Liang Chen1,1, Jing Hu2,1, Lina Pan1, Xiaochun Yin1, Qibao Wang1, Hui Chen1. 1. Department of Endodontics, Jinan Stomatological Hospital, Jinan, Shandong, China. 2. Department of Stomatology, PLA Army General Hospital, Beijing, China.
Abstract
BACKGROUND: Despite progress in the treatment of oral squamous cell carcinoma (OSCC) over past years, the prognosis for OSCC patients remains dismal. MicroRNA-99a (miR-99a) has been found to involve in the development of many cancer types, but its clinical role in OSCC is unclear. OBJECTIVE: The aim of this study was to explore the clinical implications of serum miR-99a in OSCC. METHODS: This study detected serum miR-99a levels in 121 OSCC cases and 55 healthy controls by using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. RESULTS: The data showed that serum miR-99a expression was significantly decreased in OSCC patients compared with normal controls. OSCC patients with low miR-99a expression experienced more frequent poor differentiation and advanced clinical stage. Furthermore, in screening OSCC cases from normal controls, miR-99a could yield a receiver-operating characteristic (ROC) area under the curve (AUC) of 0.911 with 83.6% specificity and 80.2% sensitivity. Notably, patients with high miR-99a expression had longer overall survival and recurrence free survival. Finally, serum miR-99a was identified to be an independent prognostic indicator for OSCC. CONCLUSIONS: These results suggested that miR-99a might be a valuable marker for the prediction of early detection and prognosis in OSCC.
BACKGROUND: Despite progress in the treatment of oral squamous cell carcinoma (OSCC) over past years, the prognosis for OSCC patients remains dismal. MicroRNA-99a (miR-99a) has been found to involve in the development of many cancer types, but its clinical role in OSCC is unclear. OBJECTIVE: The aim of this study was to explore the clinical implications of serum miR-99a in OSCC. METHODS: This study detected serum miR-99a levels in 121 OSCC cases and 55 healthy controls by using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. RESULTS: The data showed that serum miR-99a expression was significantly decreased in OSCC patients compared with normal controls. OSCC patients with low miR-99a expression experienced more frequent poor differentiation and advanced clinical stage. Furthermore, in screening OSCC cases from normal controls, miR-99a could yield a receiver-operating characteristic (ROC) area under the curve (AUC) of 0.911 with 83.6% specificity and 80.2% sensitivity. Notably, patients with high miR-99a expression had longer overall survival and recurrence free survival. Finally, serum miR-99a was identified to be an independent prognostic indicator for OSCC. CONCLUSIONS: These results suggested that miR-99a might be a valuable marker for the prediction of early detection and prognosis in OSCC.
Authors: Arsinoe C Thomaidou; Panagiota Batsaki; Maria Adamaki; Maria Goulielmaki; Constantin N Baxevanis; Vassilis Zoumpourlis; Sotirios P Fortis Journal: Int J Mol Sci Date: 2022-07-26 Impact factor: 6.208
Authors: Óscar Rapado-González; Rafael López-López; José Luis López-Cedrún; Gabriel Triana-Martínez; Laura Muinelo-Romay; María Mercedes Suárez-Cunqueiro Journal: Cells Date: 2019-12-17 Impact factor: 6.600