Jie Si-Tu1, Yi Cai2, Ting Feng3, Delin Yang4, Shunhui Yuan4, Xiaofang Yang4, Shuchen He4, Zhuoheng Li4, Yanwen Wang4, Yasi Tang4, Chunwei Ye5, Zhipeng Li6. 1. Department of Urology, The Third Affiliated Hospital of Sun Yet-sen University, Guangzhou, Guangdong Province 510630, PR China. 2. Department of Urology, Xiangya Hospital, Central South University, No.87 Xiangya Road, Changsha City, Hunan Province 410008, PR China. 3. People's Hospital of Yuxi City (The Sixth Affiliated Hospital of Kunming Medical University), Yuxi, Yunnan Province 653100, PR China. 4. The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China. 5. The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China. Electronic address: yechunweisci@sina.com. 6. The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China. Electronic address: pkzhh4@163.com.
Abstract
OBJECTIVE: Prostate cancer (PCa) is one of the most prevalent cancers affecting men worldwide. However, the biological functions of circRNAs in PCa are still largely unknown. METHODS: Real-time PCR (RT-PCR) and immunohistochemistry were performed to characterize the circ-102004 expression in both human PCa tissues and cell lines. The apoptosis and cell cycle status of prostate immortalized cell lines that were overexpressed with circ-102004 by transfection was analyzed using flow cytometry. The scratch test and the Transwell assay were conducted to evaluate the ability of transfected cells to migrate and invade. RNA sequencing, pathway analysis, and Western blotting were performed to probe the associations of circ-102004 with the classical cancer signaling pathways after functionally evaluating circ-102004 in a xenograft tumor model. RESULTS: In the present study, circ-102004 expression was found to be significantly higher in PCa samples than in the matched normal tissues. In functional experiments, circ-102004 is shown to play an oncogenic role in PCa by stimulating cancer cell migration and invasion. Circ-102004 overexpression was also accompanied by significant alterations in many signaling pathways, such as ERK, JNK, and Hedgehog, which are known to cause different types of cancers. CONCLUSIONS: Circ-102004 is a potential oncogenic gene that regulates the development and progression of PCa. This study provides a scientific basis for targeting circ-102004 for either diagnosis or therapy.
OBJECTIVE:Prostate cancer (PCa) is one of the most prevalent cancers affecting men worldwide. However, the biological functions of circRNAs in PCa are still largely unknown. METHODS: Real-time PCR (RT-PCR) and immunohistochemistry were performed to characterize the circ-102004 expression in both human PCa tissues and cell lines. The apoptosis and cell cycle status of prostate immortalized cell lines that were overexpressed with circ-102004 by transfection was analyzed using flow cytometry. The scratch test and the Transwell assay were conducted to evaluate the ability of transfected cells to migrate and invade. RNA sequencing, pathway analysis, and Western blotting were performed to probe the associations of circ-102004 with the classical cancer signaling pathways after functionally evaluating circ-102004 in a xenograft tumor model. RESULTS: In the present study, circ-102004 expression was found to be significantly higher in PCa samples than in the matched normal tissues. In functional experiments, circ-102004 is shown to play an oncogenic role in PCa by stimulating cancer cell migration and invasion. Circ-102004 overexpression was also accompanied by significant alterations in many signaling pathways, such as ERK, JNK, and Hedgehog, which are known to cause different types of cancers. CONCLUSIONS: Circ-102004 is a potential oncogenic gene that regulates the development and progression of PCa. This study provides a scientific basis for targeting circ-102004 for either diagnosis or therapy.