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Literature DB >> 30227738

Chitosan-assisted permeabilization of ATP-biotin for live cell kinase-catalyzed biotinylation.

Ahmed E Fouda¹, D Maheeka Embogama¹, Vindya Ramanayake-Mudiyanselage¹, Mary Kay H Pflum¹.

Abstract

Kinases are essential cell signaling enzymes that phosphorylate protein substrates using ATP as the universal cosubstrate. A wide variety of ATP analogs have been used in kinase research, although the studies are limited by the cell impermeability of ATP. Here we describe the use of the cationic polymer deacetylated chitosan to permeabilize ATP analogs for live cell applications, including kinase-catalyzed biotinylation.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Substances：
ATP-biotin
Biotin
Chitosan

Year: 2018 PMID： 30227738 PMCID： PMC6335084 DOI： 10.2144/btn-2018-0076

Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993

33 in total

Review 1. Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry.

Authors: Jamie D Dunn; Gavin E Reid; Merlin L Bruening
Journal: Mass Spectrom Rev Date: 2010 Jan-Feb Impact factor: 10.946

2. Engineering unnatural nucleotide specificity for Rous sarcoma virus tyrosine kinase to uniquely label its direct substrates.

Authors: K Shah; Y Liu; C Deirmengian; K M Shokat
Journal: Proc Natl Acad Sci U S A Date: 1997-04-15 Impact factor: 11.205

Chitosan-assisted permeabilization of ATP-biotin for live cell kinase-catalyzed biotinylation.

Review 1. Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry.

2. Engineering unnatural nucleotide specificity for Rous sarcoma virus tyrosine kinase to uniquely label its direct substrates.

3. Insights into nucleotide binding in protein kinase A using fluorescent adenosine derivatives.

Review 4. The use of permeabilized cells to assay protein phosphorylation and catecholamine release.

5. Drug-induced nanocarrier assembly as a strategy for the cellular delivery of nucleotides and nucleotide analogues.

6. Kinase-Catalyzed Biotinylation.

7. A molecular gate which controls unnatural ATP analogue recognition by the tyrosine kinase v-Src.

8. The generality of kinase-catalyzed biotinylation.

9. Synthesis and reactivity of novel gamma-phosphate modified ATP analogues.

10. Assay of protein kinases using radiolabeled ATP: a protocol.

1. Kinase-Catalyzed Biotinylation to Map Cell Signaling Pathways: Application to Epidermal Growth Factor Signaling.

2. An Affinity-Based, Cysteine-Specific ATP Analog for Kinase-Catalyzed Crosslinking.

3. l-Lactate Dehydrogenase Identified as a Protein Tyrosine Phosphatase 1B Substrate by Using K-BIPS.