Horseradish peroxidase histochemistry on mounted sections from embedded specimen: a simple method for serial reconstruction of neuronal projections.

A combination of two procedures, embedding of specimens and horseradish peroxidase (HRP) histochemistry on mounted serial sections, is proposed for three-dimensional reconstruction of neuronal projections. To clarify the intrinsic organization of the peripheral nervous system, this simple method is more adequate than previously used techniques.