Juan Liu1, Li Cui2, Xinmin Yan1, Xinyuan Zhao3, Jinluo Cheng1, Lei Zhou1, Jianbo Gao1, Zhong Cao2, Xinhua Ye1, Shen Hu2. 1. Changzhou Second People's Hospital, Nanjing Medical University, Changzhou, China. 2. School of Dentistry, University of California, Los Angeles, California, USA. 3. Department of Endodontics, Stomatological Hospital, Southern Medical University, Guangzhou, China.
Abstract
BACKGROUND/AIMS: Microbes reside in a number of body sites, including the oral cavity, and are associated with the progression of many systemic diseases. In this study, we aimed to investigate the effects of gout and hyperuricemia (HUA) on the composition of oral microbiomes. METHODS: Analysis of the oral microbiota from 12 gout patients, 11 HUA patients, and 19 healthy control subjects was performed using a deep sequencing approach, and validation of significant changes in Prevotella intermedia and Serratia marcescens in new patient cohorts was performed using quantitative PCR (qPCR). RESULTS: Our analysis indicated that both gout and HUA significantly altered the composition of the oral microbiome in patients. Patients with gout or HUA had significantly greater levels of salivary Prevotella intermedia but significantly lower levels of Serratia marcescens than healthy control subjects. CONCLUSION: We demonstrated the association between the oral microbiome and gout and HUA for the first time. In particular, 16S sequencing and qPCR analysis revealed significantly higher levels of oral Prevotella intermedia in gout/HUA patients, which suggests that these patients might be at risk for the development of periodontitis.
BACKGROUND/AIMS: Microbes reside in a number of body sites, including the oral cavity, and are associated with the progression of many systemic diseases. In this study, we aimed to investigate the effects of gout and hyperuricemia (HUA) on the composition of oral microbiomes. METHODS: Analysis of the oral microbiota from 12 goutpatients, 11 HUA patients, and 19 healthy control subjects was performed using a deep sequencing approach, and validation of significant changes in Prevotella intermedia and Serratia marcescens in new patient cohorts was performed using quantitative PCR (qPCR). RESULTS: Our analysis indicated that both gout and HUA significantly altered the composition of the oral microbiome in patients. Patients with gout or HUA had significantly greater levels of salivary Prevotella intermedia but significantly lower levels of Serratia marcescens than healthy control subjects. CONCLUSION: We demonstrated the association between the oral microbiome and gout and HUA for the first time. In particular, 16S sequencing and qPCR analysis revealed significantly higher levels of oral Prevotella intermedia in gout/HUA patients, which suggests that these patients might be at risk for the development of periodontitis.
Authors: Hannah Fehlner-Peach; Cara Magnabosco; Varsha Raghavan; Jose U Scher; Adrian Tett; Laura M Cox; Claire Gottsegen; Aaron Watters; John D Wiltshire-Gordon; Nicola Segata; Richard Bonneau; Dan R Littman Journal: Cell Host Microbe Date: 2019-11-13 Impact factor: 21.023