Samira Helena João-Souza1, Alana Cristina Machado2, Raquel Marianna Lopes3, Denise Maria Zezell4, Tais Scaramucci5, Ana Cecilia Corrêa Aranha6. 1. Department of Restorative Dentistry, University of São Paulo, School of Dentistry, Av. Prof. Lineu Prestes 2227, Cidade Universitária, São Paulo, SP, 05508-000, Brazil. Electronic address: samira.joaodesouza@zmk.unibe.ch. 2. Department of Restorative Dentistry, University of São Paulo, School of Dentistry, Av. Prof. Lineu Prestes 2227, Cidade Universitária, São Paulo, SP, 05508-000, Brazil. Electronic address: alanaam@usp.br. 3. Department of Restorative Dentistry, University of São Paulo, School of Dentistry, Av. Prof. Lineu Prestes 2227, Cidade Universitária, São Paulo, SP, 05508-000, Brazil. Electronic address: raquel.lopes@usp.br. 4. Institute of Nuclear Research (IPEN), University of São Paulo - IPEN/CNEN, Av. Professor Lineu Prestes 2242, São Paulo, SP, Brazil. Electronic address: zezell@usp.br. 5. Department of Restorative Dentistry, University of São Paulo, School of Dentistry, Av. Prof. Lineu Prestes 2227, Cidade Universitária, São Paulo, SP, 05508-000, Brazil. Electronic address: tais.sca@usp.br. 6. Department of Restorative Dentistry, University of São Paulo, School of Dentistry, Av. Prof. Lineu Prestes 2227, Cidade Universitária, São Paulo, SP, 05508-000, Brazil. Electronic address: acca@usp.br.
Abstract
OBJECTIVE: To evaluate dentin permeability and tubule occlusion of in-office desensitizing treatments, and to analyze their resistance to erosive/abrasive challenges. DESIGN: Ninety-one 1mm-thick dentin discs were immersed in EDTA solution for 5 min. After analyzing the maximum dentin permeability, the specimens were randomly allocated into 7 experimental groups (n = 10): Control (no treatment); Er,Cr:YSGG laser; Nd:YAG laser; Gluma Desensitizer; Duraphat; Pro-Argin toothpaste; Calcium Sodium Phosphosilicate (CSP) paste. The post-treatment permeability was assessed and then the specimens were subjected to a 5-day erosion-abrasion cycling protocol: 4x/day of immersion in citric acid solution (5 min;0.3%), followed by exposure to clarified human saliva (60 min). After the first and last acid challenges, specimens were brushed for 15 s, with exposure to the toothpaste slurry for total time of 2 min. Dentin permeability was re-measured (post-cycling). Percentage of dentin permeability for each experimental time was calculated in relation to the maximum permeability (%Lp). Data were analyzed with 2-way repeated measures ANOVA and Tukey tests (α = 0.05). Surface modifications were analyzed by scanning electron microscopy. RESULTS: In both experimental time CSP paste and Gluma Desensitizer did not differ from each other (p = 0.0874), and were the only groups that presented significantly lower %Lp than the Control (p = 0.026 and p = 0.022, respectively). After treatment, they were able to reduce dentin permeability in 82% and 72%, respectively. The %Lp post-cycling was higher than post-treatment value for all groups (p = 0.008). Dentin permeability increased 21% for CSP paste and 12% for Gluma, but they remained significant different from Control. Deposits on the surface were observed for CSP paste; and for Gluma, tubule diameters were shown to be smaller. CONCLUSIONS: CSP paste and Gluma Desensitizer were the only treatments able to decrease dentin permeability post-treatment and to sustain low permeability post-cycling.
OBJECTIVE: To evaluate dentin permeability and tubule occlusion of in-office desensitizing treatments, and to analyze their resistance to erosive/abrasive challenges. DESIGN: Ninety-one 1mm-thick dentin discs were immersed in EDTA solution for 5 min. After analyzing the maximum dentin permeability, the specimens were randomly allocated into 7 experimental groups (n = 10): Control (no treatment); Er,Cr:YSGG laser; Nd:YAG laser; Gluma Desensitizer; Duraphat; Pro-Argin toothpaste; Calcium Sodium Phosphosilicate (CSP) paste. The post-treatment permeability was assessed and then the specimens were subjected to a 5-day erosion-abrasion cycling protocol: 4x/day of immersion in citric acid solution (5 min;0.3%), followed by exposure to clarified human saliva (60 min). After the first and last acid challenges, specimens were brushed for 15 s, with exposure to the toothpaste slurry for total time of 2 min. Dentin permeability was re-measured (post-cycling). Percentage of dentin permeability for each experimental time was calculated in relation to the maximum permeability (%Lp). Data were analyzed with 2-way repeated measures ANOVA and Tukey tests (α = 0.05). Surface modifications were analyzed by scanning electron microscopy. RESULTS: In both experimental time CSP paste and Gluma Desensitizer did not differ from each other (p = 0.0874), and were the only groups that presented significantly lower %Lp than the Control (p = 0.026 and p = 0.022, respectively). After treatment, they were able to reduce dentin permeability in 82% and 72%, respectively. The %Lp post-cycling was higher than post-treatment value for all groups (p = 0.008). Dentin permeability increased 21% for CSP paste and 12% for Gluma, but they remained significant different from Control. Deposits on the surface were observed for CSP paste; and for Gluma, tubule diameters were shown to be smaller. CONCLUSIONS:CSP paste and Gluma Desensitizer were the only treatments able to decrease dentin permeability post-treatment and to sustain low permeability post-cycling.