Literature DB >> 3022935

Evidence for trans splicing in trypanosomes.

R E Sutton, J C Boothroyd.   

Abstract

The 5' ends of trypanosome mRNAs consist of an identical sequence of 35 nucleotides. This "mini-exon" sequence is derived from the 5' end of a 137 nucleotide RNA (medRNA). The remainder of each mRNA is derived from a protein-coding exon that is not linked to the mini-exon. We propose that medRNA is spliced in trans to de-novo-initiated transcripts of protein-coding genes. This trans splicing model predicts that the downstream portion of medRNA will be part of a branched structure and then be released as a free product (minRNA). We demonstrate that significant levels of minRNA exist in trypanosome RNA. Furthermore, minRNA can be released from high molecular weight RNA by a HeLa cell S100 "debranching" extract. We conclude that trans splicing is the physiological process by which mature mRNA molecules are synthesized in trypanosomes.

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Year:  1986        PMID： 3022935      PMCID： PMC7133315          DOI： 10.1016/0092-8674(86)90617-3

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  45 in total

1.  An RNA processing activity that debranches RNA lariats.

Authors:  B Ruskin; M R Green
Journal:  Science       Date:  1985-07-12       Impact factor: 47.728

2.  A 5' spliced leader is added in trans to both alpha- and beta-tubulin transcripts in Trypanosoma brucei.

Authors:  S Sather; N Agabian
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

3.  RNA splicing is required to make the messenger RNA for a variant surface antigen in trypanosomes.

Authors:  L H Van der Ploeg; A Y Liu; P A Michels; T De Lange; P Borst; H K Majumder; H Weber; G H Veeneman; J Van Boom
Journal:  Nucleic Acids Res       Date:  1982-06-25       Impact factor: 16.971

4.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

5.  Leader sequences of murine coronavirus mRNAs can be freely reassorted: evidence for the role of free leader RNA in transcription.

Authors:  S Makino; S A Stohlman; M M Lai
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

6.  Sequences homologous to variant antigen mRNA spliced leader in Trypanosomatidae which do not undergo antigenic variation.

Authors:  R G Nelson; M Parsons; M Selkirk; G Newport; P J Barr; N Agabian
Journal:  Nature       Date:  1984 Apr 12-18       Impact factor: 49.962

7.  Structure and expression of a Trypanosoma brucei gambiense variant specific antigen gene.

Authors:  E Pays; M Lheureux; M Steinert
Journal:  Nucleic Acids Res       Date:  1982-05-25       Impact factor: 16.971

8.  Chromosome rearrangements in Trypanosoma brucei.

Authors:  L H Van der Ploeg; A W Cornelissen; P A Michels; P Borst
Journal:  Cell       Date:  1984-11       Impact factor: 41.582

9.  Structure and regulated expression of genes encoding fructose biphosphate aldolase in Trypanosoma brucei.

Authors:  C E Clayton
Journal:  EMBO J       Date:  1985-11       Impact factor: 11.598

10.  Discontinuous synthesis of mRNA in trypanosomes.

Authors:  J M Kooter; T De Lange; P Borst
Journal:  EMBO J       Date:  1984-10       Impact factor: 11.598
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  204 in total

1.  A new twist in trypanosome RNA metabolism: cis-splicing of pre-mRNA.

Authors:  G Mair; H Shi; H Li; A Djikeng; H O Aviles; J R Bishop; F H Falcone; C Gavrilescu; J L Montgomery; M I Santori; L S Stern; Z Wang; E Ullu; C Tschudi
Journal:  RNA       Date:  2000-02       Impact factor: 4.942

2.  Molecular cloning and expression of adenosine kinase from Leishmania donovani: identification of unconventional P-loop motif.

Authors:  K M Sinha; M Ghosh; I Das; A K Datta
Journal:  Biochem J       Date:  1999-05-01       Impact factor: 3.857

3.  trans splicing of polycistronic Caenorhabditis elegans pre-mRNAs: analysis of the SL2 RNA.

Authors:  D Evans; T Blumenthal
Journal:  Mol Cell Biol       Date:  2000-09       Impact factor: 4.272

4.  Operons and SL2 trans-splicing exist in nematodes outside the genus Caenorhabditis.

Authors:  D Evans; D Zorio; M MacMorris; C E Winter; K Lea; T Blumenthal
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-02       Impact factor: 11.205

5.  Genome annotation by high-throughput 5' RNA end determination.

Authors:  Byung Joon Hwang; Hans-Michael Müller; Paul W Sternberg
Journal:  Proc Natl Acad Sci U S A       Date:  2004-02-02       Impact factor: 11.205

Review 6.  trans and cis splicing in trypanosomatids: mechanism, factors, and regulation.

Authors:  Xue-hai Liang; Asaf Haritan; Shai Uliel; Shulamit Michaeli
Journal:  Eukaryot Cell       Date:  2003-10

7.  Presence of a poly(A) binding protein and two proteins with cell cycle-dependent phosphorylation in Crithidia fasciculata mRNA cycling sequence binding protein II.

Authors:  Bidyottam Mittra; Dan S Ray
Journal:  Eukaryot Cell       Date:  2004-10

8.  Interaction between retroviral U5 RNA and the T psi C loop of the tRNA(Trp) primer is required for efficient initiation of reverse transcription.

Authors:  A Aiyar; D Cobrinik; Z Ge; H J Kung; J Leis
Journal:  J Virol       Date:  1992-04       Impact factor: 5.103

9.  Characterization of the cDNA and genomic sequence of a G protein gamma subunit (gamma 5).

Authors:  K J Fisher; N N Aronson
Journal:  Mol Cell Biol       Date:  1992-04       Impact factor: 4.272

10.  Novel and essential subunits in the 300-kilodalton nuclear cap binding complex of Trypanosoma brucei.

Authors:  Hongjie Li; Christian Tschudi
Journal:  Mol Cell Biol       Date:  2005-03       Impact factor: 4.272
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