Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose.

The dataset presented in this article is related to the research article entitled "One-pot, two-step transaminase and transketolase synthesis of l-gluco-heptulose from l-arabinose" (Bawn et al., 2018 in press) [1]. This article presents data on initial experiments that were carried out to investigate new thermostable transketolase (TK) activities with l-arabinose. Transaminase (TAm) sequences from an in-house library of thermophilic strains were analyzed to compare homologies to characterized TAms with desired activity. DNA and amino acid sequences are presented for all the enzymes investigated. Calibration curves for products of the TK and TAm reactions are also presented along with chromatographic analysis of the various one-pot reactions.

Specifications Table

Value of the data

The data presented in this article gives new insight into the activities of thermostable enzymes not published before.

The data represents a rationale behind why TKs and TAms were selected for the one-pot reaction.

Product of one-pot reaction, l-gluco-heptulose, is a pharmaceutically-relevant compound.

Data

l-Arabinose is a major monosaccharide of sugar beet pulp (SBP), a by-product of sucrose extraction which is currently produced and sold as a low value animal feed [1]. The main focus of this work was to create a value-added product from the monosaccharides that make up SBP via enzymatic routes. Building on previous work [2], [3], this present study produces l-gluco-heptulose, a high value, pharmaceutically relevant compound from l-arabinose using a two-step thermostable enzyme cascade. A thermostable TK catalyzed the synthesis of l-gluco-heptulose from l-arabinose and β-hydroxypyruvate (HPA) in which the latter was produced in situ from l-serine and α-ketoglutaric acid using a thermostable TAm.

Table 1 identifies thermostable TKs utilized and whether they were active towards l-arabinose via the Seliwanoff assay [4]. Table 2 describes the TAms investigated and compares sequence homologies to TAms previously showing activities required for this reaction. Examples of HPAEC-PAD traces (Fig. 2, Fig. 3) demonstrate how the TK and TAm one-pot reactions were monitored for the presence of l-gluco-heptulose.

Table 1

TKs showing activity with l-arabinose via Seliwanoff assay.

TK Strain	UniProtKB accession code	Plasmid name and abbreviated name	Active towards l-arabinose from Seliwanoff assay
Deinococcus geothermalis DSM 11300	Q1IW07	pQR1758 (TKDgeo)	✓
Deinococcus radiodurans DSM 20539	Q9RS71	pQR1759 (TKDrad)	✓
Geobacillus stearothermophilus DSM 22	KFL15812.1	pQR1743	✓
Thermobifida fusca strain YX	Q47ND4	pQR1744	✗
Thermotoga maritima DSM 3109	Q9X283	pQR1745	✗

Table 2

Sequence similarity values between new cloned TAms and DGEO_0713, SPAT and CV2025.

TAm Strain	UniProtKB accession code	Plasmid name and abbreviated name	Homology to DGEO_0713	Homology to SPAT	Homology to CV2025
			Deinococcus geothermalis(%)	Sulfolobus solfataricus(%)	Chromobacterium violaceum(%)
Deinococcus radiodurans DSM 20539	Q9RWP3	pQR1746	78	38	31
Geobacillus stearothermophilus DSM 22	Q59228	pQR1756 (TAmGste)	25	38	40
Thermobifida fusca strain YX	Q47LH8	pQR1748	26	37	41
Thermotoga maritima DSM 3109	G4FE93	pQR1749	30	58	31
Deinococcus geothermalis DSM 11300	Q1IZC2	pQR1757 (TAmDgeo)	29	29	44
Xanthomonas campestris pv. Campestris DSM 3586	Q8PDQ2	pQR1751	28	25	30
Thermotoga maritima DSM 3109	Q9X1C0	pQR1752	33	34	36
Pectobacterium carotovorum subsp. Carotovorum DSM 30168	A0A0B3YSH6	pQR1755	24	31	33

Fig. 2

HPAEC-PAD trace showing l-gluco-heptulose production from one-pot reaction with TAmDgeo and TKDgeo after 24 h.

Fig. 3

HPAEC-PAD trace showing l-gluco-heptulose production from one-pot reaction with TAmDgeo and TKDrad after 24 h.

Experimental design, materials and methods

TK activity

Thermostable TKs were cloned and subsequently expressed in E.coli BL21 DE3. Cell lysates were used to determine activity towards l-arabinose using the colorimetric assay, Seliwanoff assay. The Seliwanoff assay distinguishes between ketoses and aldoses using 6 M HCl and resorcinol (Seliwanoff׳s reagent) [4]. After 24 h incubation of enzyme and l-arabinose, Seliwanoff reagent was added to the reaction and heated at 100 °C. Colour formation due to the presence of the ketose, l-gluco-heptulose, was observed within 15 min (Table 1).

TAm sequence analysis

TAm sequences were obtained from the NCBI database [5] and the UniProt Knowledgebase (UniProtKB) [6] followed by a sequence alignment using Clustal W [7].

Product analysis using HPAEC-PAD

Quantitative analysis of l-arabinose, l-gluco-heptulose and HPA was performed using HPAEC-PAD (ICS 5000+, Dionex) equipped with a Dionex AminopacTM PA1 anion exchange column 4×250 mm2 fitted with a Dionex AminopacTM PA1 guard column 4×50 mm2, an electrochemical detector system, and an eluent generator with a KOH 500 cartridge. The elution times of each compound can be observed in Fig. 1. Fig. 3, Fig. 4 are examples of a one-pot reaction analysis with various TKs and TAmDgeo. Standard calibration curves of l-gluco-heptulose and HPA were used for quantification purposes (Fig. 4, Fig. 5).

Fig. 1

HPAEC-PAD trace showing the elution of l-arabinose, l-gluco-heptulose and HPA.

Fig. 4

Calibration curve for determination of l-gluco-heptulose yield.

Fig. 5

Calibration curve for determination of HPA yield.

TK DNA/ amino acid sequences

DNA sequences were retrieved from the NCBI database [5] and amino acid sequences were obtained through the UniProtKB [6].

