G Chen1, H Zhou. 1. Department of Orthopedic Surgery, Second Affiliated Hospital of Dalian Medical University, Dalian, China. zhouhuan8103@126.com.
Abstract
OBJECTIVE: The functions of miRNA-708 for various diseases have been confirmed. However, its roles in osteosarcoma are unclear. In this study, we aimed to explore the role of miRNA-708 in osteosarcoma. PATIENTS AND METHODS: Detection of the expression of miRNA-708 and CUL4B was used by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Cells were transfected with miRNA-708 mimics (mimics group) and miRNA negative control (NC group). Detection of cell growth curve at 24 h, 48 h, 72 h, and 96 h was made by cell counting kit-8 (CCK-8). Examination of the apoptosis rate was made by flow cytometry. The identification of the regulatory function was made by the luciferase reporter assay. The expression level of CUL4B was detected by Western blot. RESULTS: MiRNA-708 expression was reduced in the tumor cell lines. Compared with NC group, miRNA-708 expression was up-regulated by transfecting with mimics. Lower proliferation efficiency and higher cell apoptosis were showed in miRNA-708 mimics group relative to NC group. MiRNA-708 could regulate the expression of CUL4B by binding to its 3'UTR area. Furthermore, lower miRNA-708 and higher CUL4B were expressed in tumor tissues. MiRNA-708 expression was lower in tissues with IIB-III stage than that in IA-IIA stage. CONCLUSIONS: MiRNA-708/CUL4B axis contributes into cell proliferation and apoptosis of osteosarcoma.
OBJECTIVE: The functions of miRNA-708 for various diseases have been confirmed. However, its roles in osteosarcoma are unclear. In this study, we aimed to explore the role of miRNA-708 in osteosarcoma. PATIENTS AND METHODS: Detection of the expression of miRNA-708 and CUL4B was used by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Cells were transfected with miRNA-708 mimics (mimics group) and miRNA negative control (NC group). Detection of cell growth curve at 24 h, 48 h, 72 h, and 96 h was made by cell counting kit-8 (CCK-8). Examination of the apoptosis rate was made by flow cytometry. The identification of the regulatory function was made by the luciferase reporter assay. The expression level of CUL4B was detected by Western blot. RESULTS:MiRNA-708 expression was reduced in the tumor cell lines. Compared with NC group, miRNA-708 expression was up-regulated by transfecting with mimics. Lower proliferation efficiency and higher cell apoptosis were showed in miRNA-708 mimics group relative to NC group. MiRNA-708 could regulate the expression of CUL4B by binding to its 3'UTR area. Furthermore, lower miRNA-708 and higher CUL4B were expressed in tumor tissues. MiRNA-708 expression was lower in tissues with IIB-III stage than that in IA-IIA stage. CONCLUSIONS:MiRNA-708/CUL4B axis contributes into cell proliferation and apoptosis of osteosarcoma.