Andreas Neumayr1, Afona Chernet2, Véronique Sydow2, Kerstin Kling2, Esther Kuenzli2, Hanspeter Marti2, Daniel H Paris2, Beatrice Nickel2, Niklaus D Labhardt3. 1. Swiss Tropical and Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland. Electronic address: andreas.neumayr@swisstph.ch. 2. Swiss Tropical and Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland. 3. Swiss Tropical and Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland; Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.
Abstract
BACKGROUND: Point-of-care circulating cathodic antigen (POC-CCA) urine cassette testing has become a popular approach to screen for Schistosoma infection. Since the test is also increasingly used for following-up of treatment success, we assessed the assay's diagnostic accuracy after praziquantel treatment of S. mansoni infection among Eritrean refugees in Switzerland. METHODS: In our preceding study, 107 asymptomatic Eritrean refugees in Switzerland were screened for schistosomiasis by stool microscopy, serology, and POC-CCA urine testing. Individuals screened positive by any method were treated with praziquantel and invited for a follow-up visit, repeating the same diagnostic procedures one year after treatment. The POC-CCA baseline and follow-up results were analyzed against the 'baseline microscopy positive cases' (= the most reliably true positive cases) and the 'baseline microscopy plus serology negative cases at baseline and follow-up' (= the most reliably true negative cases). RESULTS: Complete diagnostic baseline and follow-up sampling was available from 48 participants. Compared to most reliably true positive cases at baseline, POC-CCA testing had a sensitivity of 90%. Compared to most reliably true negative cases, POC-CCA testing had a specificity of 73.9%. CONCLUSION: We conclude that the POC-CCA urine test is valuable for screening but its use is not suitable for routine follow-up after treatment.
BACKGROUND: Point-of-care circulating cathodic antigen (POC-CCA) urine cassette testing has become a popular approach to screen for Schistosoma infection. Since the test is also increasingly used for following-up of treatment success, we assessed the assay's diagnostic accuracy after praziquantel treatment of S. mansoni infection among Eritrean refugees in Switzerland. METHODS: In our preceding study, 107 asymptomatic Eritrean refugees in Switzerland were screened for schistosomiasis by stool microscopy, serology, and POC-CCA urine testing. Individuals screened positive by any method were treated with praziquantel and invited for a follow-up visit, repeating the same diagnostic procedures one year after treatment. The POC-CCA baseline and follow-up results were analyzed against the 'baseline microscopy positive cases' (= the most reliably true positive cases) and the 'baseline microscopy plus serology negative cases at baseline and follow-up' (= the most reliably true negative cases). RESULTS: Complete diagnostic baseline and follow-up sampling was available from 48 participants. Compared to most reliably true positive cases at baseline, POC-CCA testing had a sensitivity of 90%. Compared to most reliably true negative cases, POC-CCA testing had a specificity of 73.9%. CONCLUSION: We conclude that the POC-CCA urine test is valuable for screening but its use is not suitable for routine follow-up after treatment.
Authors: Francesca Tamarozzi; Tamara Ursini; Pytsje T Hoekstra; Ronaldo Silva; Cecilia Costa; Federico Gobbi; Gerardo B Monteiro; Leonardo Motta; Govert J van Dam; Paul L Corstjens; Lisette van Lieshout; Dora Buonfrate Journal: Parasit Vectors Date: 2021-03-09 Impact factor: 3.876
Authors: Anne Straily; Emmy A Kavere; Dollycate Wanja; Ryan E Wiegand; Susan P Montgomery; Alex Mwaki; Alie Eleveld; W Evan Secor; Maurice R Odiere Journal: Am J Trop Med Hyg Date: 2021-11-08 Impact factor: 3.707
Authors: Pytsje T Hoekstra; Afona Chernet; Claudia J de Dood; Eric A T Brienen; Paul L A M Corstjens; Niklaus D Labhardt; Beatrice Nickel; Linda Wammes; Govert J van Dam; Andreas Neumayr; Lisette van Lieshout Journal: Am J Trop Med Hyg Date: 2022-02-28 Impact factor: 2.345