>TKDgeo

ATGAGTCCCGAACAGCAGGCCGTGCGTCAGGATGTCGATCAGCTGAGCATCAACACCATCAGGACGCTTGCCATCGATGCGGTGCAGCGGGCCAACAGTGGCCACCCCGGCGCGCCGCTCGGCATGGCCCCGATGGGCTACGTGCTGTGGCAGCGCTTCCTGCGCCACAATCCGAAACATCCCGAGTGGCCGGGCCGCGACCGCTTCGTGCTGTCGGCAGGGCACGCCAGCATGCTGATCTACTCGCTGCTGCACCTCACCGGCTACGACCTGCCGCTGGAGGACATCAAGAACTTCCGCCAGTGGGGCAGCAAGACGCCTGGGCATCCCGAGTTCTTCCACACCCCAGGCCTAGACGCCACCACCGGCCCGCTCGGTCAGGGTGCGGCGATGACGGTGGGCATGGCGATGGCCGAAGCGCACCTCGCCGCACGCTACAACCGCCCCGGCTTCAAGGTCTTTGACAACTACACCTACGCGATCTTGGGGGACGGCGACCTGCAAGAAGGCGTCAACCACGAGGCCGCGTCGCTGGCAGGGCACCTCAAGCTGGGCAAGCTGATCTGGCTGCACGACGACAACCAGGTGCAGCTGGACACCGCCACGTTCAAGGCGGCCAACGAGGATACTGCGGAGCGTTACCGCGCCTACGGCTGGGAAGTTCTGCGTGTGCAAGACGGCAACAATCTCACGGAGATCGAGAACGCGATCCGCCAGGCACGGATGAACACCGAGCAGCCCACCCTGATCCAGGTTCGCACGGTGATCGGCTTCGGCAGTCCCCGTGCGGGCACCAGCAAGGCGCACGGCGAGCCGCTGGGCGAGGAAGGCGTGCAGGAGACCAAGGCGGCCCTGGGCTGGGACTACCCGCCCTTCACGGTGCCCGACGAGGTCAAGGCGCATATGGACGCGACTGAGCGTGGCGCGGAGTGGGAGGCCGACTGGAACGCGCTGATGGAGCGCTACCGTGCCGAGTACCCCGATCTCGCGGCGGAGGTTGACGCGCTGCTGGCGCGCGAACTGCCCGCCAATCTCGCCGAAGTGCTCCCCTCCTACGAAGTGGGCAGCAAGGCCATCGCCACCCGCAACGCGAGCGGTGAAGTCATCAATGCGCTGGCGCAGGTGGTGCCGGGCCTGATGGGGGGCAGTGCGGATCTCTCCGGCAGCACCAAAACCACCATCAAGGACGGCGGCGAGTTTCTGCCAGGAAACTACGGGGGCCGCAACGTCTACTTTGGCGTCCGCGAGTTTGGGATGGCCGCAGCGGGCAATGGCCTTTCGCTCTACGGAGGTGTTCGGCCCCTGGTGGGGACCTTCCTGGTGTTTGCGGACTACCTCAAGCCCGCCTTCCGCCTCTCCGCCCTTCAGTTCCAGCCGGTTACCTATGTCCTGACCCATGACTCCATTGGCCTGGGCGAAGACGGCCCAACCCACCAGCCTATTGAGCAGCTCGCCATGCTGCGCGCCGTGCCGGGTGCCCACGTGATTCGCCCCGCCGACGCCAACGAGACGGCGGCGGCCTGGCAGATGGCGCTGGAGTACGACAAGGGACCAACCGCTCTGGCCCTCTCCCGCCAGGATCTCCCAGTGCTGCCCCGCAACCACGCGGGCGTGAAGAAGGGCGCCTACGTGGTTCGCGACGCCGAAGGGGGGCCGGCACAGATCATCTTGATCGCCACCGGCTCGGAGGTGAGCCTGGCGCTGGATGCTGCCCAAGCGCTGGCGGAGGAAGGCATCCAGGCTCGGGTCGTCTCAATGCCCTGCATGGAAGTCTTCCGCCAGCAGGACGCCAGTTATCGGGACAGCGTGCTCACCCCCGGCGTGAAACGCGTGGCCATCGAGGCTGCCAGCCCGCTCCCCTGGTATGAGTGGGTGGGCTTTGACGGCGCGGTGATCGGAATGACCACCTTTGGCGCCTCGGCCCCAGCCAAAGTCCTCTTTGAGAAATTCGGCTTCAACGTGCCGAACGTCGTGCAGGTCGTCAAGGGCGTTTTGCAGAGGTGA

>TKDgeo

MSPEQQAVRQDVDQLSINTIRTLAIDAVQRANSGHPGAPLGMAPMGYVLWQRFLRHNPKHPEWPGRDRFVLSAGHASMLIYSLLHLTGYDLPLEDIKNFRQWGSKTPGHPEFFHTPGLDATTGPLGQGAAMTVGMAMAEAHLAARYNRPGFKVFDNYTYAILGDGDLQEGVNHEAASLAGHLKLGKLIWLHDDNQVQLDTATFKAANEDTAERYRAYGWEVLRVQDGNNLTEIENAIRQARMNTEQPTLIQVRTVIGFGSPRAGTSKAHGEPLGEEGVQETKAALGWDYPPFTVPDEVKAHMDATERGAEWEADWNALMERYRAEYPDLAAEVDALLARELPANLAEVLPSYEVGSKAIATRNASGEVINALAQVVPGLMGGSADLSGSTKTTIKDGGEFLPGNYGGRNVYFGVREFGMAAAGNGLSLYGGVRPLVGTFLVFADYLKPAFRLSALQFQPVTYVLTHDSIGLGEDGPTHQPIEQLAMLRAVPGAHVIRPADANETAAAWQMALEYDKGPTALALSRQDLPVLPRNHAGVKKGAYVVRDAEGGPAQIILIATGSEVSLALDAAQALAEEGIQARVVSMPCMEVFRQQDASYRDSVLTPGVKRVAIEAASPLPWYEWVGFDGAVIGMTTFGASAPAKVLFEKFGFNVPNVVQVVKGVLQR

>TKDrad

ATGACAGACCAGAGCGTTTCCCAAAACGTGGCGCGGCTGAGTGTGAACACCATTCGCACGCTCGCCATTGACGCGGTGCAGGCCGCCAACTCGGGCCACCCCGGTGCGCCGCTGGGCATGGCCCCGATGGGCTACGTGCTGTGGCACAAGTTCCTGCGCCACAACCCCGCGCACCCTGAGTGGCCGGGCCGCGACCGCTTCGTGCTGTCGGCGGGGCACGCCTCCATGCTGATCTACAGCCTGCTGCACCTGACCGGCTACCAGGAAATGACGCTCGACGACCTGCGCCACTTCCGGCAGTGGGGCTACCACACCCCCGGCCACCCCGAGTTTTTCCACACCAAGGGTCTGGACGCGACCACCGGCCCGCTTGGGCAGGGCGCGGCGATGACGGTGGGCATGGCGATGGCCGAAGCACACCTCGCCGCCCGCTACAACCGCGAAGGCTTTCCGATTTTCGACAACCGCACCTACGCCATCATGGGCGACGGCGATCTGCAAGAAGGCATCAACCACGAAGCCGCCGCGCTCGCCGGGCACCTGAAACTCGGCAAGCTGATCTGGCTGCACGACGACAACCACATCCAGCTCGACACGCCCACGAACAAGGCCGAGTCCGAGGACACCGCCGCCCGCTTCCGCGCCTATGGCTGGAACGTGCTGAAGGTGGAAGACGGCGACAATCTGGACGAAATTGAAAAGGCGATTGCCGAGGCCCGCAGCCAAAGCGAGCGGCCCACGCTGATTCAGGTGCGCACCATCATCGGCTTCGGCAGCCCGCGCGCCGGCACGAGCAAGGCGCACGGCGAGCCGCTCGGCGAAGAGGGCGTGGCCGAGACGAAGAAAGCGTTGGGCTGGGAGTACCCCGCTTTTACCGTGCCCGACGAAGTGGCTGCGCACATGAACGCTCGCGCTAAGGGTGCTCAACTCGAAGCCGACTGGGAAAAACTGATGGCCGACTACCGCACCGCGCACCCCGACCTCGGCAAGGAAGTGGACGCGCTGCTCGCCCGTGAACTGCCCGCCAACCTCGCCGACCTGCTGCCCAAGTACGAAGTCGGCGGCAAGGCGGCGGCCACCCGCAACGCGAGCGGCGAAGTCATCAACGCGCTGGCGAAGGTGCTTCCCGGTTTGATGGGCGGCAGCGCGGACCTCTCGGGCTCGACCAAGACCACCATCAAGGACGGCGGCGAGATGGAAGCGGGCACCATGGGCGGGCGCAACGTGCTGTTCGGCGTGCGCGAGTTCGGCATGAGCGCCGCGGGCAATGGCCTGAGCCTCTACGGCGGCCTGCACCCGATGGTAGGCACCTTCCTGGTATTCGCCGACTACCTCAAGCCGGCTTTCCGCCTCTCGGCGCTGCAAATGCAGCCGGTGACTTACGTGCTGACCCACGACTCCATCGGTCTGGGCGAAGACGGGCCGACCCACCAGCCGGTGGACCAGCTCGCCATGCTGCGAGCGGTGCCGGGCGCCCACGTCATTCGCCCCGCCGACGCCAACGAAACCGCCGCCGCGTGGCTGATGGCGCTGGAATACGACAAGGGCCCCACCGCGCTCGCCCTCTCGCGCCAGGATCTGCCGATTCTGCCCGCCAACATCGAAGGCGTGAAGAAGGGCGCGTATGTCCTCCGAGATGTGGACGGTGCCGATGGTCAGGGGGCTCAAGTCATCCTGATC

GCCAGCGGCTCGGAAGTCGCCCTGGCCCTGAGCAGCGCCGAGCGGCTGGCCGAAGAGGGCGTGCAGGCCCGCGTGGTGTCCATGCCGTGCATGGAGGTCTTTCGCCAGCAGGAGCAGAGCTACCGCGACAGCGTGCTGACCCCCGGCGTGAAGCGCGTCGCCATCGAGGCCGCCAGCCCGCAGCCCTGGTACGAGTGGACGCTCGGCGGCCCAGTCATCGGCATGACGACCTTCGGTGCGTCGGCCCCGGCCAAGGTGCTGTTTGAGAAGTTCGGCTTCAGCGTGGAAAACGTGGTGAAGGTGGTCCACTCCGTGCTGTAA

