Rapidly screening of α-glucosidase inhibitors from Dioscorea opposita Thunb. peel based on rGO@Fe3O4 nanocomposites microreactor.

Present study aimed to immobilise the α-glucosidase on suitable supports to construct enzymatic microreactors and their subsequent applicability in efficient inhibitor screening from the Chinese Yam (Dioscorea opposita Thunb.) peel. A type of lamellar and porous composites (rGO@Fe3O4) were synthesised with a facile one-step solvothermal method and employed as carriers to construct enzymatic microreactors for screening α-glucosidase ligand from the Chinese Yam peel in league with the high performance liquid chromatography and mass spectrometry (HPLC-MS). The immobilisation amount of α-glucosidase on rGO@Fe3O4 under the optimised conditions was about 40 μg α-glucosidase/mg carriers. Furthermore, the binding capacities of screened inhibitors, 2,4-dimethoxy-6,7-dihydroxyphenanthrene and batatasin I, were 35.6 and 68.2%, respectively. Hence, considering their high screening efficiency and excellent magnetic separation ability, these as-prepared nanocomposite consisting of rGO and Fe3O4 may be potential supports for the enzyme (such as α-glucosidase) immobilisation for rapid α-glucosidase inhibitors screening from the diverse nature resources.

Introduction

Type 2 diabetes mellitus (T2DM), mainly manifested as disorder of glucose metabolism, obesity, etc. is a kind of multifactorial disease and chronic metabolic diseases that influenced by gene defect, surrounding environments and living ways with insulin resistance and hyposecretion of insulin. α-glucosidase, a group of membrane-bound enzyme in the intestinal epithelial cells, hydrolyzes the substrates (starch, sucrose, etc.) that contain glucosidic linkage to release the glucose and then leads to the postprandial blood glucose increasing effect, thus the reversible inhibition effect of α-glucosidase was one of the approaches currently used to improving the glucose metabolism imbalances and fat abnormalities conditions. Turkish reseachers identified that four medicinal herbs, concluding Urtica dioica, Taraxacum officinale, Viscum album, and Myrtus communis, showed high α-glucosidase inhibitor activity, the inhibitor activities different according to different α-glucosidase origins, and Myrtus communis (IC50 =38 μg/mL) could be developed as a new type of physiologically functional drink for lowing the blood glucose content. Matsumura et al. found that the water-soluble extract from leaves of Eugenia uniflora L. (Myrtaceae) could highly inhibited the α-glucosidase and proved by sucrose tolerance test (STT), glucose tolerance test (GTT) in mice experiments, α-glucosidase inhibition assay, and could provide some useful informations for the design of α-glucosidase ligand medicines. The crude extracts from fresh tuberous rhizomes of Dioscorea opposita Thunb. (Chinese Yam) were studied against yeast α-glucosidase that showed strong inhibitory activities and trans-N-p-coumaroyltyramine (IC50=0.40 μM) was isolated from the Dioscoreaceae family for the first time in our previous work.

