Yuhua Feng1, Meizuo Zhong1, Shan Zeng1, Leyuan Wang2, Ping Liu1, Xiangyu Xiao1, Yiping Liu1. 1. Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, PR China. 2. Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, Hunan, PR China.
Abstract
AIM: To analyze the expression profiles, clinicopathological features and chemotherapeutic efficacies of exosome-derived miRNAs in diffuse large B-cell lymphoma (DLBCL). MATERIALS & METHODS: Next-generation sequencing technique was performed to identify miRNA profiles in exosomes from parental and chemoresistant DLBCL cells. The results were validated by quantitative real-time PCR, and further analyzed by bioinformatics and statistical methods. RESULTS: We identified 37 significantly upregulated and 17 downregulated miRNAs. Of four upregulated miRNAs validated, we found miR-99a-5p and miR-125b-5p were significantly upregulated. Increased levels of exosomal miR-99a-5p and miR-125b-5p in DLBCL patients' serum were associated with shorter progression-free survival time, and they can predict chemotherapeutic efficacy. CONCLUSION: Exosomal miR-99a-5p and miR-125b-5p can serve as biomarkers for DLBCL.
AIM: To analyze the expression profiles, clinicopathological features and chemotherapeutic efficacies of exosome-derived miRNAs in diffuse large B-cell lymphoma (DLBCL). MATERIALS & METHODS: Next-generation sequencing technique was performed to identify miRNA profiles in exosomes from parental and chemoresistant DLBCL cells. The results were validated by quantitative real-time PCR, and further analyzed by bioinformatics and statistical methods. RESULTS: We identified 37 significantly upregulated and 17 downregulated miRNAs. Of four upregulated miRNAs validated, we found miR-99a-5p and miR-125b-5p were significantly upregulated. Increased levels of exosomal miR-99a-5p and miR-125b-5p in DLBCL patients' serum were associated with shorter progression-free survival time, and they can predict chemotherapeutic efficacy. CONCLUSION: Exosomal miR-99a-5p and miR-125b-5p can serve as biomarkers for DLBCL.
Entities:
Keywords:
chemotherapy resistance; diffuse large B-cell lymphoma; exosomes; miRNA
Authors: Angela Galardi; Marta Colletti; Virginia Di Paolo; Patrizia Vitullo; Loretta Antonetti; Ida Russo; Angela Di Giannatale Journal: Int J Mol Sci Date: 2019-09-17 Impact factor: 5.923