Ali Akbar Nagavi-Alhoseiny1,2, Maryam Torshabi2, Morteza Rasoulianboroujeni3, Lobat Tayebi3, Fahimeh Sadat Tabatabaei1,2,3. 1. Dental Research Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2. Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3. Marquette University, School of Dentistry, Milwaukee, WI, 53233, USA.
Abstract
PURPOSE: In this work, the effects of sodium chloride (NaCl) on gene expression of planktonic Streptococcus mutans cells are investigated. Also assessed are the effects of NaCl on zeta potential of sound and demineralized dentin. METHODS: The relative level of glucosyltransferase B (gtfB), gtfC and gtfD transcription of S. mutans in the presence of NaCl was evaluated by quantitative polymerase chain reaction (qPCR). The osmolality of varying salt (NaCl) concentrations and their influence on the zeta potential of sound and demineralized dentin was investigated as well. RESULTS: NaCl significantly reduced the expression of gtfB and C genes in planktonic S. mutans; whereas, gtf D gene expression significantly increased in the presence of NaCl (P < 0.05). NaCl at concentrations of 37.5 mg/ml reduced zeta potential of demineralized dentin, while no significant decrease of zeta potential was found when sound dentin was exposed to this concentration. CONCLUSION: NaCl reduces the expression of some gtfs in S. mutans and increases negative potential charge of demineralized dentin.
PURPOSE: In this work, the effects of sodium chloride (NaCl) on gene expression of planktonic Streptococcus mutans cells are investigated. Also assessed are the effects of NaCl on zeta potential of sound and demineralized dentin. METHODS: The relative level of glucosyltransferase B (gtfB), gtfC and gtfD transcription of S. mutans in the presence of NaCl was evaluated by quantitative polymerase chain reaction (qPCR). The osmolality of varying salt (NaCl) concentrations and their influence on the zeta potential of sound and demineralized dentin was investigated as well. RESULTS: NaCl significantly reduced the expression of gtfB and C genes in planktonic S. mutans; whereas, gtf D gene expression significantly increased in the presence of NaCl (P < 0.05). NaCl at concentrations of 37.5 mg/ml reduced zeta potential of demineralized dentin, while no significant decrease of zeta potential was found when sound dentin was exposed to this concentration. CONCLUSION: NaCl reduces the expression of some gtfs in S. mutans and increases negative potential charge of demineralized dentin.
Authors: S V van der Waal; L W M van der Sluis; A R Özok; R A M Exterkate; J van Marle; P R Wesselink; J J de Soet Journal: Int Endod J Date: 2011-08-22 Impact factor: 5.264
Authors: W Krzyściak; A Jurczak; D Kościelniak; B Bystrowska; A Skalniak Journal: Eur J Clin Microbiol Infect Dis Date: 2013-10-24 Impact factor: 3.267