Literature DB >> 3023850

Extramitochondrial citrate synthase activity in bakers' yeast.

T M Rickey, A S Lewin.   

Abstract

We isolated the gene for citrate synthase (citrate oxaloacetate lyase; EC 4.1.3.7) from Saccharomyces cerevisiae and ablated it by inserting the yeast LEU2 gene within its reading frame. This revealed a second, nonmitochondrial citrate synthase. Like the mitochondrial enzyme, this enzyme was sensitive to glucose repression. It did not react with antibodies against mitochondrial citrate synthase. Haploid cells lacking a gene for mitochondrial citrate synthase grew somewhat slower than wild-type yeast cells, but exhibited no auxotrophic growth requirements.

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Year:  1986        PMID： 3023850      PMCID： PMC367537          DOI： 10.1128/mcb.6.2.488-493.1986

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  25 in total

1.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  Analysis of products of mitochondrial protein synthesis in yeast: genetic and biochemical aspects.

Authors:  M Douglas; D Finkelstein; R A Butow
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

3.  The synthesis of yeast matrix mitochondrial enzymes is regulated by different levels of mitochondrial function.

Authors:  J Satrustegui; A Machado
Journal:  Arch Biochem Biophys       Date:  1977-12       Impact factor: 4.013

4.  Intracellular localization of enzymes in yeast.

Authors:  P S Perlman; H R Mahler
Journal:  Arch Biochem Biophys       Date:  1970-01       Impact factor: 4.013

5.  Studies on the regulation and localization of the glyoxylate cycle enzymes in Saccharomyces cerevisiae.

Authors:  W Duntze; D Neumann; J M Gancedo; W Atzpodien; H Holzer
Journal:  Eur J Biochem       Date:  1969-08

6.  Efficient isolation of genes by using antibody probes.

Authors:  R A Young; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

7.  Submitochondrial localization, cell-free synthesis, and mitochondrial import of 2-isopropylmalate synthase of yeast.

Authors:  D M Hampsey; A S Lewin; G B Kohlhaw
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

8.  Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources.

Authors:  E S Polakis; W Bartley
Journal:  Biochem J       Date:  1965-10       Impact factor: 3.857

9.  Derepression of citrate synthase in Saccharomyces cerevisiae may occur at the level of transcription.

Authors:  M A Hoosein; A S Lewin
Journal:  Mol Cell Biol       Date:  1984-02       Impact factor: 4.272

10.  Import of proteins into mitochondria. Partial purification of a matrix-located protease involved in cleavage of mitochondrial precursor polypeptides.

Authors:  P C Böhni; G Daum; G Schatz
Journal:  J Biol Chem       Date:  1983-04-25       Impact factor: 5.157
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  14 in total

1.  Association of glyoxylate and beta-oxidation enzymes with peroxisomes of Saccharomyces cerevisiae.

Authors:  M T McCammon; M Veenhuis; S B Trapp; J M Goodman
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

2.  The CCAAT box-binding factor stimulates ammonium assimilation in Saccharomyces cerevisiae, defining a new cross-pathway regulation between nitrogen and carbon metabolisms.

Authors:  V D Dang; C Bohn; M Bolotin-Fukuhara; B Daignan-Fornier
Journal:  J Bacteriol       Date:  1996-04       Impact factor: 3.490

3.  Distinct upstream activation regions for glucose-repressed and derepressed expression of the yeast citrate synthase gene CIT1.

Authors:  M Rosenkrantz; C S Kell; E A Pennell; M Webster; L J Devenish
Journal:  Curr Genet       Date:  1994-03       Impact factor: 3.886

4.  Saccharomyces cerevisiae contains two functional citrate synthase genes.

Authors:  K S Kim; M S Rosenkrantz; L Guarente
Journal:  Mol Cell Biol       Date:  1986-06       Impact factor: 4.272

5.  Structure and regulation of KGD1, the structural gene for yeast alpha-ketoglutarate dehydrogenase.

Authors:  B Repetto; A Tzagoloff
Journal:  Mol Cell Biol       Date:  1989-06       Impact factor: 4.272

6.  Lack of mitochondrial citrate synthase discloses a new meiotic checkpoint in a strict aerobe.

Authors:  Gwenaël Ruprich-Robert; Denise Zickler; Véronique Berteaux-Lecellier; Christian Vélot; Marguerite Picard
Journal:  EMBO J       Date:  2002-12-02       Impact factor: 11.598

7.  DNA sequences in chromosomes II and VII code for pyruvate carboxylase isoenzymes in Saccharomyces cerevisiae: analysis of pyruvate carboxylase-deficient strains.

Authors:  R Stucka; S Dequin; J M Salmon; C Gancedo
Journal:  Mol Gen Genet       Date:  1991-10

8.  Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces cerevisiae are encoded by distinct homologous genes.

Authors:  M Rosenkrantz; T Alam; K S Kim; B J Clark; P A Srere; L P Guarente
Journal:  Mol Cell Biol       Date:  1986-12       Impact factor: 4.272

9.  Intramitochondrial functions regulate nonmitochondrial citrate synthase (CIT2) expression in Saccharomyces cerevisiae.

Authors:  X S Liao; W C Small; P A Srere; R A Butow
Journal:  Mol Cell Biol       Date:  1991-01       Impact factor: 4.272

10.  Molecular cloning of the yeast mitochondrial aconitase gene (ACO1) and evidence of a synergistic regulation of expression by glucose plus glutamate.

Authors:  S P Gangloff; D Marguet; G J Lauquin
Journal:  Mol Cell Biol       Date:  1990-07       Impact factor: 4.272
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