>TKDrad

MTDQSVSQNVARLSVNTIRTLAIDAVQAANSGHPGAPLGMAPMGYVLWHKFLRHNPAHPEWPGRDRFVLSAGHASMLIYSLLHLTGYQEMTLDDLRHFRQWGYHTPGHPEFFHTKGLDATTGPLGQGAAMTVGMAMAEAHLAARYNREGFPIFDNRTYAIMGDGDLQEGINHEAAALAGHLKLGKLIWLHDDNHIQLDTPTNKAESEDTAARFRAYGWNVLKVEDGDNLDEIEKAIAEARSQSERPTLIQVRTIIGFGSPRAGTSKAHGEPLGEEGVAETKKALGWEYPAFTVPDEVAAHMNARAKGAQLEADWEKLMADYRTAHPDLGKEVDALLARELPANLADLLPKYEVGGKAAATRNASGEVINALAKVLPGLMGGSADLSGSTKTTIKDGGEMEAGTMGGRNVLFGVREFGMSAAGNGLSLYGGLHPMVGTFLVFADYLKPAFRLSALQMQPVTYVLTHDSIGLGEDGPTHQPVDQLAMLRAVPGAHVIRPADANETAAAWLMALEYDKGPTALALSRQDLPILPANIEGVKKGAYVLRDVDGADGQGAQVILIASGSEVALALSSAERLAEEGVQARVVSMPCMEVFRQQEQSYRDSVLTPGVKRVAIEAASPQPWYEWTLGGPVIGMTTFGASAPAKVLFEKFGFSVENVVKVVHSVL

>pQR1743

ATGGCGCATTCGATCGAAGAATTAGCGATTACCACCATTCGAACGCTGTCGATTGACGCGATCGAAAAAGCGAAATCCGGGCACCCGGGCATGCCGATGGGCGCGGCCCCGATGGCGTATACGCTCTGGACGAAATTTATGAACCATAATCCAGCGAATCCCAACTGGTTTAACCGCGACCGGTTTGTTTTGTCCGCTGGGCACGGGTCGATGCTGCTTTACAGCCTGCTTCATCTAAGCGGCTACGATGTCACGATGGACGACTTGAAACAGTTCCGCCAATGGGGAAGCAAAACGCCGGGCCATCCGGAATACGGCCATACGCCAGGGGTGGAGGCAACGACCGGCCCGCTCGGCCAAGGGATTGCGATGGCGGTCGGCATGGCGATGGCGGAACGGCATTTGGCGGCTGCATACAATCGCGATGGATTTGACATTATCAACCACTACACGTATGCGATTTGCGGCGACGGCGATTTGATGGAAGGAGTGGCGAGCGAAGCGGCGTCACTCGCCGGCCACTTGAAGCTCGGCCGTCTGATCGTCCTGTATGACTCGAACGACATTTCGCTGGACGGCGAGCTCAACTTGTCGTTTTCGGAAAACGTCGCCCAACGTTTCCAAGCGTACGGCTGGCAATATTTGCGCGTTGAGGACGGCAACAATATTGAAGAAATCGCCAAAGCGCTCGAGGAGGCGCGGACGGACCTCAGCCGGCCGACGCTCATTGAAGTAAAAACGACGATTGGCTACGGCGCGCCAAATAAAGCGGGCACGTCCGGCGTCCACGGCGCTCCGCTCGGCGCCCAAGAGGCGAAGTTGACGAAAGAAGCGTACCGCTGGACGTTTTCCGAAGATTTCTACGTGCCGGATGAAGTGTACGCTCATTTCCGGGAAACGGTGCAAGAAGCCGGAGCGAGAAAAGAAGCGGAGTGGAATGAGCGCTTCGTTGCTTACGAGCGGGCGCATCCGGAATTGGCCGCCGAGCTGAAGCAGGCGATTGAAGGGAAGCTTCCGGATGGCTGGGAAACATCGCTGCCGGTGTATGAAGCGGGCAAAAGCTTGGCGACCCGCTCATCGTCCGGGGAAGTGATCAACGCCATCGCCAAAGCGGTGCCGCATTGTTTGGCGGTTCGGCGGACTTGGCAAGCTCGAATAAAACGCTTATCAAAGGCGGCGGCAACTTCTTGCCGGACAGCTACGAAGGGCGCAACATTTGGTTTGGCGTGCGCGAGTTTGCCATGGGCGCGGCGTTAAACGGCATGGCGCTTCACGGCGGGTTGAAAGTGTTCGGCGGCACGTTCTTCGTGTTCTCCGACTACTTGCGCCCGGCGATTCGGCTGGCGGCGCTCATGGGCTTGCCGGTGACGTACGTGCTGACGCACGACAGCATCGCCGTCGGGGAAGACGGCCCGACGCATGAGCCGGTCGAGCATCTCGCTTCACTTCGGGCGATGCCGAACTTGTCAGTCATCCGGCCGGCTGACGCAAACGAAACGGCGGCCGCCTGGCGGCTGGCGCTCGAGTCGACGAACAAGCCGACTGCGCTCGTCTTGACGCGTCAAGATGTGCCGACATTGCCGACAACCGCTCAGTTGGCGTATGAAGGCGTGAAAAAAGGCGCGTACGTCGTTTCACCGGCGAAAAACGGCGCTCCGGAGGCGCTGTTGTTGGCGACTGGCTCGGAAGTCGGTCTGGCCGTCAAAGCGCAAGAAGCGCTCGCCGCTGAGGGCATCCATGTCTCCGTCATCAGCATGCCATCGTGGGACCGCTTCGAAGCGCAGCCAAAATCGTACCGCGATGAAGTGCTGCCGCCGGCCGTGACGAAGCGGCTCGCCATTGAAATGGGCGCCTCGCTCGGTTGGGAGCGCTACGTCGGCGCCGAGGGCGACATTTTGGCCATCGACCGATTCGGTGCTTCCGCTCCGGGAGAGAAAATCATGGCCGAGTATGGCTTTACGGTTGACAACGTCGTCCGCCGCACAAAAGCGCTGCTCGGCAAGTAA

> pQR1743

MAHSIEELAITTIRTLSIDAIEKAKSGHPGMPMGAAPMAYTLWTKFMNHNPANPNWFNRDRFVLSAGHGSMLLYSLLHLSGYDVTMDDLKQFRQWGSKTPGHPEYGHTPGVEATTGPLGQGIAMAVGMAMAERHLAAAYNRDGFDIINHYTYAICGDGDLMEGVASEAASLAGHLKLGRLIVLYDSNDISLDGELNLSFSENVAQRFQAYGWQYLRVEDGNNIEEIAKALEEARTDLSRPTLIEVKTTIGYGAPNKAGTSGVHGAPLGAQEAKLTKEAYRWTFSEDFYVPDEVYAHFRETVQEAGARKEAEWNERFVAYERAHPELAAELKQAIEGKLPDGWETSLPVYEAGKSLATRSSSGEVINAIAKAVPQLFGGSADLASSNKTLIKGGGNFLPDSYEGRNIWFGVREFAMGAALNGMALHGGLKVFGGTFFVFSDYLRPAIRLAALMGLPVTYVLTHDSIAVGEDGPTHEPVEHLASLRAMPNLSVIRPADANETAAAWRLALESTNKPTALVLTRQDVPTLPTTAQLAYEGVKKGAYVVSPAKNGAPEALLLATGSEVGLAVKAQEALAAEGIHVSVISMPSWDRFEAQPKSYRDEVLPPAVTKRLAIEMGASLGWERYVGAEGDILAIDRFGASAPGEKIMAEYGFTVDNVVRRTKALLGK

