Literature DB >> 3023182

Isolation and characterization of cDNA encoding rabbit reticulocyte 2,3-bisphosphoglycerate synthase.

S Yanagawa, K Hitomi, R Sasaki, H Chiba.   

Abstract

We have isolated and sequenced a cDNA clone containing the entire coding region of rabbit reticulocyte 2,3-bisphosphoglycerate (DPG) synthase. The cDNA was verified by translation of the hybridization-selected RNA and by demonstrating identity of the deduced amino acid (aa) sequence to the sequences of CNBr peptides of the purified enzyme. The aa sequence of the enzyme was homologous to the reported sequence of the human enzyme [Haggarty et al., EMBO J. 2 (1983) 1213-1220], especially in the N-terminal half (aa 1-142). Northern blot analysis of rabbit reticulocyte poly(A)+ RNA revealed a single species of mRNA with about 1700 nucleotides.

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Year:  1986        PMID： 3023182     DOI： 10.1016/0378-1119(86)90181-2

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  5 in total

1.  The role of the C-terminal region in phosphoglycerate mutase.

Authors:  R A Walter; J Nairn; D Duncan; N C Price; S M Kelly; D J Rigden; L A Fothergill-Gilmore
Journal:  Biochem J       Date:  1999-01-01       Impact factor: 3.857

2.  Evolution of a bifunctional enzyme: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.

Authors:  J F Bazan; R J Fletterick; S J Pilkis
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

3.  Phosphoglycerate mutase from Streptomyces coelicolor A3(2): purification and characterization of the enzyme and cloning and sequence analysis of the gene.

Authors:  P J White; J Nairn; N C Price; H G Nimmo; J R Coggins; I S Hunter
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

4.  Structure, expression and duplication of genes which encode phosphoglyceromutase of Drosophila melanogaster.

Authors:  P D Currie; D T Sullivan
Journal:  Genetics       Date:  1994-10       Impact factor: 4.562

5.  The amino acid sequence of the small monomeric phosphoglycerate mutase from the fission yeast Schizosaccharomyces pombe.

Authors:  J Nairn; N C Price; L A Fothergill-Gilmore; G E Walker; J E Fothergill; B Dunbar
Journal:  Biochem J       Date:  1994-02-01       Impact factor: 3.857
  5 in total

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