Bioactivity-guided screening has been one of mainstream methods to discover active natural products. Two active components (6-O-(p-coumaroyl)-D-glucopyranoside, methyl 6-O-(p-coumaroyl)-β-D-galactopyranoside) were identified and purified from Tinta Cão grape pomace extract with bioactivity-guided fractionation methods that strongly inhibited intestinal α-glucosidase, and could be the potential development of a novel anti-hyperglycaemic drugs. Bioactivity- guided screening by free enzymes could supply the useful information whether the complex natural resources inhibited corresponding enzyme, but this method continued to have a serious problem, lack of inhibitors separation ability, so immobilising the enzymes on solid substrates (such as nanoparticle, nanocomposite, etc.) to construct enzyme microreactors was a effective method that contained the advantages of separation ability and the highly sensitivity of bioactivity-guided screening method. Human serum albumin (HSA) functionalised by magnetic nanoparticle (MNPs) to construct HSA-MNPs were employed to successfully separate three saponins (dioscin, gracillin, and pseudo-protodioscin) from D. nipponica extract and showed strongly affinity with HSA, and this work present a easy and effective method to separated inhibitors from complex medicinal plants. Lipase-Fe3O4 magnetic nanoparticle conjugates (LMNPs) were developed based on ligand fishing method and effectively supplied to isolate quercetin-3-O-β-D-arabinopyranosyl-(1 → 2)-β-D-galactopyranoside and quercetin-3-O-β-D-glucuronide from lotus (Nelumbo nucifera Gaertn.) leaf extract that widely used in China for weight-loss foods, and this method showed great power for rapidly lipase inhibitors screening from edible and medicinal plants. Tao et al. developed a method using α-glucosidase-functionalised magnetic bead by covalent linkage to screen the enzyme inhibitors from Morus alba extract and successfully separated two flavonoids (isoquercitrin and astragalin), and their work demonstrated that enzyme-functionalised magnetic beads method might be applicable for discovering the active compounds in complex medicinal plants.

This work was to develop a facile and effective method to synthesise biocompatible nanocomposite, and then employed them as solid substrates to immobilise the α-glucosidase for the construction of α-glucosidase microreactors for the screening experiments of α-glucosidase inhibitors from Chinese Yam (Dioscorea opposita Thunb.) peel. The free NH2 groups on the biocompatible nanocomposite were ideal reaction sites for the functionlisation of α-glucosidase with the typical glutaraldehyde (GA) activation process by the generation of Schiff base. The construction diagrammatic illustration was shown in Figure 1. The application schematic diagram of the α-glucosidase microreactors in this experiment was shown in Figure 2. The results of this work demonstrated that rGO@Fe3O4 biocompatible nanocomposite prepared by one-pot solvothermal method are promising supports to achieve higher immobilisation efficiency and binding capacities.

Figure 1.

The synthesis procedure of AMGO nanocomposites, and α-AMGO microreactors (A) and the application schematic diagram (B) of the α-glucosidase microreactors in this experiment.

Figure 2.

FT-IR spectra (A) of the GO (1), NH2-Fe3O4 (2), AMGO (3). Powder X-ray diffractogram (B) of GO (1), NH2-Fe3O4 (2) and AMGO nanocomposites (3). Room-temperature magnetisation curves (C) of AMGO nanocomposites. The inset picture in (C) showed the AMGO dispersibility and magnetic separation ability. TEM images of GO (D). SEM images of AMGO at 2.5:1 (E) and α-AMGO microreactors (F).

Materials and methods

Materials

Grapheme oxide (GO) (100602, Nanjing XFnano Materials Tech Co., Ltd. China); FeCl3·6H2O (analytically pure, Aladdin Chemistry Co., Ltd. China); α-glucosidase from Saccharomyces cerevisiae (EC 3.2.1.20, Sigma-Aldrich Chemical, St. Louis, MO); 4-nitrophenyl-α-D-glucopyranoside (pNPG) (98%, J&K Scientific Ltd. China); Dioscorea opposita Thunb. (Huai Shan Yao) was obtained from Jiaozuo city (Henan, China) in 2016 where it is famous of planting Huai Shan Yao for more than 1000 years. The voucher specimens (No. SYP20161116) of yam were stored in our lab which is in college of chemistry and chemical engineering, Henan University; batatasin I and 2,4-dimethoxy-6,7-dihydroxyphenanthrene were provided by our group,. All other solvents in this research were used as received without further purification.