>pQR1744

ATGAACACCGGCACCCCAAAGACCCTGGACTGGTCTGATCTCGATAGACGTACCGTAGACGTGGTTCGTGCCCTGGCGATGGACGCGGTCGAAGAAGCGGGATCCGGGCACCCTGGAACCGCGATGAGTCTGGCGCCTGTGGCCTACCTGCTCTTCCAGAAGGTGATGCGGCACGATCCGACAGATCCGAAGTGGATCGGCCGCGACCGCTTCGTCCTGTCCTGCGGGCACTCCAGCCTCACGCTCTACATCCAGCTCTACCTGGCTGGCTACGGGCTGAGCCTGAACGACATCAAGCGGCTGCGCCAGTGGGGCAGCCTCACCCCGGGCCACCCCGAATACGGGCACACCGCCGGGGTGGAAACCACCACCGGCCCCTTGGGGCAGGGCATCGGCAACGCGGTCGGCATGGCCATGGCCGCCCGCCGGGAGCGGGGCCTGTTCGACCCGGACACCCCGATCGGGGAAAGCCCGTTCGACCACTACATCTACGTCCTGTGCTCTGACGGCGACGTCCAGGAGGGCATCAGCCACGAAGTAAGTGCCCTCGCCGGCACGCAGAAGCTCGGCAACCTCATCGTCATCTGGGACGACAACCGCATCTCCATCGAAGACGACACCCAGATCGCATTCACCGAAGACGTCGTCGCCCGCTACGCCGCCTACGGCTGGCACGTCCAAGAGGTCGAGTGGGTCGGCGAGGACGGCTCCTACCACGAAGACGTGGCGGCGCTGTACGACGCGATCCGGGCCGCCCAGGCGGAGACGGAACGTCCCTCTTTCATCCGGCTGCGCACCATCATCGGCTGGCCGTCCCCGAACAAGCAGAACACGGGGGCGATCCACGGCGCCGCGCTGGGGGCTGAAGAGGTCGCCGCCACCAAGCGGGTGCTGGGCCTCAACCCTGAGGCGCAGTTCGACGTGCCCAACGAGCTGCTGGAGCACGCCCGGGGCGTGGTGGCGCGGGGCCGCGCCGCCCGCCAGGAATGGGAGGCCTTGTTCGCCAAGTGGCGGGCCAACGCGGGCGAGCGTGCCGAACTGTTCGACCGGCTGATGGCAGGCTCGCTCCCGGACGGTTGGGAGAAGGCGATCCCGACCTTCGAGCCCAGCGCTAAGGGCATGGCCACCCGGAAAGCGTCCGGTGAGGTGCTGAGCGCGATCGCCCCGGTGCTGCCGGAGCTGTGGGGCGGCTCGGCGGACTTGGCCGGATCCAACAACACCACGCCTAAGGGCGAGCCGTCGTTCATCCCCGAGGAGCGGTCCACGAAGGCGTTCTCCGGCCACCGCTACGGCCGGGTGCTGCACTTCGGGATCCGTGAACACGGCATGGGGGCGATCCTCAACGGGATCGCGCTGCACGGCCCCACCCGCCCCTACGGTGGCACCTTCCTCGTGTTCAGCGACTACATGCGGCCGTCGGTGCGGCTGGCTGCCCTGATGAAGCTGCCGGTCACGTACGTGTGGACCCACGACTCGATCGGTCTGGGCGAAGACGGACCCACCCACCAGCCGGTGGAGCACCTGTGGTCGCTGCGCGCCATCCCCGGCCTGGCGGTGGTGCGTCCCGCCGACGCCAACGAGACGGCAGTGGCCTGGCGCACCATCCTGGAACGCAATGACGGCCCGGTGGCGCTCGCGCTGACCCGGCAGTCGGTTCCGGTTCTGGACCGCTCCGAGCTCGCCTCTGCGGAGCTGGTCTCCCGCGGCGGGTACATCCTGGCCGAAGCCAGCAACGGCCGTCCGGAGGCGATCATCATCGCCACCGGAAGTGAGGTGCAGATCGCGTTGGAGGCGCGTTCCCGCCTGGAGGAGTCGGGTACTCCTACCCGTGTGGTGTCGATGCCGTGCCTGGAGTGGTTCAACGAGCAGGACGACGCCTACCGCCAGCAGGTGCTTCCACCGTCGGTCCGGGTCCGGGTCTCCGTGGAAGCCGGGGTCGCCTTGGGCTGGCGCGAGCTGGTGGGCGAGTATGGCGAGTCGGTGAGTCTGGAACACTTCGGCGCTTCGGCTCCGTACGCGACTCTCTACGAGCAGTTCGGGCTCACCGCCGACCGGGTAGTGGCAGCCGTACACTCCAGCGCTGCCAAGCTCGGCGGTGACCGTGGATCAACGACCGGCAACTGA

>pQR1744

MNTGTPKTLDWSDLDRRTVDVVRALAMDAVEEAGSGHPGTAMSLAPVAYLLFQKVMRHDPTDPKWIGRDRFVLSCGHSSLTLYIQLYLAGYGLSLNDIKRLRQWGSLTPGHPEYGHTAGVETTTGPLGQGIGNAVGMAMAARRERGLFDPDTPIGESPFDHYIYVLCSDGDVQEGISHEVSALAGTQKLGNLIVIWDDNRISIEDDTQIAFTEDVVARYAAYGWHVQEVEWVGEDGSYHEDVAALYDAIRAAQAETERPSFIRLRTIIGWPSPNKQNTGAIHGAALGAEEVAATKRVLGLNPEAQFDVPNELLEHARGVVARGRAARQEWEALFAKWRANAGERAELFDRLMAGSLPDGWEKAIPTFEPSAKGMATRKASGEVLSAIAPVLPELWGGSADLAGSNNTTPKGEPSFIPEERSTKAFSGHRYGRVLHFGIREHGMGAILNGIALHGPTRPYGGTFLVFSDYMRPSVRLAALMKLPVTYVWTHDSIGLGEDGPTHQPVEHLWSLRAIPGLAVVRPADANETAVAWRTILERNDGPVALALTRQSVPVLDRSELASAELVSRGGYILAEASNGRPEAIIIATGSEVQIALEARSRLEESGTPTRVVSMPCLEWFNEQDDAYRQQVLPPSVRVRVSVEAGVALGWRELVGEYGESVSLEHFGASAPYATLYEQFGLTADRVVAAVHSSAAKLGGDRGSTTGN

>pQR1745

ATGGAAAGGTTTCCCTATGAAAAACTTCCAGAAAGCGAACTCAAAGAGTTGAAAGAACTCGGAAGGCTCTGCCGTGGCGACATACTGAAAATGACCTACATAGCTAACTCAGGCCATCCTGGAGGATCCATGTCTTCGATCGATCTTTATCTTACCGTCTTCAAGTACGCAAAACTCAGACCCGTCGATGATCCTGCAAGAGACAGAATCGTGATCAGCCATGGACACACTTCTCCGGGTGTCTACGCAGCTATGGCTCGTTTGGGGTTTGTCGATCTCGATGAAGTCCTCGCAGGATTCAGACACCCCGCTTCCGTTTTTGAAGGACACGTGACCCGAGGTGTTGGGATCATCGACTGGACAACCGGAAACCTCGGTCAGGGTCTTTCAGCCGGACTCGGTTTTGCCCTCGCATCCAGGTTCACAGGAAAAGATTACCACGTCTTTGTTCTCATGAGTGACGCAGAACAGGCAAAAGGACAGGTGGCGGAGGCAAGAAGAGTGGCGAAAAAGTACGGTGTCACGAATCTCACAGTGATCATCGACTACAACGACGCCCAGATCAGTGGCCGTGCCAGAGACGTCATGCCCGTGAACATAAAGGAAAACTACTTAGCGGACGGCTGGAGGGTCATCGAGATCGATGGGCACGACTACGAACAGATCTATCTCGCACTGAAAGAAGCGGTAGAAGACGAAC

