Nadin N Toaima1, Rasha H El-Owaidy2, Dina L Zaki3, Lerine B Eldin4. 1. Pediatric Endocrine Unit, Children's Hospital, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: nadin.toaima@med.asu.edu.eg. 2. Pediatric Allergy and Immunology Unit, Children's Hospital, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: rashahasan@med.asu.edu.eg. 3. Egyptian Ministry of Health Hospital, Cairo, Egypt. Electronic address: dinazaki25@yahoo.com. 4. Pediatric Endocrine Unit, Children's Hospital, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: lerinebahy@yahoo.com.
Abstract
INTRODUCTION: There is a possible association between obesity and infections. We sought to investigate phagocytic functions in obese children and their relation to serum leptin levels. METHODS: A cross sectional, controlled study was conducted, comprising 40 cases with simple visceral-type obesity. Subjects were evaluated for percentage of caloric intake, frequency and type of infections, body mass index (BMI) z score, in addition to complete blood counting, serum leptin assay (ELISA) and Dihydrorhodamine (DHR) flowcytometry. RESULTS: Cases were 21 males (52.5%) and 19 females (47.5%) with mean age 7.14 years±2.73 SD with median duration of obesity 4.2 years (IQR: 2-6). Cases had higher frequency of infections compared with controls (p<0.001). Serum leptin was significantly higher among cases (t=-12.391, p<0.001), while DHR results were comparable in the studied groups (p=0.067). Among cases, absolute lymphocytic count (ALC) correlated negatively with percentage of total caloric intake (p=0.045). Leptin levels correlated positively with frequency of infections (p=0.019) but negatively with ALC (p=0.043). DHR results showed weak negative correlations with serum leptin (p=0.177) and with BMI Z score (p=0.109). CONCLUSION: Obese children are posed at increased risk of infections and have higher serum leptin levels with possible negative effects of leptin on phagocytic functions.
INTRODUCTION: There is a possible association between obesity and infections. We sought to investigate phagocytic functions in obesechildren and their relation to serum leptin levels. METHODS: A cross sectional, controlled study was conducted, comprising 40 cases with simple visceral-type obesity. Subjects were evaluated for percentage of caloric intake, frequency and type of infections, body mass index (BMI) z score, in addition to complete blood counting, serum leptin assay (ELISA) and Dihydrorhodamine (DHR) flowcytometry. RESULTS: Cases were 21 males (52.5%) and 19 females (47.5%) with mean age 7.14 years±2.73 SD with median duration of obesity 4.2 years (IQR: 2-6). Cases had higher frequency of infections compared with controls (p<0.001). Serum leptin was significantly higher among cases (t=-12.391, p<0.001), while DHR results were comparable in the studied groups (p=0.067). Among cases, absolute lymphocytic count (ALC) correlated negatively with percentage of total caloric intake (p=0.045). Leptin levels correlated positively with frequency of infections (p=0.019) but negatively with ALC (p=0.043). DHR results showed weak negative correlations with serum leptin (p=0.177) and with BMI Z score (p=0.109). CONCLUSION:Obesechildren are posed at increased risk of infections and have higher serum leptin levels with possible negative effects of leptin on phagocytic functions.
Authors: Valter Paulo Neves Miranda; Paulo Roberto Dos Santos Amorim; Ronaldo Rocha Bastos; Karina Lúcia Ribeiro Canabrava; Márcio Vidigal Miranda Júnior; Fernanda Rocha Faria; Sylvia do Carmo Castro Franceschini; Maria do Carmo Gouveia Peluzio; Silvia Eloiza Priore Journal: Mediators Inflamm Date: 2020-07-09 Impact factor: 4.711