Approach to the Investigation of Nucleosome Structure by Using the Highly Emissive Nucleobase th dG-tC FRET Pair.

A distance- and orientation-factor-dependent FRET system is a useful and attractive approach to the investigation of the conformational dynamics of nucleosomes. In this study, the application of the highly emissive nucleobase th dG-tC FRET pair to 601 nucleosomes is reported. It was found that the th dG-tC FRET pair was successfully incorporated to 145 bp 601 sequences, and different FRET efficiencies were obtained for the designated donor and acceptor positions in the nucleosome.