TGAATCCCGTTGCCATAATCGCCAAAACGGTCATGGGAAAAGGCGTATCTTTCATGGAAAACGAGGTGAAATACCACGGAAAGCCTTTGAACAGAGAAGAACTCGAAAAAGCCCTCGCGGAACTCGGAATTGAAAACGATGTTGATGTGTACATCGAAAAAAGAAAACAACTTCCAGTGGAAAAACACAAGAAAGTCTACAAAACTTACCCGATCAAGATCGACACGGGAGAGCCCATCACCTACACCTCACCCACTGACAACAGAAGCGCATTCGGAAAAGCTATTCTGGATCTGGTGAAGAAGAACGTAAACAATCCAGAAACCACACCCATCGTCGCTGTGGACTGCGACCTGAAGGGATCGGTCAAACTCGACCTGCTCGACAAAGAGTTCCCTGAGAGACTCCTGGAAGTGGGCGTTCAGGAACACAACGCTGCCGCTATGGCGGGGGCACTCTCCGCAGAGGGTGTGATCACGTTCTTCGCTGATTTTGGTGTTTTTGGAATTTCTGAAACCTACAACCAGCACAGGCTGAACGCCATCAATGGAACGAACCTCAAAGTCGTTGTCACACACTGCGGACTCAACGTGGGAGAGGACGGAAAAACTCATCACGGACTCGACTACGTTTCCGGGCCGATGAACTGGTACGGTTTCAAAGTGATCGTCCCTGGTGATCCCAACCAGACGGATAGAGTTGTCAGATACGCCGCGAAGGAATACGGGAACTTCGTAATCGCCATGGGAAGATCTAAGCTTCCCATCATCCTCGATGAAAACGGGAAACCTTTCTTCGGAGAGGGTTACACCTTCGAATATGGGAAGATCGATGTCGTTAGAAAAGGTGACGACGCGGTGATCATAACTTACGGTTCTACACTCTGTGAAGCCGTAAATGCCGCAGACGAACTCAAGAAAGAAGGAGTAAACGTAGCCGTTCTGAATGTCTCCTGTCCGGTGGATCTCGATATAGAGACCTTGAAGATGGTCGATGGAAAACCCGTTCTCGTTGTGGAGGATCACAACGTTTTCACAGGACTTGGAAGCTTCCTTGGAACCACCCTTCTTGAAAACGGCATCATCCCGAAGAAATACGTGAGAGTAGGTGTTCCAGAATTCGCCGTGTCCGGCAGTTACACGATGCTCTACAAACTCTACGGCCTGGATAAAGATGGAATAATTTCCAGACTCAGAGAGATGCTCTAA

> pQR1745

MERFPYEKLPESELKELKELGRLCRGDILKMTYIANSGHPGGSMSSIDLYLTVFKYAKLRPVDDPARDRIVISHGHTSPGVYAAMARLGFVDLDEVLAGFRHPASVFEGHVTRGVGIIDWTTGNLGQGLSAGLGFALASRFTGKDYHVFVLMSDAEQAKGQVAEARRVAKKYGVTNLTVIIDYNDAQISGRARDVMPVNIKENYLADGWRVIEIDGHDYEQIYLALKEAVEDELNPVAIIAKTVMGKGVSFMENEVKYHGKPLNREELEKALAELGIENDVDVYIEKRKQLPVEKHKKVYKTYPIKIDTGEPITYTSPTDNRSAFGKAILDLVKKNVNNPETTPIVAVDCDLKGSVKLDLDKEFPERLLEVGVQEHNAAAMAGALSAEGVITFFADFGVFGISETYNQHRLNAINGTNLKVVVTHCGLNVGEDGKTHHGLDYVSGPMNWYGFKVIVPGDPNQTDRVVRYAAKEYGNFVIAMGRSKLPIILDENGKPFFGEGYTFEYGKIDVVRKGDDAVIITYGSTLCEAVNAADELKKEGVNVAVLNVSCPVDLDIETLKMVDGKPVLVVEDHNVFTGLGSFLGTTLLENGIIPKKYVRVGVPEFAVSGSYTMLYKLYGLDKDGIISRLREML

TAm DNA/ amino acid sequences

DNA sequences were retrieved from the NCBI database [5] and amino acid sequences were obtained through UniProtKB [6].

>TAm

ATGAAATTGGCAAAACGGGTGGCGTCGCTGACGCCATCGGCGACTTTGGCCATTACGGAGAAAGCAAAAGAACTAAAAGCGGCCGGGCATGACGTGATTGGTCTCGGAGCTGGCGAACCGGATTTCAACACGCCACAGCACATTCTTGATGCCGCCATCAAGGCAATGAACGAAGGACATACGAAATATACACCATCGGGCGGTTTGCCGGCGTTAAAGGAGGAAATTATAAAAAAATTCGCCCGCGACCAAGGCTTGGATTATGAGCCGGCTGAAGTGATTGTATGCGTCGGAGCGAAGCACGCCCTTTACACGCTGTTCCAAGTATTGCTCGATGAAGGCGACGAAGTGATCATTCCGACGCCATACTGGGTGAGCTATCCGGAACAAGTGAAACTGGCGGGCGGTGTTCCGGTTTACGTCGAAGGGCTTGAACAAAATCATTTTAAAATTACGCCGGAGCAGCTGAAACAGGCAATCACGCCGCGGACGAAAGCGGTTATCATCAACTCGCCGAGCAACCCGACTGGCATGATTTATACAGCCGAAGAGTTGAAGGCGCTTGGTGAGGTGTGCCTAGCGCATGGTGTATTGATCGTGTCAGATGAAATTTACGAAAAATTGACTTACGGCGGGGCGAAGCATGTGTCCATCGCTGAGTTGTCGCCGGAGCTGAAGGCGCAGACAGTCATCATTAACGGCGTGTCAAAGTCGCATTCGATGACGGGCTGGCGCATTGGTTATGCGGCGGGGCCGAAAGATATTATTAAGGCAATGACAGATTTGGCGAGCCACAGCACGTCCAACCCGACGTCAATCGCCCAATACGCGGCCATCGCTGCTTACAGCGGGCCGCAGGAGCCGGTCGAACAAATGCGCCAAGCGTTTGAACAACGGCTCAATATCATTTACGACAAGCTCGTGCAAATTCCAGGATTCACGTGCGTTAAGCCACAAGGGGCGTTTTATTTGTTCCCGAACGCCCGCGAAGCGGCTGCAATGGCCGGCTGCCGCACGGTCGACGAGTTCGTCGCTGCCTTGTTGGAGGAAGCGAAAGTCGCGCTTGTGCCCGGCTCTGGGTTTGGAGCGCCGGATAACGTTCGCTTGTCATACGCGACATCGCTCGATGCACTGGAAACCGCCGTGGAACGCATCCACCGGTTTATGGAAGCGCGCGCTTAA

>TAm

MKLAKRVASLTPSATLAITEKAKELKAAGHDVIGLGAGEPDFNTPQHILDAAIKAMNEGHTKYTPSGGLPALKEEIIKKFARDQGLDYEPAEVIVCVGAKHALYTLFQVLLDEGDEVIIPTPYWVSYPEQVKLAGGVPVYVEGLEQNHFKITPEQLKQAITPRTKAVIINSPSNPTGMITAEELKALGEVCLAHGVLIVSDEIYEKLTYGGAKHVSIAELSPELKAQTVIINGVSKSHSMTGWRIGYAAGPKDIIKAMTDLASHSTSNPTSIAQYAAIAAYSGPQEPVEQMRQAFEQRLNIIYDKLVQIPGFTCVKPQGAFYLFPNAREAAAMAGCRTVDEFVAALLEEAKVALVPGSGFGAPDNVRLSYATSLDALETAVERIHRFMEARA

