Gülnur Taşçi Bozbaş1, Mustafa Yilmaz2, Esra Paşaoğlu3, Gülcan Gürer1, Rasime Ivgin1, Buket Demirci4. 1. Department of Physical Medicine and Rehabilitation, Medical Faculty of Adnan Menderes University, Aydın, Turkey. 2. Department of Clinical Biochemistry, Medical Faculty of Adnan Menderes University, Aydın, Turkey. 3. Department of Pathology, Bagcilar Training and Research Hospital, Istanbul, Turkey. 4. Department of Medical Pharmacology, Medical Faculty of Adnan Menderes University, Aydın, Turkey.
Abstract
OBJECTIVES: This study aims to investigate the effectiveness and reliability of ozone (O3) in Freund's complete adjuvant (FCA)-induced arthritis, an animal model for rheumatoid arthritis. PATIENTS AND METHODS: Thirty-six four- to five-month-old male Wistar rats weighing between 274-420 gr were used in this study. Saline was injected into the hind paws of half of these rats, and FCA was injected into the other half. At the end of two weeks, 40 μg of O3 was administered to nine rats from each group twice a week for seven total doses. The rats were followed-up in terms of clinical findings. At the sixth week, the rats were sacrificed and serum malondialdehyde, glutathione peroxidase, and superoxide dismutase levels were measured. In addition, ankle joints were separated for histopathological examination. RESULTS: Significant improvement was observed in terms of hind-paw diameter, severity of arthritis, and histopathological findings of inflammation after O3 treatment in the group with FCA-induced arthritis. Although it was not quite significant, an upward trend was detected in oxidative stress markers with O3 treatment. CONCLUSION: This study, the first to investigate the effects of systemic O3 on the clinical and histopathological outcomes of rheumatoid arthritis, indicates that O3 is a highly effective and reliable treatment method in FCA-induced arthritis in animal models.
OBJECTIVES: This study aims to investigate the effectiveness and reliability of ozone (O3) in Freund's complete adjuvant (FCA)-induced arthritis, an animal model for rheumatoid arthritis. PATIENTS AND METHODS: Thirty-six four- to five-month-old male Wistar rats weighing between 274-420 gr were used in this study. Saline was injected into the hind paws of half of these rats, and FCA was injected into the other half. At the end of two weeks, 40 μg of O3 was administered to nine rats from each group twice a week for seven total doses. The rats were followed-up in terms of clinical findings. At the sixth week, the rats were sacrificed and serum malondialdehyde, glutathione peroxidase, and superoxide dismutase levels were measured. In addition, ankle joints were separated for histopathological examination. RESULTS: Significant improvement was observed in terms of hind-paw diameter, severity of arthritis, and histopathological findings of inflammation after O3 treatment in the group with FCA-induced arthritis. Although it was not quite significant, an upward trend was detected in oxidative stress markers with O3 treatment. CONCLUSION: This study, the first to investigate the effects of systemic O3 on the clinical and histopathological outcomes of rheumatoid arthritis, indicates that O3 is a highly effective and reliable treatment method in FCA-induced arthritis in animal models.
Authors: Hadi Abdollahzad; Mir Amir Aghdashi; Mohammad Asghari Jafarabadi; Beitollah Alipour Journal: Arch Med Res Date: 2015-09-03 Impact factor: 2.235
Authors: Arif Aslaner; Tuğrul Çakır; Betül Çelik; Uğur Doğan; Burhan Mayir; Cebrail Akyüz; Cemal Polat; Ahmet Baştürk; Vural Soyer; Süleyman Koç; Ahmet Özer Şehirli Journal: Int J Clin Exp Med Date: 2015-08-15