| Literature DB >> 30204053 |
Abdullah D Alanazi1,2,3,4,5,6, Robert Puschendorf1,2,3,4,5,6, Bashir Salim1,2,3,4,5,6, Mohamed S Alyousif1,2,3,4,5,6, Ibrahim O Alanazi1,2,3,4,5,6, Hajri R Al-Shehri1,2,3,4,5,6.
Abstract
We conducted a cross-sectional study to detect trypanosome infections of horses and donkeys in the Riyadh Province of Saudi Arabia. DNA was extracted from blood samples collected from 368 horses and 142 donkeys, and subjected to universal first ribosomal internal transcribed spacer region (ITS1)-PCR followed by Trypanosoma evansi species-specific RoTat1.2-PCR. The universal ITS1-PCR revealed T. evansi infection in horses ( n = 12; 3.3%) and donkeys ( n = 4; 2.8%). There was no significant effect of sex or age on the prevalence of trypanosomiasis in horses or donkeys. Application of the RoTat1.2-PCR revealed that the RoTat1.2 VSG gene was absent from the positive ITS1-PCR samples of 3 horses and 1 donkey. This discrepancy could be explained by the circulation of T. evansi type B in Saudi Arabia; however, this suspicion requires confirmation.Entities:
Keywords: Equine; ITS1; PCR; RoTat1.2; Saudi Arabia; trypanosomiasis
Mesh：
Year: 2018 PMID： 30204053 PMCID： PMC6505846 DOI： 10.1177/1040638718798688
Source DB: PubMed Journal: J Vet Diagn Invest ISSN： 1040-6387 Impact factor: 1.279