>TAm

ATGTTCGAGGACACGCCCGCACCCTTTCCACCGCACATTCTGCTGACGCCCGGTCCGACACCGATTCACCCCCGGGCCCAGCGGGCGCTGCTGCGCGAGATGCTGGGGCACATGGACCCTGAGGTGTTCGCCCTGAACCGCGAGATCCAGGCGGACTTGCGGGTGATGTACGGGACGGGGCCCCAGACCTTTACGGCGCTGCTGGCGGGCACCGGGAGCCTGGGCATGGAGGCGGGCTTCGCCAACTTGGTGGAGAGGGGAGACGACGTGCTGATCTGCGTCAATGGTGCCTTTGGTCAGCGCATGGCCGAGATGGCGGCGCGCTACGGTGCGAATGTACGGCGGGTGACCGCGCCGCTGGGCGAGCCGATCGACCCGGCCGACGTGGCTGCGCGGTTGAGCGGCGCGCGGCTGGTGGCGGTGGTGCATGGGGAGACGAGCACGGGTGTGCTCAATCCGCTTCCGGAGATTGCCGAGGCCGTGCGCGGGAGCGGGGCATTGCTGGCCGTGGACGCCGTGACGACCGCCGGGATGGAACCCTTCCATATGGCGGACTGGGGCGTGGACTACGCCTATACCGGCGCGCAGAAGTGCCTCTCGGCACCGCCCGGCCTGGCCCCGGTGGCGATCAGCGACCGTGCTCTCGCTCGCCACGCGGCCCGCCGCACGCCCACGCCGCTGTGGTACTGCGATTTTGAGGGCCTGCGCGACTACTGGGACCGGCACAGCTACCACCACACGGTCCCGGTGAATCTGCACTACGCCTTCCACGCCGCCCTGCGCGCCGCACTCGAAGAAGGCCTCCAAGCCCGGCAGGCCCGCGTGCGCGACCTTGGCCAGGCGGTGCTGGCGGCCCTGACGCCGCTGGGCTTCACGCCGTATGTGGCCGATCCCGCCGCGCGGCTGCCCACCGTCTTGGCCCTGCGTCTTCCTCCCGGCTTCGACGACGCGGGCGTTCGCCAGGCCCTACGGGAACGCGGGATCAGCGTGACCGGCGGCCTGGGACCGACGGCAGGGCTGATCTGGCGTCTGGGCCTGATGGGGGAAGCGGCTCGCCCCGCGCCCTACCGCGCGCTGATGCTCGCCCTGGAAGACCTGCTGGGCGAGCGGGGCTTGGTGGCGCGCTTCGAGGAGGCGCTGGGCGTCGCGGCCTGA

>TAm

MFEDTPAPFPPHILLTPGPTPIHPRAQRALLREMLGHMDPEVFALNREIQADLRVMYGTGPQTFTALLAGTGSLGMEAGFANLVERGDDVLICVNGAFGQRMAEMAARYGANVRRVTAPLGEPIDPADVAARLSGARLVAVVHGETSTGVLNPLPEIAEAVRGSGALLAVDAVTTAGMEPFHMADWGVDYAYTGAQKCLSAPPGLAPVAISDRALARHAARRTPTPLWYCDFEGLRDYWDRHSYHHTVPVNLHYAFHAALRAALEEGLQARQARVRDLGQAVLAALTPLGFTPYVADPAARLPTVLALRLPPGFDDAGVRQALRERGISVTGGLGPTAGLIWRLGLMGEAARPAPYRALMLALEDLLGERGLVARFEEALGVAA

>pQR1746

ATGACCTCTCCTTTCCGCCTCTCCGCCCGCGCCCAGAGCCTCAAGCCGTCTGCGACAGTGGCGGTCACGTCCCGCGCCCTGGAACTCCAGCGTCAGGGCCTGGACGTGATTTCCATGAGCGTGGGCGAGCCGGATTTCGACACGCCGCCACATGTCAAGGCCGCCGGCATCGCCGCCATCGAGGAAGGCAAGACCAAATACACCCCGGTCAGCGGCATTCCCGAACTGCGCGAGGCCATCAGCGCCAAGTTTCGGCGCGAAAACGGCCTGGACTACGCGCCGAACGCCGTGACGGTAACGAGCGGCGGTAAACAGGCGCTGTTCAACGCCTTTTTCGCGTTGCTGAACCCCGGCGACGAGGTGCTGATTCCCGCGCCCCACTGGGTCAGCTACCCCGAAATGGTCGCGCTGACCGGCGCGGTGCCGGTAACCGTACCCACTACGCCGCAGCAGGGCTTTCAACTCGACCCGGACGCCCTCGCCGCCGCCATCACGCCGCGCACCCGCATGGTGATTCTCAACAGCCCCGGCAACCCGACGGGCGCGGTGTTTCCGCCGGAAACCTTGCGGGCGGTGGCCGACCTCGCCACGCAGCACGGCTTGATGATCGTCACCGACGAAATCTACGAGCACCTCGTCTACGACGCCGAGCAGGTCAGCATCGGCACCTACGCGCCGGAGCACACCCTGACCATCAATGGCGCGAGCAAAGCGTATGCCATGACCGGCTGGCGCATCGGCTACGCGGGCGGGCCGCGCGAGGTGATTGCCGCCATGAACGCGCTGCAATCGCAAAGCACCAGCAACGCCAGCAGCGTCAGCCAGTACGCCGCCCTCGCCGCGCTGGAACAGCACGAGGAAACCATGCGCTTCATCGACAGGGCCCGCACCGCCTACCGCGAACGGCGCGACCGCATCGTGGCGGGCCTCAACGCGCTGGGGTTGCCCACGCCCACGCCGCAAGGGGCCTTTTACGTGATGGCCGACACCCGCGCCATTCACACCGACGAACTCGAAGCCGCCCGCATCATTCTGGATGAGGCGCAGGTCGCCGTCGTGCCCGGCACCGATTTCGCCGCGCCGGGACAGGTGCGCCTGAGCTACGCGACCAGCATGGACAACATCGAGGAAGTGCTGCGGCGGCTGGAAGGGGTCGTGCGGCGCTAA

>pQR1746

MTSPFRLSARAQSLKPSATVAVTSRALELQRQGLDVISMSVGEPDFDTPPHVKAAGIAAIEEGKTKYTPVSGIPELREAISAKFRRENGLDYAPNAVTVTSGGKQALFNAFFALLNPGDEVLIPAPHWVSYPEMVALTGAVPVTVPTTPQQGFQLDPDALAAAITPRTRMVILNSPGNPTGAVFPPETLRAVADLATQHGLMIVTDEIYEHLVYDAEQVSIGTYAPEHTLTINGASKAYAMTGWRIGYAGGPREVIAAMNALQSQSTSNASSVSQYAALAALEQHEETMRFIDRARTAYRERRDRIVAGLNALGLPTPTPQGAFYVMADTRAIHTDELEAARIILDEAQVAVVPGTDFAAPGQVRLSYATSMDNIEEVLRRLEGVVRR

>pQR1748

ATGACTGACCGACCTCGTATCTCCGCACGCATCGGCGGTATCTCCGAGTCAGCGACCCTGGCGGTGGACGCCAAGGCCAAGGCCCTGAAGGCCGCTGGGCATCCCGTGATCGGCTTCGGCGCCGGGGAGCCTGACTTCCCCACGCCCGACTACATCGTGGAGGCAGCGGTCGCCGCCTGCCGCGACTCGCGCTTCCACCGCTACACCCCGGCGGGAGGCCTCCCCGAACTCAAGGAAGCCATCGCGGCTAAGACGCTGCGCGACTCCGGCTACCGGGTGGAGCCGAACCAAGTCCTGGTCACCAACGGCGGCAAGCAAGCGATCTACGAGGCGTTCGCCACGCTGCTCGATCCGGGCGACGAGGTCATCGTGATCGCGCCCTACTGGACCACCTACCCTGAATCGATCCGGCTGGCCGGAGGAACCCCGGTCTACGTGGTCACCGACGAGTCCACTGGCTACCTGGCCACGGTCGAGCAGCTGGAGGCGGCCCGCACCGACCGCACCAAGGTGCTGCTGTTCGTCTCCCCCTCGAACCCGACCGGCGCGGTGTACTCGCCCGAGCAGGTCCGCGAGATCGGCCGGTGGGCCCTCGAACACAACCTGTGGGTGCTCACCGACGAGATCTACGAGCACCTCGTCTACGGGGACGCCCGGTTCTCCTCGATGCCGGTGGAAGTTCCGGAACTGGCCGACCGCACCGTGGTGGTCAACGGGGTGGCCAAGACCTACGCCATGACCGGGTGGCGGGTCGGCTGGCTCATCGGCCCCGTGGACGTGGTCAAGGCTGCGACCAACCTGCAGTCGCACGCCACCTCCAATGTGGCCAACGTCTCGCAGGCCGCGGCTCTGGCAGCGGTCTCCGGCGACCTGTCGGCCGTGGAGGAGATGAAGCAGGCCTTCGACCGGCGGCGGCAGACCATTGTGCGGATGCTCAACGAGATCCCCGGTGTGGTGTGCCCCGAGCCCCAGGGCGCGTTCTACGCCTACCCGTCGGTCAAGGAGATCCTCGGCAAGGAGATCCGCGGTCAGCGTCCGCAGACCTCCAGCGAGCTGGCGTCGCTGATCCTGGAGCACGCCAAGGTCGCGGTGGTCCCGGGCGAGGCGTTCGGCACTCCGGGCTACCTGCGGTTGTCCTACGCGTTGAGCGACGCCGATCTGGTCGAAGGGGTCAGCCGGATCGCCAAGCTGCTGAGCGAAGCCCACTGA

