| Literature DB >> 30196502 |
Naoto Nemoto1,2, Shigefumi Kumachi3, Hidenao Arai3.
Abstract
Single-domain antibody (e.g., Nanobody, VHH antibody) is a promising scaffold for therapeutic and diagnostic reagents. To expand the range of target molecules, in vitro selection using cell-free display technologies such as cDNA display is useful and powerful because of their huge libraries and robust stability. We provide technical details for in vitro selection of single-domain antibodies using cDNA display.Keywords: Antibody engineering; Cell-free translation; Directed evolution; Evolutionary molecular engineering; In vitro selection; Nanobody; Protein engineering; Puromycin; VHH; cDNA display; mRNA display
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Year: 2018 PMID： 30196502 DOI： 10.1007/978-1-4939-8648-4_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745