Apparatus

1260 HPLC (Agilent Technologies Co., Ltd, MA); AmaZon SL ion trap mass spectrometry (Bruker Daltonik GmbH Co., Ltd, Bremen); 5C18-PAQ (4.6 (ID) × 250 (mm)) chromatography column (Cosmosil, Japan); incubator (QYC-2102C, CIMO Instrument Manufacturing Co., Ltd. Shanghai, China); microplate reader (DNM-9606, Perlong Medical Equipment Co., Ltd. Beijing, China); D8 Advance XRD characterisation (Bruker, Germany); JSM-7610F scanning electron microscope (JEOL Ltd. Japan); MPMS3 Magnetic properties (Quantum Design); VERTEX 70 infrared spectra (Bruker, Germany); JEM-2100 transmission electron microscope (JEOL Ltd. Japan).

Preparation of AMGO biocompatible nanocomposite

Amine-functionalised rGO@Fe3O4 (AMGO) nanocomposite were synthesised by a facile one-step solvothermal method using ethylene glycol (30.00 ml), anhydrous sodium acetate (0.5000 g), 1,6-hexanediamine (1.625 g) and FeCl3·6H2O (0.2500 g) as solvent, dispersing agent, nitrogen precursor and ferric source, respectively, combined with GO (0.1000 g) at 198 °C with a reaction time of 6 h. Finally, black products were washed by water and ethyl alcohol in ultrasonic condition to remove solvents and excessive 1,6-hexanediamine successfully, and then dried at 45 °C under vacuum and then stored in 4 °C for next characterisation and use. The synthesis diagrammatic illustration of biocompatible nanocomposite was shown in Figure 1(A).

Construction of α-glucosidase microreactors

The α-glucosidase was covalent interaction with the amino group of AMGO nanocomposite by employing glutaraldehyde (GA) as the crosslinking agent to construct α-glucosidase microreactors (α-AMGO). The synthesis and construction diagrammatic illustration were shown in Figure 1(A). There were some parameters, including proportion of FeCl3·6H2O to GO (w/w), glutaraldehyde concentration, immobilised time and enzyme amount, need to be optimised to obtain the optimal construction conditions of α-glucosidase microreactors. The ultimate α-glucosidase amount immobilised on AMGO nanocomposite surfaces was computed according to our previous work,. Finally, α-AMGO microreactors were stored in phosophate-buffered solution (PBS) and kept at 4 °C before use.

Application of α-AMGO microreactors for the α-glucosidase inhibitors screening

The typical screening process of this method is demonstrated in Figure 1 (B). α-AMGO microreactors (20.00 mg) and Dioscorea opposita Thunb. peel extract (solution S0) extracted as reference22 were incubated and shaken in a vortex oscillator beaker under 37 °C for 2 h. Then carefully removing of the supernatant by magnetic separation, the α-AMGO microreactors were washed several times with PBS buffer solution until washed solutions did not exist free α-glucosidase. Finally, the α-AMGO microreactors reacted with 1 ml 90% acetonitrile (v/v) for three times and the eluent solutions were carefully saved as solution S1. The rGO@Fe3O4 (AMGO) nanocomposite were employed to be negative control for the inhibitors screening to remove the nonspecific binding and the final eluent solution was named as solution S2. The inhibitor screening capacity (ISC) with α-AMGO microreactors was calculated by the following equation:

Where the ligand concentration in solution S1, S2 were referred as [C1], [C2], with [C0] being all of ligand concentration in solution S0. All of solutions S0, S1 and S2 were filtrated through 0.22 μm filter membrane when HPLC-MS system analyzed.

