwei Liang5. 1. Department of Orthopaedics, the First Affiliated Hospital of China Medical University, Liaoning Provincial Education Department, Shenyang 110001, China; Department of Orthopaedics, Center Hospital Affiliated to Shenyang Medical College, Shenyang 110024, China. 2. Department of Biochemistry and Molecular Biol, College of Basic Medical Science, China Medical University, Shenyang 110122, China. Electronic address: xnmeng@cmu.edu.cn. 3. Department of Orthopaedics, the First Affiliated Hospital of China Medical University, Liaoning Provincial Education Department, Shenyang 110001, China. 4. Department of Orthopaedics, Center Hospital Affiliated to Shenyang Medical College, Shenyang 110024, China. 5. Department of Orthopaedics, the First Affiliated Hospital of China Medical University, Liaoning Provincial Education Department, Shenyang 110001, China. Electronic address: lqwcmu@163.com.
Abstract
OBJECTIVES: Osteosarcoma is one of common malignant tumors worldwide in the metaphysis of teenagers. The role of lncRNAs in Osteosarcoma has become an emerging area of research. MATERIALS AND METHODS: Cell migration and invasion were analyzed in Osteosarcoma cell following knockdown or overexpression by transfection with small interfering RNA (siRNA) or treated with LPS. Western blotting and Real-time RT-PCR methods were used to analyze the effects of LPS on EMT. RESULTS: We discovered that LPS could regulate cell migration and invasion and promote EMT. At the same time, LPS could regulate the expression of TLR4 and HOTAIR. In addition, knockdown of the expression of TLR4 partially reverses the promotion of cell invasion induced by LPS. CONCLUSIONS: Our results indicated that LPS coordinate the Osteosarcoma through TLR4/HOTAIR.
OBJECTIVES:Osteosarcoma is one of common malignant tumors worldwide in the metaphysis of teenagers. The role of lncRNAs in Osteosarcoma has become an emerging area of research. MATERIALS AND METHODS: Cell migration and invasion were analyzed in Osteosarcoma cell following knockdown or overexpression by transfection with small interfering RNA (siRNA) or treated with LPS. Western blotting and Real-time RT-PCR methods were used to analyze the effects of LPS on EMT. RESULTS: We discovered that LPS could regulate cell migration and invasion and promote EMT. At the same time, LPS could regulate the expression of TLR4 and HOTAIR. In addition, knockdown of the expression of TLR4 partially reverses the promotion of cell invasion induced by LPS. CONCLUSIONS: Our results indicated that LPS coordinate the Osteosarcoma through TLR4/HOTAIR.