Literature DB >> 30218364

Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation.

Kris G Alavattam1,2,3, Hironori Abe4,5,6, Akihiko Sakashita4,5,6, Satoshi H Namekawa7,8,9.   

Abstract

A distinct form of X chromosome inactivation takes place during male meiosis, when the male sex chromosomes undergo a phenomenon known as meiotic sex chromosome inactivation (MSCI). MSCI is directed by DNA damage response signaling independent of Xist RNA to silence the transcriptional activity of the sex chromosomes, an essential event in male germ cell development. Here, we present protocols for the preparation and analyses of chromosome spread slides of mouse meiotic spermatocytes, thereby enabling a quick, inexpensive, and powerful cytological method to complement gene expression studies.

Entities:  

Keywords:  Cell staging; Cell suspension; Chromatin; Chromosomes; Cytology; DNA damage response; Immunofluorescence microscopy; Meiosis; Sex chromosomes; Slide protocol; Synapsis; Testis

Mesh：

Year:  2018        PMID： 30218364      PMCID： PMC8243718          DOI： 10.1007/978-1-4939-8766-5_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  25 in total

1.  Chromosome-wide nucleosome replacement and H3.3 incorporation during mammalian meiotic sex chromosome inactivation.

Authors:  Godfried W van der Heijden; Alwin A H A Derijck; Eszter Pósfai; Maud Giele; Pawel Pelczar; Liliana Ramos; Derick G Wansink; Johan van der Vlag; Antoine H F M Peters; Peter de Boer
Journal:  Nat Genet       Date:  2007-01-21       Impact factor: 38.330

2.  Flow cytometry purification of mouse meiotic cells.

Authors:  Irina V Getun; Bivian Torres; Philippe R J Bois
Journal:  J Vis Exp       Date:  2011-04-15       Impact factor: 1.355

3.  A drying-down technique for the spreading of mammalian meiocytes from the male and female germline.

Authors:  A H Peters; A W Plug; M J van Vugt; P de Boer
Journal:  Chromosome Res       Date:  1997-02       Impact factor: 5.239

Review 4.  Sex chromosome inactivation in germ cells: emerging roles of DNA damage response pathways.

Authors:  Yosuke Ichijima; Ho-Su Sin; Satoshi H Namekawa
Journal:  Cell Mol Life Sci       Date:  2012-03-02       Impact factor: 9.261

Review 5.  Sex chromosomes, recombination, and chromatin conformation.

Authors:  B D McKee; M A Handel
Journal:  Chromosoma       Date:  1993-01       Impact factor: 4.316

6.  FANCB is essential in the male germline and regulates H3K9 methylation on the sex chromosomes during meiosis.

Authors:  Yasuko Kato; Kris G Alavattam; Ho-Su Sin; Amom Ruhikanta Meetei; Qishen Pang; Paul R Andreassen; Satoshi H Namekawa
Journal:  Hum Mol Genet       Date:  2015-06-29       Impact factor: 6.150

7.  MDC1 directs chromosome-wide silencing of the sex chromosomes in male germ cells.

Authors:  Yosuke Ichijima; Misako Ichijima; Zhenkun Lou; André Nussenzweig; R Daniel Camerini-Otero; Junjie Chen; Paul R Andreassen; Satoshi H Namekawa
Journal:  Genes Dev       Date:  2011-05-01       Impact factor: 11.361

8.  SCML2 establishes the male germline epigenome through regulation of histone H2A ubiquitination.

Authors:  Kazuteru Hasegawa; Ho-Su Sin; So Maezawa; Tyler J Broering; Andrey V Kartashov; Kris G Alavattam; Yosuke Ichijima; Fan Zhang; W Clark Bacon; Kenneth D Greis; Paul R Andreassen; Artem Barski; Satoshi H Namekawa
Journal:  Dev Cell       Date:  2015-02-19       Impact factor: 12.270

9.  Pachytene asynapsis drives meiotic sex chromosome inactivation and leads to substantial postmeiotic repression in spermatids.

Authors:  James M A Turner; Shantha K Mahadevaiah; Peter J I Ellis; Michael J Mitchell; Paul S Burgoyne
Journal:  Dev Cell       Date:  2006-04       Impact factor: 12.270

10.  BAZ1B is dispensable for H2AX phosphorylation on Tyrosine 142 during spermatogenesis.

Authors:  Tyler J Broering; Yuan-Liang Wang; Ram Naresh Pandey; Rashmi S Hegde; Shao-Chun Wang; Satoshi H Namekawa
Journal:  Biol Open       Date:  2015-05-15       Impact factor: 2.422
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  6 in total

1.  Prenatal exposure to di-(2-ethylhexyl) phthalate and high-fat diet synergistically disrupts mouse fetal oogenesis and affects folliculogenesis†.

Authors:  Supipi Mirihagalle; Tianming You; Lois Suh; Chintan Patel; Liying Gao; Saniya Rattan; Huanyu Qiao
Journal:  Biol Reprod       Date:  2019-06-01       Impact factor: 4.285

2.  BRUCE preserves genomic stability in the male germline of mice.

Authors:  Lixiao Che; Kris G Alavattam; Peter J Stambrook; Satoshi H Namekawa; Chunying Du
Journal:  Cell Death Differ       Date:  2020-03-05       Impact factor: 15.828

3.  Licensing meiotic progression†.

Authors:  Kris G Alavattam; Satoshi H Namekawa
Journal:  Biol Reprod       Date:  2020-06-23       Impact factor: 4.285

4.  The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.

Authors:  Hironori Abe; Kris G Alavattam; Yueh-Chiang Hu; Qishen Pang; Paul R Andreassen; Rashmi S Hegde; Satoshi H Namekawa
Journal:  Curr Biol       Date:  2020-01-02       Impact factor: 10.834

5.  PRC1-mediated epigenetic programming is required to generate the ovarian reserve.

Authors:  Mengwen Hu; Yu-Han Yeh; Yasuhisa Munakata; Hironori Abe; Akihiko Sakashita; So Maezawa; Miguel Vidal; Haruhiko Koseki; Neil Hunter; Richard M Schultz; Satoshi H Namekawa
Journal:  Nat Commun       Date:  2022-08-10       Impact factor: 17.694

6.  Super-enhancer switching drives a burst in gene expression at the mitosis-to-meiosis transition.

Authors:  So Maezawa; Akihiko Sakashita; Masashi Yukawa; Xiaoting Chen; Kazuki Takahashi; Kris G Alavattam; Ippo Nakata; Matthew T Weirauch; Artem Barski; Satoshi H Namekawa
Journal:  Nat Struct Mol Biol       Date:  2020-09-07       Impact factor: 15.369
  6 in total

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