Results and discussion

Characterisation of AMGO nanocomposite and α-AMGO microreactors

As shown in Figure 2, the as-prepared AMGO nanocomposite were detailedly characterised by FT-IR, XRD, TEM, SEM, magnetic properties and the α-AMGO microreactors were studied by SEM. The synthesis reaction mechanism was featured by nucleophilic substitution of carboxyl groups in GO and hydroxyl in Fe3O4 in Figure 1. FT-IR spectra of the GO (1), amine-functionalised Fe3O4 (NH2-Fe3O4) (2), AMGO (3) were shown in Figure 2(A). The GO sheet showed characteristic adsorption bands with the 1735 cm−1 (carboxyl C = O), 1623 cm−1 (aromatic C = C), 1400 cm−1 (carboxyl C–O), 1219 cm−1 (epoxy C–O), 1050 cm−1 (alkoxy C–O groups) and 3409 cm−1 (hydroxy –OH group). For NH2-Fe3O4, the peaks were observed at 2917, 2848, 1488 and 890 cm−1, 585 cm−1 (Fe–O functional groups), indicating that the Fe3O4 nanoparticle were successfully functionalised with amino groups. After the solvothermal reaction of GO with 1,6-hexanediamine, the GO peak at 1400 cm−1 totally vanished, and the intensity of peak at 1735 cm−1 decreased obviously and the appearance of two new characteristic peaks (-CONH amide band I, NH amide band II) at 1640 and 1562 cm−1, respectively, in AMGO indicated the effective chemical interaction between alkyl-amines and GO by the formation of amide linkages, and the characteristic peaks (2917, 2848, 1488 and 890 cm−1) of NH2–Fe3O4 were also found in AMGO. Thus, it was concluded that AMGO was synthesised successfully from above results.

The XRD patterns of GO (1), NH2-Fe3O4 (2) and AMGO nanocomposite (3) were presented in Figure 2(B). GO showed a sharp peak centered at 2θ = 10.3° (002), indicating the presence of the oxygen functional groups,. The diffraction peaks (2θ) of the as-synthesised NH2-Fe3O4 particles at 18.2, 30.1, 35.6, 43.0, 53.5, 57.0, 62.5 were ascribed to the (111), (220), (311), (400), (422), (511), (440) of the magnetite Fe3O4, which were in accord with pure spinel Fe3O4 (JCPDS no. 89–3854),. The characteristic diffraction peaks of GO at 2θ = 10.3° had been mostly replaced by a low and broad peaks of AMGO around 2θ = 21.3°, which indicated that GO was reduced to rGO in the reaction process. The as-prepared AMGO nanocomposite presented all diffraction peaks of the NH2-Fe3O4 particles and rGO and the XRD results revealed their high crystallinity.

The magnetic properties of AMGO nanocomposite were observed at 25 °C by MPMS3 Magnetic properties system that was illustrated in Figure 2(C). The AMGO nanocomposite dispersed well in water and could be drawn to the sidewall with magnet in a few minutes and the saturation magnetisation was 0.25 emu/g from the plots of magnetisation versus field.

As shown in Figure 2(D–F), TEM of GO (D), SEM of AMGO (E) and α-AMGO microreactors (F) images were observed. GO exhibited irregular sheets with some wrinkles which provided a large surface area and many oxygen functional groups. NH2-Fe3O4 nanoparticle were strongly anchored on the surface of rGO sheets with a applicable density (shown in Figure 3(E)), implying the strong interaction between NH2-Fe3O4 particles and rGO sheets. The successful construction of α-AMGO microreactors was revealed by comparing the SEM images of AMGO nanocomposite before (E) and after conjugation (F). The morphology of AMGO nanocomposite were nearly smooth and few aggregation and the surface (F) was slightly coarse and obviously aggregated after the conjugation step, which demonstrated the successful construction of α-AMGO microreactors.

Figure 3.

Influence of different proportions of FeCl3·6H2O to GO (50:1 (A), 5:1 (B), 2.5:1 (C)), glutaraldehyde (crosslinking agent) concentration (D), immobilised time (E) and enzyme (α-glucosidase) amount (F) on the immobilised enzyme absorbance values (A405 nm).

