Femke Noorman1, John R Hess2. 1. Netherlands Ministry of Defense, Military Blood Bank, Leiden, the Netherlands. 2. Department of Laboratory Medicine, University of Washington, Seattle, Washington.
Abstract
BACKGROUND: Thromboelastography (TEG) is widely advocated as a rapid method for obtaining critical blood coagulation data to guide resuscitation, but the method suffers well-known limits in sensitivity, repeatability, and interpretability. STUDY DESIGN AND METHODS: Mixtures of fresh human blood components were prepared that represent the range of blood element concentrations seen in health and disease and after injury. These mixtures were tested in a TEG device after kaolin, tissue factor and phospholipid, or tissue factor and phospholipid with abciximab activation. The results were measured as reproducibility and nonlinear effects in regression analysis and evaluated for interpretability. RESULTS: Clot strength was associated with increased platelet (PLT) content and plasma fibrinogen concentration and content. Increasing hematocrit (Hct) reduced while increasing PLT or plasma concentration increased TEG clot strength. The abciximab dose used to block PLT activity did not fully inhibit the PLT contribution to clot strength. Clot strength is logarithmically correlated in the absence and linearly correlated to PLT concentration in the presence of abciximab. TEG clot strength with or without abciximab is dependent on Hct, PLT, and plasma (fibrinogen) concentrations in complex patterns. CONCLUSION: Interpretation of TEG variables is limited without knowledge of the concentration of the blood components present. When "normal" TEG values are known for a certain PLT-plasma-red blood cell concentration, the assay can be used to assess PLT and plasma function in coagulation. The TEG "functional fibrinogen" assay should be used only as a gross estimate of the fibrinogen concentration in whole blood.
BACKGROUND: Thromboelastography (TEG) is widely advocated as a rapid method for obtaining critical blood coagulation data to guide resuscitation, but the method suffers well-known limits in sensitivity, repeatability, and interpretability. STUDY DESIGN AND METHODS: Mixtures of fresh human blood components were prepared that represent the range of blood element concentrations seen in health and disease and after injury. These mixtures were tested in a TEG device after kaolin, tissue factor and phospholipid, or tissue factor and phospholipid with abciximab activation. The results were measured as reproducibility and nonlinear effects in regression analysis and evaluated for interpretability. RESULTS: Clot strength was associated with increased platelet (PLT) content and plasma fibrinogen concentration and content. Increasing hematocrit (Hct) reduced while increasing PLT or plasma concentration increased TEG clot strength. The abciximab dose used to block PLT activity did not fully inhibit the PLT contribution to clot strength. Clot strength is logarithmically correlated in the absence and linearly correlated to PLT concentration in the presence of abciximab. TEG clot strength with or without abciximab is dependent on Hct, PLT, and plasma (fibrinogen) concentrations in complex patterns. CONCLUSION: Interpretation of TEG variables is limited without knowledge of the concentration of the blood components present. When "normal" TEG values are known for a certain PLT-plasma-red blood cell concentration, the assay can be used to assess PLT and plasma function in coagulation. The TEG "functional fibrinogen" assay should be used only as a gross estimate of the fibrinogen concentration in whole blood.