| Literature DB >> 30232777 |
Eric A Huebner1,2, Stéphane Budel2, Zhaoxin Jiang2, Takao Omura1, Tammy Szu-Yu Ho1, Lee Barrett1, Janie S Merkel3, Luis M Pereira4, Nick A Andrews1, Xingxing Wang2, Bhagat Singh1, Kush Kapur1, Michael Costigan1,4, Stephen M Strittmatter5, Clifford J Woolf6.
Abstract
Axotomy results in permanent loss of function after brain and spinal cord injuries due to the minimal regenerative propensity of the adult central nervous system (CNS). To identify pharmacological enhancers of axon regeneration, 960 compounds were screened for cortical neuron axonal regrowth using an in vitro cortical scrape assay. Diltiazem, verapamil, and bromopride were discovered to facilitate axon regeneration in rat cortical cultures, in the presence of chondroitin sulfate proteoglycans (CSPGs). Diltiazem, an L-type calcium channel blocker (L-CCB), also promotes axon outgrowth in adult primary mouse dorsal root ganglion (DRG) and induced human sensory (iSensory) neurons.Entities:
Keywords: Axon regeneration; Chondroitin sulfate proteoglycans; Cortical neurons; Diltiazem; Scrape assay; Sensory neurons
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Year: 2018 PMID： 30232777 PMCID： PMC6424671 DOI： 10.1007/s12035-018-1349-5
Source DB: PubMed Journal: Mol Neurobiol ISSN： 0893-7648 Impact factor: 5.590