Xing-Bo Mo1,2,3, Shu-Feng Lei1,2,3, Yong-Hong Zhang1,3, Huan Zhang1,3. 1. Jiangsu Key Laboratory of Preventive & Translational Medicine for Geriatric Diseases, Soochow University, 199 Renai Road, Suzhou, Jiangsu 215123, PR China. 2. Center for Genetic Epidemiology & Genomics, School of Public Health, Soochow University, 199 Renai Road, Suzhou, Jiangsu 215123, PR China. 3. Department of Epidemiology, School of Public Health, Soochow University, 199 Renai Road, Suzhou, Jiangsu 215123, PR China.
Abstract
AIM: To investigate the effects of m6A-single nucleotide polymorphisms (SNPs) on coronary artery disease (CAD). METHODS: We examined the association of m6A-SNPs with CAD in about 185,000 cases and controls and further performed eQTL and differential expression analyses to support the identified m6A-SNPs. RESULTS: Among the 4390 m6A-SNPs detected, 304 seemed to be associated with CAD (p < 0.05). SNP rs12286 was significantly associated with CAD at genome-wide level (p = 4.5 × 10-9). rs12286 was predicted to influence m6A methylation and have the potential to alter regulatory motifs binding, which may in turn regulate the expression of ADAMTS7 (p = 1.26 × 10-8). CONCLUSION: The present study found plenty of CAD-associated m6A-SNPs and demonstrated the potential functionality of the identified SNPs.
AIM: To investigate the effects of m6A-single nucleotide polymorphisms (SNPs) on coronary artery disease (CAD). METHODS: We examined the association of m6A-SNPs with CAD in about 185,000 cases and controls and further performed eQTL and differential expression analyses to support the identified m6A-SNPs. RESULTS: Among the 4390 m6A-SNPs detected, 304 seemed to be associated with CAD (p < 0.05). SNP rs12286 was significantly associated with CAD at genome-wide level (p = 4.5 × 10-9). rs12286 was predicted to influence m6A methylation and have the potential to alter regulatory motifs binding, which may in turn regulate the expression of ADAMTS7 (p = 1.26 × 10-8). CONCLUSION: The present study found plenty of CAD-associated m6A-SNPs and demonstrated the potential functionality of the identified SNPs.
Entities:
Keywords:
ADAMTS7; FTO; coronary artery disease; eQTL; gene expression; genome-wide association study; m6A; multifunctional variants; single nucleotide polymorphism; transcription regulation
Authors: Ru Li; Huan Zhang; Fan Tang; Chengcheng Duan; Dan Liu; Naqiong Wu; Yonghong Zhang; Laiyuan Wang; Xingbo Mo Journal: Front Cardiovasc Med Date: 2022-09-20