>pQR1748

MTDRPRISARIGGISESATLAVDAKAKALKAAGHPVIGFGAGEPDFPTPDYIVEAAVAACRDSRFHRYTPAGGLPELKEAIAAKTLRDSGYRVEPNQVLVTNGGKQAIYEAFATLLDPGDEVIVIAPYWTTYPESIRLAGGTPVYVVTDESTGYLATVEQLEAARTDRTKVLLFVSPSNPTGAVYSPEQVREIGRWALEHNLWVLTDEIYEHLVYGDARFSSMPVEVPELADRTVVVNGVAKTYAMTGWRVGWLIGPVDVVKAATNLQSHATSNVANVSQAAALAAVSGDLSAVEEMKQAFDRRRQTIVRMLNEIPGVVCPEPQGAFYAYPSVKEILGKEIRGQRPQTSSELASLILEHAKVAVVPGEAFGTPGYLRLSYALSDADLVEGVSRIAKLLSEAH

>pQR1749

ATGGTATCCAGGAGAATATCAGAGATTCCCATATCGAAAACCATGGAACTCGACGCGAAGGCCAAAGCCCTCATAAAAAAGGGAGAAGACGTGATCAATCTAACGGCTGGTGAGCCGGATTTTCCCACACCGGAACCCGTCGTGGAAGAAGCGGTGAGATTTCTCCAGAAAGGAGAAGTGAAATACACAGATCCTCGTGGTATCTACGAACTCAGAGAGGGTATAGCGAAAAGGATAGGCGAGAGATACAAAAAAGATATCTCACCGGATCAGGTCGTGGTGACGAATGGAGCGAAACAGGCTCTGTTCAATGCTTTCATGGCCCTTCTCGATCCCGGTGACGAAGTGATCGTGTTTTCTCCCGTCTGGGTCAGCTACATTCCTCAGATCATCCTTGCTGGTGGCACGGTGAACGTGGTTGAGACGTTCATGAGTAAAAATTTCCAGCCCAGTCTGGAAGAGGTGGAAGGGCTTCTTGTTGGGAAAACGAAAGCCGTTCTTATCAACTCGCCGAACAATCCCACTGGTGTGGTGTACAGAAGAGAGTTCCTTGAAGGACTTGTGAGACTTGCCAAGAAGAGGAATTTCTACATAATCAGCGACGAAGTCTACGATTCCCTTGTTTACACGGATGAATTCACATCGATACTCGATGTTTCTGAAGGATTCGACCGGATAGTTTACATAAACGGCTTCTCGAAGTCTCACTCCATGACCGGCTGGAGGGTGGGTTACCTGATATCGAGCGAAAAAGTAGCGACCGCTGTGTCGAAGATCCAGTCTCACACCACCTCCTGTATCAACACGGTAGCACAGTACGCCGCCTTGAAGGCTCTGGAAGTGGACAACTCTTACATGGTTCAGACCTTTAAAGAAAGAAAAAATTTCGTGGTGGAAAGATTGAAAAAGATGGGTGTTAAGTTCGTGGAACCAGAAGGTGCGTTCTACCTCTTTTTCAAAGTCCGGGGTGACGATGTGAAATTCTGTGAAAGGCTCCTCGAAGAAAAGAAGGTTGCACTCGTTCCAGGATCCGCTTTTCTGAAGCCTGGATTTGTGAGGCTTTCTTTTGCCACATCTATAGAAAGACTTACGGAGGCGCTGGATAGAATTGAAGACTTCCTCAATTCTCGTTGA

>pQR1749

MVSRRISEIPISKTMELDAKAKALIKKGEDVINLTAGEPDFPTPEPVVEEAVRFLQKGEVKYTDPRGIYELREGIAKRIGERYKKDISPDQVVVTNGAKQALFNAFMALLDPGDEVIVFSPVWVSYIPQIILAGGTVNVVETFMSKNFQPSLEEVEGLLVGKTKAVLINSPNNPTGVVYRREFLEGLVRLAKKRNFYIISDEVYDSLVYTDEFTSILDVSEGFDRIVYINGFSKSHSMTGWRVGYLISSEKVATAVSKIQSHTTSCINTVAQYAALKALEVDNSYMVQTFKERKNFVVERLKKMGVKFVEPEGAFYLFFKVRGDDVKFCERLLEEKKVALVPGSAFLKPGFVRLSFATSIERLTEALDRIEDFLNSR

>pQR1751

ATGGCACCTGACCTGCGCCACCTGCACACCTTCGGCGAACTGGATCCGCCGCAACGCCTGTTGATGGGCCCCGGCCCGGTCAATGCGCATCCACGCGTGCTGCGTGCGATGGCGGCCGACCTGCTTGGCCAGTTCGACCCGGAAATGACCACCTACATGAACGAGGTGATGGCGCTGTACCGCCCCTTGTTCGGCACCCAGAACCGCTGGACCTTTCTGGTCGATGGCACGGCGCGCGCCGGCATCGAAGCCGCGCTGGTGTCGCTGGTGCAGCCGGGCGACCGTGTGCTGGTGATCAACTTCGGCCGCTTCGGTTTGTTGCTGACCGAAATCCTTGGCCGGCTCGGCGCCGACGTCCACACCGTGGATGCGCCGTGGGGCGAGGTGGTGCCGCTGGCGGCGATTGCCGAGGCGATCGCAAGCGTGGCACCCAAGCTGGTGGCCACCGTGCACGGCGACACCTCCACCACCATGGCGCAGCCGCTCGATGGCCTAGGCGCGCTATGCCGGGCGGCCGGCGCGCTGAGTTACGTAGACGCCACAGCCACCATCGGCGGCATGGACATCGCCAGCGACCGCTGGGAGGTGGACGTGGTCACCGCGGGGCTGCAGAAATGCCTGGGCGGGCCGTCCGGCTCGGCGCCGATCACTGTCTCTGCCGCGGCAGCGGAGGCGATCTTTGCGCGGCGGCATGTCGAACGCGGCATCGTGCGCGAGGACATCGCCAACGGCAGCGGCCCACGCATCGCCTCGAATTATTTCGACCTGGCGATGATCATGGATTACTGGTCCGACAAGCGCCTCAATCACCACACCGAAGCCACCACCATGCTGTACGGCGCGCGCGAATGCGCACGCGTGGCCTTGCAGGAAGGCCTGGAGGCGCGCTACGCCCGGCATGCGGCTGCCGGCCGCGCGGTCAGCGCCGGCGTGCGCGCACTGGGGCTGGAGGTGTTCGGCGACGATGCGCACCGCATGAGCAATGTCACCGGCGTGGTGATCCCGCACGGCGTCGACAGTGAAGCAGTGCGGCGGCGCATGCGCGAGGATTTCGAAATCGAGATCGGCACCGCGTTCGGCCCGCTGCAAGGCAGGATCTGGCGCATCGGTGCGATGGGCTACAACGCGATGAAGCACAAGGTGCTGCTCACCCTGGCCGCACTGGAAGCGGTGCTGCGCGCCGAGGGCTACGCGTGCACCCAAGGCCTGGCGGTCGAAGCCGCACGCGCCGCCTGGCATGCGGAGCCGGCTGCATGA

