Literature DB >> 3021591

Molecular cloning and expression in Escherichia coli of the lethal factor gene of Bacillus anthracis.

D L Robertson, S H Leppla.   

Abstract

We have cloned and expressed in Escherichia coli the lethal factor (LF) gene of Bacillus anthracis. At least two of the six LF recombinant plasmids produce full-length LF protein. Transcription of the LF gene in E. coli appears to be under the control of its own B. anthracis promoter. Recombinant LF protein produced in E. coli remains intracellular and is not secreted. However, this LF protein is biochemically active and displays the same lethal effects as LF secreted by B. anthracis in the mouse macrophage assay. The LF gene, like that of the protective antigen gene, is present on the large B. anthracis toxin plasmid pXO1.

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Year:  1986        PMID： 3021591     DOI： 10.1016/0378-1119(86)90044-2

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  28 in total

1.  Autogenous regulation of the Bacillus anthracis pag operon.

Authors:  A R Hoffmaster; T M Koehler
Journal:  J Bacteriol       Date:  1999-08       Impact factor: 3.490

2.  atxA controls Bacillus anthracis capsule synthesis via acpA and a newly discovered regulator, acpB.

Authors:  Melissa Drysdale; Agathe Bourgogne; Susan G Hilsenbeck; Theresa M Koehler
Journal:  J Bacteriol       Date:  2004-01       Impact factor: 3.490

3.  Targeting HIV proteins to the major histocompatibility complex class I processing pathway with a novel gp120-anthrax toxin fusion protein.

Authors:  T J Goletz; K R Klimpel; N Arora; S H Leppla; J M Keith; J A Berzofsky
Journal:  Proc Natl Acad Sci U S A       Date:  1997-10-28       Impact factor: 11.205

4.  Capsule synthesis by Bacillus anthracis is required for dissemination in murine inhalation anthrax.

Authors:  Melissa Drysdale; Sara Heninger; Julie Hutt; Yahua Chen; C Rick Lyons; Theresa M Koehler
Journal:  EMBO J       Date:  2004-12-16       Impact factor: 11.598

Review 5.  Assembly and Function of the Bacillus anthracis S-Layer.

Authors:  Dominique Missiakas; Olaf Schneewind
Journal:  Annu Rev Microbiol       Date:  2017-09-08       Impact factor: 15.500

6.  Multicomponent anthrax toxin display and delivery using bacteriophage T4.

Authors:  Sathish B Shivachandra; Qin Li; Kristina K Peachman; Gary R Matyas; Stephen H Leppla; Carl R Alving; Mangala Rao; Venigalla B Rao
Journal:  Vaccine       Date:  2006-10-17       Impact factor: 3.641

7.  BslA, the S-layer adhesin of B. anthracis, is a virulence factor for anthrax pathogenesis.

Authors:  Justin Kern; Olaf Schneewind
Journal:  Mol Microbiol       Date:  2009-11-10       Impact factor: 3.501

8.  Regulation of the Bacillus anthracis protective antigen gene: CO2 and a trans-acting element activate transcription from one of two promoters.

Authors:  T M Koehler; Z Dai; M Kaufman-Yarbray
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

9.  The roles of AtxA orthologs in virulence of anthrax-like Bacillus cereus G9241.

Authors:  Jennifer M Scarff; Malik J Raynor; Yuliya I Seldina; Christy L Ventura; Theresa M Koehler; Alison D O'Brien
Journal:  Mol Microbiol       Date:  2016-09-04       Impact factor: 3.501

10.  Vaccine protection against Bacillus cereus-mediated respiratory anthrax-like disease in mice.

Authors:  So-Young Oh; Hannah Maier; Jay Schroeder; G Stefan Richter; Derek Elli; James M Musser; Lauriane E Quenee; Dominique M Missiakas; Olaf Schneewind
Journal:  Infect Immun       Date:  2013-01-14       Impact factor: 3.441
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