Chelsey L Dunham1, Aaron M Chamberlain2, Gretchen A Meyer3, Spencer P Lake4. 1. Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri, USA. 2. Department of Orthopaedic Surgery, Washington University in St Louis, St Louis, Missouri, USA. 3. Program in Physical Therapy, Washington University in St Louis, St Louis, Missouri, USA. 4. Department of Mechanical Engineering and Materials Science, Washington University in St Louis, 1 Brookings Drive, Campus Box 1185, St. Louis, Missouri, 63130, USA.
Abstract
INTRODUCTION: Posttraumatic elbow contracture is clinically challenging because injury often disrupts multiple periarticular soft tissues. Tissue specific contribution to contracture, particularly muscle, remains poorly understood. METHODS: In this study we used a previously developed animal model of elbow contracture. After surgically inducing a unilateral soft tissue injury, injured limbs were immobilized for 3, 7, 21, and 42 days (IM) or for 42 IM with 42 days of free mobilization (42/42 IM-FM). Biceps brachii active/passive mechanics and morphology were evaluated at 42 IM and 42/42 IM-FM, whereas biceps brachii and brachialis gene expression was evaluated at all time points. RESULTS: Injured limb muscle exhibited significantly altered active/passive mechanics and decreased fiber area at 42 IM but returned to control levels by 42/42 IM-FM. Gene expression suggested muscle growth rather than a fibrotic response at 42/42 IM-FM. DISCUSSION: Muscle is a transient contributor to motion loss in our rat model of posttraumatic elbow contracture. Muscle Nerve 58:843-851, 2018.
INTRODUCTION:Posttraumatic elbow contracture is clinically challenging because injury often disrupts multiple periarticular soft tissues. Tissue specific contribution to contracture, particularly muscle, remains poorly understood. METHODS: In this study we used a previously developed animal model of elbow contracture. After surgically inducing a unilateral soft tissue injury, injured limbs were immobilized for 3, 7, 21, and 42 days (IM) or for 42 IM with 42 days of free mobilization (42/42 IM-FM). Biceps brachii active/passive mechanics and morphology were evaluated at 42 IM and 42/42 IM-FM, whereas biceps brachii and brachialis gene expression was evaluated at all time points. RESULTS: Injured limb muscle exhibited significantly altered active/passive mechanics and decreased fiber area at 42 IM but returned to control levels by 42/42 IM-FM. Gene expression suggested muscle growth rather than a fibrotic response at 42/42 IM-FM. DISCUSSION: Muscle is a transient contributor to motion loss in our rat model of posttraumatic elbow contracture. Muscle Nerve 58:843-851, 2018.
Authors: Chelsey L Dunham; Ryan M Castile; Necat Havlioglu; Aaron M Chamberlain; Leesa M Galatz; Spencer P Lake Journal: J Shoulder Elbow Surg Date: 2017-01-10 Impact factor: 3.019
Authors: Alex J Reiter; Hayden R Schott; Ryan M Castile; Paul C Cannon; Necat Havlioglu; Aaron M Chamberlain; Spencer P Lake Journal: J Orthop Res Date: 2020-12-02 Impact factor: 3.102
Authors: Michael A David; Alex J Reiter; Chelsey L Dunham; Ryan M Castile; James A Abraham; Leanne E Iannucci; Ishani D Shah; Necat Havlioglu; Aaron M Chamberlain; Spencer P Lake Journal: Front Bioeng Biotechnol Date: 2022-02-21