Barbara M Crisol1, Camilla B Veiga2, Luciene Lenhare3, Renata R Braga1, Vagner R R Silva1, Adelino S R da Silva4, Dennys E Cintra2, Leandro P Moura5, José R Pauli5, Eduardo R Ropelle6. 1. Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas, Limeira, SP, Brazil. 2. Laboratory of Nutritional Genomics (LabGeN), School of Applied Sciences, University of Campinas, Limeira, SP, Brazil. 3. Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas, Limeira, SP, Brazil; Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil. 4. Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School, School of Physical Education and Sport of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil. 5. Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas, Limeira, SP, Brazil; CEPECE - Center of Research in Sport Sciences, School of Applied Sciences, University of Campinas (UNICAMP), Limeira, São Paulo, Brazil. 6. Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas, Limeira, SP, Brazil; Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil; CEPECE - Center of Research in Sport Sciences, School of Applied Sciences, University of Campinas (UNICAMP), Limeira, São Paulo, Brazil. Electronic address: eduardo.ropelle@fca.unicamp.br.
Abstract
AIMS: Nicotinamide Riboside (NR) is a NAD+ booster with wide physiological repercussion including the improvement on glucose and lipid homeostasis, increasing the life expectancy in mammals. However, the effects of NR on metabolism are only partially known. Here, we evaluated the effects of NR on the thermogenic response, highlighting the brown adipose tissue (BAT) in lean mice. MAIN METHODS: Male C57BL/67 mice were supplement with NR (400 mg/Kg/day) during 5 weeks. The Comprehensive Lab Animal Monitoring System (CLAMS) and thermographic images were used to evaluated the physiological effects of NR treatment. The BAT were extracted and analyzed by Western Blotting and qPCR. Also, bioinformatics analyses were performed to establish the connection between the NAD+ synthesis pathway in BAT and thermogenic response in several isogenic strains of BXD mice. KEY FINDINGS: Transcriptomic analysis revealed that genes involved in NAD+ synthesis (Nampt and Nmnat1) in the BAT were negatively correlated with body weight and fat mass. The heat map showed a strong positive correlation between Nampt and Ucp1 mRNA in BAT and body temperature in several strains of BXD lean mice. The experimental approaches demonstrated that oral NR supplementation reduced the abdominal visceral fat depots, with discrete impact on oxygen consumption in C57BL/6J mice. Interestingly, NR significantly increased the body temperature, and this phenomenon was accompanied by high levels of UCP1 protein content and Pgc1α mRNA in BAT. SIGNIFICANCE: This study demonstrated the oral NR supplementation was sufficient to induce the thermogenic response in lean mice changing the BAT metabolism.
AIMS: Nicotinamide Riboside (NR) is a NAD+ booster with wide physiological repercussion including the improvement on glucose and lipid homeostasis, increasing the life expectancy in mammals. However, the effects of NR on metabolism are only partially known. Here, we evaluated the effects of NR on the thermogenic response, highlighting the brown adipose tissue (BAT) in lean mice. MAIN METHODS: Male C57BL/67 mice were supplement with NR (400 mg/Kg/day) during 5 weeks. The Comprehensive Lab Animal Monitoring System (CLAMS) and thermographic images were used to evaluated the physiological effects of NR treatment. The BAT were extracted and analyzed by Western Blotting and qPCR. Also, bioinformatics analyses were performed to establish the connection between the NAD+ synthesis pathway in BAT and thermogenic response in several isogenic strains of BXD mice. KEY FINDINGS: Transcriptomic analysis revealed that genes involved in NAD+ synthesis (Nampt and Nmnat1) in the BAT were negatively correlated with body weight and fat mass. The heat map showed a strong positive correlation between Nampt and Ucp1 mRNA in BAT and body temperature in several strains of BXD lean mice. The experimental approaches demonstrated that oral NR supplementation reduced the abdominal visceral fat depots, with discrete impact on oxygen consumption in C57BL/6J mice. Interestingly, NR significantly increased the body temperature, and this phenomenon was accompanied by high levels of UCP1 protein content and Pgc1α mRNA in BAT. SIGNIFICANCE: This study demonstrated the oral NR supplementation was sufficient to induce the thermogenic response in lean mice changing the BAT metabolism.
Authors: Shintaro Yamaguchi; Michael P Franczyk; Maria Chondronikola; Nathan Qi; Subhadra C Gunawardana; Kelly L Stromsdorfer; Lane C Porter; David F Wozniak; Yo Sasaki; Nicholas Rensing; Michael Wong; David W Piston; Samuel Klein; Jun Yoshino Journal: Proc Natl Acad Sci U S A Date: 2019-11-06 Impact factor: 11.205
Authors: Francesco Di Virgilio; Simonetta Falzoni; Alba Clara Sarti; Paola Chiozzi; Valentina Vultaggio-Poma; Anna Lisa Giuliani Journal: Methods Mol Biol Date: 2022
Authors: Barbara M Crisol; Camilla B Veiga; Renata R Braga; Luciene Lenhare; Igor L Baptista; Rafael C Gaspar; Vitor R Muñoz; André V Cordeiro; Adelino S R da Silva; Dennys E Cintra; Leandro P Moura; José R Pauli; Eduardo R Ropelle Journal: Eur J Nutr Date: 2019-09-07 Impact factor: 5.614
Authors: Ashley S Williams; Timothy R Koves; Yasminye D Pettway; James A Draper; Dorothy H Slentz; Paul A Grimsrud; Olga R Ilkayeva; Deborah M Muoio Journal: iScience Date: 2021-12-16