>pQR1751

MAPDLRHLHTFGELDPPQRLLMGPGPVNAHPRVLRAMAADLLGQFDPEMTTYMNEVMALYRPLFGTQNRWTFLVDGTARAGIEAALVSLVQPGDRVLVINFGRFGLLLTEILGRLGADVHTVDAPWGEVVPLAAIAEAIASVAPKLVATVHGDTSTTMAQPLDGLGALCRAAGALSYVDATATIGGMDIASDRWEVDVVTAGLQKCLGGPSGSAPITVSAAAAEAIFARRHVERGIVREDIANGSGPRIASNYFDLAMIMDYWSDKRLNHHTEATTMLYGARECARVALQEGLEARYARHAAAGRAVSAGVRALGLEVFGDDAHRMSNVTGVVIPHGVDSEAVRRRMREDFEIEIGTAFGPLQGRIWRIGAMGYNAMKHKVLLTLAALEAVLRAEGYACTQGLAVEAARAAWHAEPAA

>pQR1752

ATGGGAAAGTTTCTTAAGAAACACTACATAATGGCACCTGGACCAACACCAGTCCCAAACGATATTTTAACAGAAGGAGCGAAGGAAACAATACACCACAGAACACCTCAGTTTGTTTCCATAATGGAAGAGACCCTCGAAAGTGCAAAGTACATCTTTCAGACAAAACACAACGTGTACGCCTTTGCTTCCACAGGAACTGGCGCTATGGAAGCGGCGGTGGCGAATCTTGTGAGCCCTGGAGACAAAGTGATCGTGGTTGTGGCTGGAAAGTTCGGTGAAAGATGGAGAGAGCTCTGTCAGGCTTACGGTGCTGATATCGTAGAAATCGCCCTCGAATGGGGAGACGCGGTCACACCTGAACAGATCGAAGAGGCTCTCAACAAAAACCCCGATGCGAAGGTCGTCTTCACCACCTACAGTGAAACATCGACGGGTACAGTCATAGACCTCGAAGGAATTGCCAGAGTCACGAAGGAAAAAGACGTTGTTCTTGTGACAGACGCTGTCAGCGCTCTTGGAGCAGAACCACTGAAGATGGATGAATGGGGTGTGGATCTCGTTGTCACAGGTTCACAGAAGGGTTTGATGTTACCTCCAGGACTGGCGCTCATCTCTCTCAACGACAAAGCGTGGGGGCTCGTGGAAAAATCCAGATCTCCAAGGTACTACTTCGATCTGAGGGCCTACAGGAAATCTTACCCCGACAATCCTTACACCCCCGCAGTAAACATGATATACATGTTGAGAAAGGCTCTTCAGATGATAAAAGAGGAAGGCATAGAAAACGTATGGGAAAGGCACAGAATACTGGGAGACGCAACAAGAGCAGCGGTGAAAGCACTTGGACTGGAACTCCTCTCGAAAAGACCGGGAAACGTTGTAACAGCCGTGAAAGTGCCTGAGGGCATCGATGGAAAACAGATTCCCAAGATCATGAGAGACAAGTACGGTGTGACCATCGCCGGTGGACAGGCTAAACTCAAGGGAAAAATATTCAGGATAGCACACCTCGGATACATGTCACCTTTCGACACCATAACTGCCATTTCCGCTCTTGAATTAACCTTGAAGGAACTCGGTTATGAGTTCGAACTCGGAGTCGGTGTTAAGGCAGCCGAAGCTGTCTTCGCTAAAGAATTCATTGGGGAGTGA

>pQR1752

MGKFLKKHYIMAPGPTPVPNDILTEGAKETIHHRTPQFVSIMEETLESAKYIFQTKHNVYAFASTGTGAMEAAVANLVSPGDKVIVVVAGKFGERWRELCQAYGADIVEIALEWGDAVTPEQIEEALNKNPDAKVVFTTYSETSTGTVIDLEGIARVTKEKDVVLVTDAVSALGAEPLKMDEWGVDLVVTGSQKGLMLPPGLALISLNDKAWGLVEKSRSPRYYFDLRAYRKSYPDNPYTPAVNMIYMLRKALQMIKEEGIENVWERHRILGDATRAAVKALGLELLSKRPGNVVTAVKVPEGIDGKQIPKIMRDKYGVTIAGGQAKLKGKIFRIAHLGYMSPFDTITAISALELTLKELGYEFELGVGVKAAEAVFAKEFIGE

>pQR1755

ATGACTCAGATTTTTAATTTTAGCGCCGGTCCAGCAATGCTGCCGGTTGAAGTACTGCGTCGTGCTGAACAGGAATTGTGTAATTGGAATGGCCTGGGCACATCGGTTATGGAAATCAGCCACCGCAGTAAAGAGTTTATGCAGGTTGCCGCTGAATCCGAACAGGATCTGCGTGATTTGCTGAAAATCCCCTCCAACTACAAAGTGCTCTTTTGCCACGGCGGTGCTCGTGCGCAATTCGCCGCAGTGCCGTTAAATCTTCTGGGCGAACGCTCAACGGCCGACTACATCGACGGCGGGTATTGGGCGCACAGCGCAATCAATGAAGCAGAAAAATACTGCACGCCTAACGTGATTGACGTGAAAATGCGCGTGGGCGAACTGCGTGGCATTAAGCCGATGCGTGAATGGAAATTGTCTGATGACGCGGCGTTTGTGCATTACTGCCCGAATGAAACCATCGACGGTATTGCGATCGAAGAAGAGCCGGACTTTGGCGATAAAATTGTGGTCGCCGACTATTCTTCCAGCATCCTGTCTCGTCGTATTGATGTCAGCCGTTACGGCGTGATCTATGCCGGTGCGCAGAAAAATATCGGCCCTGCCGGCCTGACGCTGGTTATCGTACGTGAAGATTTGCTGGGCAAGGCGCGCCGTGAGCTGCCATCGATTCTGGATTACCAGGTTCTGGCGGACAATGACTCCATGTTTAACACGCCACCGACCTTTGCCTGGTACCTGTCCGGTATGGTCTTCAAATGGCTGAAAGAGTACGGCGGTCTGGCTGAAATGGAAAAACGTAACCAGGAGAAGGCTGACCTGCTGTATAGCGCGATTGACGGTAACGATTTCTATCGTAATGACGTTGCGGTAGCGAACCGTTCTCGCATGAATGTGCCATTCCTGTTGGCGGATTCTGCGCTGGATAAAGTCTTCCTGGAAGAATCAGTCGCTGCAGGTCTGCACGCGCTGAAAGGCCATCGCGTAGTAGGCGGCATGCGTGCCTCTATCTACAATGCGATGCCGTTGGAAGGCGTGAAAGTGCTGACGGAATTTATGGCTGACTTCGCTCGTCGCCACGGTTGA

>pQR1755

MTQIFNFSAGPAMLPVEVLRRAEQELCNWNGLGTSVMEISHRSKEFMQVAAESEQDLRDLLKIPSNYKVLFCHGGARAQFAAVPLNLLGERSTADYIDGGYWAHSAINEAEKYCTPNVIDVKMRVGELRGIKPMREWKLSDDAAFVHYCPNETIDGIAIEEEPDFGDKIVVADYSSSILSRRIDVSRYGVIYAGAQKNIGPAGLTLVIVREDLLGKARRELPSILDYQVLADNDSMFNTPPTFAWYLSGMVFKWLKEYGGLAEMEKRNQEKADLLYSAIDGNDFYRNDVAVANRSRMNVPFLLADSALDKVFLEESVAAGLHALKGHRVVGGMRASIYNAMPLEGVKVLTEFMADFARRHG

Subject area	Biology
More specific subject area	Biocatalysis
Type of data	Tables, text file, figures
How data was acquired	Experiments/ in-vitro assays and high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD)
Data format	Analyzed and tabulated
Experimental factors	All enzymes and substrates were freshly prepared before use
Experimental features	Experiments were carried out in triplicate
Data source location	United Kingdom, London, University College London (UCL)
Data accessibility	The data are accessible only within this article