Yue Wu1, Shujun Wang2, Lei Shang3, Haifeng Zhang4, Jianxiu Qin1, Yating Ren2, Tiantian Ye2. 1. Department of Pharmaceutics, School of Chinese Medicines, Shenyang Pharmaceutical University, Shenyang, PR China. 2. Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, PR China. 3. Department of Pharmaceutics, School of Pharmacy, Shenyang Medical College, Shenyang, PR China. 4. Department of Pharmacology, School of Life Science & Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, PR China.
Abstract
AIM: This study is aimed to evaluate borneol as a penetration enhancer to improve brain target of nanoliposome. MATERIALS & METHODS: Effects of borneol on pharmacokinetics, targeting efficiency, brain subareas distribution and neuron-targeting level and pathway were studied by fluorescence spectrophotometry and immunofluorescence. RESULTS: Borneol did not influence physicochemical property of doxorubicin hydrochloride nanoliposome (Dox-nanoLips). Co-administration of Dox-nanoLips with borneol elevated brain-target efficiency due to selective distribution increase in the cerebral cortex and hippocampus without difference in contralateral hemisphere. Borneol improved neuronal-targeting level of Dox-nanoLips in the cortex, CA3 and dentate gyrus regions via opening tight junctions of blood-brain barrier and then bypassing astrocyte. CONCLUSION: Borneol is potential to be a promising penetration enhancer for nanocarrier to target neurons.
AIM: This study is aimed to evaluate borneol as a penetration enhancer to improve brain target of nanoliposome. MATERIALS & METHODS: Effects of borneol on pharmacokinetics, targeting efficiency, brain subareas distribution and neuron-targeting level and pathway were studied by fluorescence spectrophotometry and immunofluorescence. RESULTS:Borneol did not influence physicochemical property of doxorubicin hydrochloride nanoliposome (Dox-nanoLips). Co-administration of Dox-nanoLips with borneol elevated brain-target efficiency due to selective distribution increase in the cerebral cortex and hippocampus without difference in contralateral hemisphere. Borneol improved neuronal-targeting level of Dox-nanoLips in the cortex, CA3 and dentate gyrus regions via opening tight junctions of blood-brain barrier and then bypassing astrocyte. CONCLUSION:Borneol is potential to be a promising penetration enhancer for nanocarrier to target neurons.