Improvement of microreactors construction procedure Proportion of FeCl3·6H2O to GO

The synthesis of AMGO nanocomposite was studied by controlling the proportion of FeCl3·6H2O to GO (w/w) with different ratios (50:1, 5:1, 2.5:1) in Figure 3(A–C, respectively) by SEM. As shown in Figure 3(C), it exhibited clearly irregular sheets with some wrinkles of GO and homogeneous NH2-Fe3O4 nanoparticle (average particle size was 20 nm). However, if the amount of FeCl3·6H2O was increased, the surface of irregular sheets was entirely filled with nanoparticle and the wrinkles was aggregated seriously as shown in Figure 3(A,B). When the amount of FeCl3·6H2O was decreased, good morphology of AMGO could be obtained, however, their magnetic properties decreased, which would cause difficulties in subsequent magnetic separation of samples. Clearly, the aggregation was improved obviously with the increasing amount of GO. When the proportion of FeCl3·6H2O to GO (w/w) was 2.5:1, the large surface area and modest magnetic properties of AMGO nanocomposite made them appropriate to be a support for the enzyme immobilisation. Consequently, the proportion of (2.5:1) was selected for the following experiment.

Crosslinking agent concentration

Glutaraldehyde (GA) was used as crosslinking agent in this construction procedure, but it also was a denaturing reagent in high concentration, thus appropriate crosslinking agent concentration was an important optimisation parameters. Different GA concentrations (v/v, from 6 to 18%) were chosen to observe the relation between glutaraldehyde concentration and the pNP absorbance at 405 nm. As shown in Figure 3(D), the A405 values raised with different crosslinking agent concentrations increasing and then reached maximum value at 10%, then the absorbance in 405 nm remained until the concentration was 14%, but the absorbance values decreased when the concentration was between 14 and 18%. Obviously, when the crosslinking agent concentration was at low, the aldehyde process of AMGO nanocomposite was inadequate resulting in little α-glucosidase immobilised on the nanocomposite. When the glutaraldehyde concentration increased, the aldehyde process completed and the immobilised enzyme amount reached saturation status, but the active groups (–CHO) in the surface of AMGO could be multi-side combined or covered with each other in high crosslinking agent concentration leading to partial inactivation of α-glucosidase, so the A405nm absorbance decreased. Therefore, 10% glutaraldehyde was selected as the optimum crosslinking agent concentration.

Reaction time

Different immobilisation times were investigated for α-glucosidase microreactors construction with the optimum crosslinking agent concentration. From Figure 3(E), it could be found that 6 h was the optimised reaction time. When the immobilised time was less than 6 h, the immobilised procedure was incomplete and inadequate. The absorbance decreased gradually with the immobilised time increasing when the time was more than 6 h. The reason might be that the conformation changes and partial inactivation of α-glucosidase due to the vibration at a long time. Hence, the optimised reaction time was chosen as 6 h.

Enzyme amount

In order to find out the relation between the pNP absorbance (A405 nm) and the amount of enzyme, different ratios (w/w) of AMGO to α-glucosidase with proportions (200:1, 100:1, 67:1, 50:1, 33:1, 25:1, 20:1, 18:1) was studied in Figure 3(F). As was shown that the A405 nm values gradually increased with the increasing of α-glucosidase amount, and until the proportions was 25:1, the A405nm value of pNP remained unchanged. The reason could be that the immobilisation procedure was inadequate in low proportion of AMGO to enzymes (w/w) resulting in less pNPG was hydrolyzed, and when the ratio was more than 25:1, it could be found that the absorbance value (A405 nm) changed unobviously due to its saturation. Thus, the optimised α-glucosidase amount was the proportion of 25:1. The immobilised amount of α-glucosidase on enzyme microreactors surface was about 40 μg α-glucosidase/mg nanocomposite that was about five times of the amount in our previous work. The large immobilisation enzyme amount could obviously improve the application of α-glucosidase microreactors for inhibitors screening from natural products.

