Haiqin Wu1, Lulu Ye1, Xiaoxi Lu1, Shuang Xie1, Qian Yang1, Qinghua Yu1. 1. MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, Jiangsu, 210095, P. R. China.
Abstract
SCOPE: The intestinal mucosal barrier, including the mucus layer, protects against invasion of enteropathogens, thereby inhibiting infection. In this study, the protective effect of Lactobacillus on the intestinal barrier against Salmonella infection is investigated. The underlying mechanism of its effect, specifically on the regulation of goblet cells through the Notch pathway, is also elucidated. METHODS AND RESULTS: Here, the protective effect of Lactobacillus on alleviating changes in the intestinal barrier caused by Salmonella infection is explored. It has been found that Salmonella typhimurium colonizes the colon and damages colonic mucosa. However, Lactobacillus acidophilus ATCC 4356 alleviates the colitis caused by Salmonella infection. Moreover, S. typhimurium infection causes colonic crypt hyperplasia with increased PCNA+ cells, while L. acidophilus administration resolves these pathological changes. In addition, it has been further demonstrated that Salmonella results in severe colitis associated with goblet cells, and Lactobacillus improves colitis similarly associated with goblet cells. Salmonella infection induces goblet cell loss and reduces MUC2 expression by increasing Dll1, Dll4, and HES1 expression, while L. acidophilus reverses epithelial damage by balancing the Notch pathway. CONCLUSION: The study demonstrates that colitis improvement is controlled by Lactobacillus ATCC 4356 by regulation of the Notch pathway; this finding will be useful for prevention against animal S. typhimurium infection.
SCOPE: The intestinal mucosal barrier, including the mucus layer, protects against invasion of enteropathogens, thereby inhibiting infection. In this study, the protective effect of Lactobacillus on the intestinal barrier against Salmonella infection is investigated. The underlying mechanism of its effect, specifically on the regulation of goblet cells through the Notch pathway, is also elucidated. METHODS AND RESULTS: Here, the protective effect of Lactobacillus on alleviating changes in the intestinal barrier caused by Salmonella infection is explored. It has been found that Salmonella typhimurium colonizes the colon and damages colonic mucosa. However, Lactobacillus acidophilusATCC 4356 alleviates the colitis caused by Salmonella infection. Moreover, S. typhimuriuminfection causes colonic crypt hyperplasia with increased PCNA+ cells, while L. acidophilus administration resolves these pathological changes. In addition, it has been further demonstrated that Salmonella results in severe colitis associated with goblet cells, and Lactobacillus improves colitis similarly associated with goblet cells. Salmonella infection induces goblet cell loss and reduces MUC2 expression by increasing Dll1, Dll4, and HES1 expression, while L. acidophilus reverses epithelial damage by balancing the Notch pathway. CONCLUSION: The study demonstrates that colitis improvement is controlled by LactobacillusATCC 4356 by regulation of the Notch pathway; this finding will be useful for prevention against animal S. typhimuriuminfection.
Authors: Miroslava Anna Šefcová; Marco Larrea-Álvarez; César Marcelo Larrea-Álvarez; Viera Karaffová; David Ortega-Paredes; Christian Vinueza-Burgos; Zuzana Ševčíková; Mikuláš Levkut; Róbert Herich; Viera Revajová Journal: Animals (Basel) Date: 2021-01-19 Impact factor: 2.752
Authors: Hana Lee; Kwang Bo Jung; Ohman Kwon; Ye Seul Son; Eunho Choi; Won Dong Yu; Naeun Son; Jun Hyoung Jeon; Hana Jo; Haneol Yang; Yeong Rak Son; Chan-Seok Yun; Hyun-Soo Cho; Sang Kyu Kim; Dae-Soo Kim; Doo-Sang Park; Mi-Young Son Journal: Gut Microbes Date: 2022 Jan-Dec