Identification of α-glucosidase inhibitors with α-glucosidase microreactors

α-AMGO microreactors were employed to screen the ligand from Chinese Yam peel extract and different proportions of denaturing solvent were also observed to separate the ligand that specifically bound to the α-AMGO microreactors in this work. As shown in Figure 4, different proportions of denature solvent (acetonitrile-water (V/V), 10, 30, 50, 70, 90, 100%) were studied by comparing the peak signal of specifically bound inhibitors to screen the optimised denaturing solvent. From the peak signals trend of two inhibitors in Figure 4, the 90% (V/V) acetonitrile-water had the optimum effect of the solubility and the separation efficiency of the screening ligand. Figure 5 showed the typical HPLC chromatograms of the solutions S0 (a), the eluent solutions S1 (the first eluent (b), the second eluent (c) and the third eluent (d)) as well with the blank eluent S2 (e) and the necessary MS1/MS informations (1, 2, 3, 4) of the specifically bound inhibitors to α-AMGO microreactors. The HPLC-MS conditions were as reference22: chromatographic column: COSMOSIL 5C18-PAQ (4.6 ID ×250 mm, 5 μm); A HPLC gradient program: solvent A (methanol) and solvent B (water), 60% A at 0–6 min, 60–50% A at 6–8 min, 50–70% A at 8–15 min and 70% A at 15–30 min, flow rate: 1 ml min−1, column temperature: 30 °C, injection volume: 5 μL, and detected wavelength: 274 nm; the capillary voltage was 4.5 kV; the endplate offset voltage was 500 V; the nebulizer pressure source was set at 15 psi; the flow rate of dry gas was 8 L min−1; and the dry temperature and vaporiser temperature were 220 °C and 300 °C. Comparing with the Dioscorea opposita Thunb. peel extract and blank solution, two ligands named as 2,4-trimethoxy-6,7-dihydroxyphe-nanthrene, Batatasin I (structures were shown in the top of Figure 5), were found to be specifically bound to the α-AMGO microreactors. It could be seen that, after three times' elution, the bound two inhibitors were almost completely washed off from the α-AMGO microreactors, which suggested that the components strongly and stably bound with α-glucosidase microreactors. Figure 5 (1, 2, 3, 4) displayed the useful mass spectral characteristics of two ligand. The immobilised amount and binding capacities of α-glucosidase were shown in Table 1. when the α-AMGO microreactors was employed as baits for inhibitors fishing, the immobilised amount and binding capacities were about more than five times, one and a half, and four times higher, respectively. The reason might be that the composition of GO with Fe3O4 not only increased the large active groups for the enzyme immobilisation, but also maintained excellent magnetic separation ability. And two specifical bound ligand were latent candidates for the antidiabetic agents development by the observing the inhibition assay,. Thus, the construction idea of α-glucosidase microreactors based on AMGO was an effective, feasible and sensitive method for the specifical separation of biological active compounds from the complex nature products.

Figure 4.

Effect of different proportions of denature solvent (acetonitrile-water (V/V), 10%, 30%, 50%, 70%, 90%, 100%).

Figure 5.

Typical HPLC chromatograms of solutions S0 (a), the eluent solutions S1 (the first eluent (b), the second eluent (c) and the third eluent (d)) and the blank eluent S2 (e) and the necessary MS1/MS informations (1, 2, 3, 4) of the specifically bound inhibitors to α-AMGO microreactors. The inset were the chemical structurals of trapped ligands. Conditions were as reference 22.

Table 1.

The immobilisation amount and binding capacities of α-glucosidase by the enzyme microreactors construction to screen potential ligands from complex natural resources.

	This work	Reference17	Reference22
immobilisation amount (μg/mg)	40	—	8.04
binding capacities (%)	35.6%/68.2%	14.04%/12.77%	26.7%/15.6%

Conclusions

In short, a typical one-pot solvothermal method was exploited to prepare amine-functionalised magnetite nanocomposite containing rGO and Fe3O4 and the nanocomposite were selected as support matrix for the covalent immobilisation of α-glucosidase to successfully construct the enzymatic microreactors. The data presented in this paper provided strong evidence that the rGO@Fe3O4 nanocomposite exhibited significantly higher immobilisation efficiency and binding capacities that could be employed as supports for the enzyme microreactors construction to screen the potential ligand from natural product resources.